##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AflIII      AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BsaAI       BsaBI       
BseAI       BsiWI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CpoI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
FbaI        FseI        FspI        HpaI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PflFI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SwaI        Tth111I     XcmI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII        2685  GACGTC               GACGT^C
AgeI           37  ACCGGT               A^CCGGT
AlwNI        2860  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
BbeI          126  GGCGCC               GGCGC^C
BbsI         2532  GTCTTC               GAAGAC(2/6)
BglI          180  GCCACCTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2502  AGATCT               A^GATCT
BpuAI        2532  GTCTTC               GAAGAC(2/6)
BsgI         2235  CTGCAC               GTGCAG(16/14)
BsiEI          39  CGGTCG               CG[GA][CT]^CG
BsmBI        2683  GAGACG               CGTCTC(1/5)
BspDI        1593  ATCGAT               AT^CGAT
BsrFI          37  ACCGGT               [GA]^CCGG[CT]
BstAPI       2095  GCAAGGAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          14  CCAGGGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I         37  ACCGGT               [GA]^CCGG[CT]
ClaI         1593  ATCGAT               AT^CGAT
DraII         263  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII       3567  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3735  CCATGG               C^C[GA][CT]GG
EcoNI        1172  CCTCTCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      263  GGGGCCT              [GA]G^G[ACGT]CC[CT]
HaeII         126  GGCGCC               [GA]GCGC^[CT]
KasI          126  GGCGCC               G^GCGCC
MspA1I       3201  CAGCTG               C[AC]G^C[GT]G
NarI          126  GGCGCC               GG^CGCC
NcoI         3735  CCATGG               C^CATGG
PaeR7I       2680  CTCGAG               C^TCGAG
PflMI         368  CCAGGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI          37  ACCGGT               A^CCGGT
PstI         3649  CTGCAG               CTGCA^G
PvuII        3201  CAGCTG               CAG^CTG
SapI          300  GCTCTTC              GCTCTTC(1/4)
ScaI          703  AGTACT               AGT^ACT
SfoI          126  GGCGCC               GGC^GCC
Van91I        368  CCAGGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2680  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

BanI          126  GGCGCC               G^G[CT][GA]CC
              586  GGTGCC              
BlpI          885  GCTAAGC              GC^T[ACGT]AGC
             2852  GCTCAGC             
Bpu1102I      885  GCTAAGC              GC^T[ACGT]AGC
             2852  GCTCAGC             
BsaI          987  GAGACC               GGTCTC(1/5)
             3636  GAGACC              
BsaWI          37  ACCGGT               [AT]^CCGG[AT]
             1737  ACCGGA              
BsrBI         111  CCGCTC               CCGCTC(-3/-3)
              250  GAGCGG              
BstYI        2502  AGATCT               [GA]^GATC[CT]
             2627  GGATCT              
CelII         885  GCTAAGC              GC^T[ACGT]AGC
             2852  GCTCAGC             
EarI          301  CTCTTC               CTCTTC(1/4)
             1236  GAAGAG              
EcoT22I      1789  ATGCAT               ATGCA^T
             3248  ATGCAT              
HgaI          107  GACGC                GACGC(5/10)
               50  GCGTC               
HincII        786  GTTGAC               GT[CT]^[GA]AC
             2588  GTCAAC              
HindII        786  GTTGAC               GT[CT]^[GA]AC
             2588  GTCAAC              
Ksp632I       301  CTCTTC               CTCTTC(1/4)
             1236  GAAGAG              
MflI         2502  AGATCT               [GA]^GATC[CT]
             2627  GGATCT              
NsiI         1789  ATGCAT               ATGCA^T
             3248  ATGCAT              
NspI           70  ACATGC               [GA]CATG^[CT]
             2592  ACATGC              
SacI          277  GAGCTC               GAGCT^C
             1364  GAGCTC              
SspI         3495  AATATT               AAT^ATT
             3870  AATATT              
StuI         1435  AGGCCT               AGG^CCT
             2194  AGGCCT              
TaqI         1594  TCGA                 T^CGA
             2681  TCGA                
TthHB8I      1594  TCGA                 T^CGA
             2681  TCGA                
XbaI         3451  TCTAGA               T^CTAGA
             3911  TCTAGA              
XhoII        2502  AGATCT               [GA]^GATC[CT]
             2627  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaHI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BsrGI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        EaeI        EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        Hin1I       HinP1I      
HindIII     HinfI       HpaII       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspI        MvaI        MvnI        MwoI        NciI        
NdeI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmaI        SmlI        SspBI       StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XmaCI       XmaI        XmnI