##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BbrPI BclI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BstBI BstEII
BstPI Bsu36I CelII ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI KpnI KspI MamI MfeI
MluI MroI MunI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacI SacII SfiI SfuI
SmaI SnaBI SpeI SphI SspBI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 745 AACGTT AA^CGTT
AflII 1716 CTTAAG C^TTAAG
Alw44I 3680 GTGCAC G^TGCAC
ApaLI 3680 GTGCAC G^TGCAC
AspHI 3680 GTGCAC G[AT]GC[AT]^C
AviII 618 TGCGCA TGC^GCA
BalI 503 TGGCCA TGG^CCA
BbeI 789 GGCGCC GGCGC^C
BbvCI 360 GCTGAGG CCTCAGC(-5/-2)
BcgI 765 CGAGCAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 859 GGATAC GTATCC(6/5)
BcnI 371 CCCGG CC^[GC]GG
BfrI 1716 CTTAAG C^TTAAG
BglI 19 GCCGGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 3613 TACGTG [CT]AC^GT[GA]
BsaWI 875 ACCGGA [AT]^CCGG[AT]
BsiHKAI 3680 GTGCAC G[AT]GC[AT]^C
BsmBI 1175 GAGACG CGTCTC(1/5)
BspHI 1166 TCATGA T^CATGA
BsrFI 19 GCCGGC [GA]^CCGG[CT]
Bst1107I 2871 GTATAC GTA^TAC
BstAPI 162 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2871 GTATAC GTA^TAC
Cfr10I 19 GCCGGC [GA]^CCGG[CT]
DsaI 2859 CCATGG C^C[GA][CT]GG
EaeI 503 TGGCCA [CT]^GGCC[GA]
EcoT22I 2736 ATGCAT ATGCA^T
FspI 618 TGCGCA TGC^GCA
HaeII 789 GGCGCC [GA]GCGC^[CT]
KasI 789 GGCGCC G^GCGCC
MluNI 503 TGGCCA TGG^CCA
MscI 503 TGGCCA TGG^CCA
NaeI 19 GCCGGC GCC^GGC
NarI 789 GGCGCC GG^CGCC
NciI 371 CCCGG CC^[GC]GG
NcoI 2859 CCATGG C^CATGG
NdeI 2114 CATATG CA^TATG
NgoMIV 19 GCCGGC G^CCGGC
NsiI 2736 ATGCAT ATGCA^T
PshAI 384 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 745 AACGTT AA^CGTT
RcaI 1166 TCATGA T^CATGA
SalI 1188 GTCGAC G^TCGAC
SapI 3241 GAAGAGC GCTCTTC(1/4)
SfoI 789 GGCGCC GGC^GCC
SgrAI 18 CGCCGGCG C[GA]^CCGG[CT]G
StuI 248 AGGCCT AGG^CCT
TaqI 1189 TCGA T^CGA
TthHB8I 1189 TCGA T^CGA
XcmI 983 CCATCTACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 940 GACGTC GACGT^C
1177 GACGTC
AlwNI 163 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
355 CAGAGGCTG
AvaI 403 CTCGGG C^[CT]CG[GA]G
763 CCCGAG
BanI 789 GGCGCC G^G[CT][GA]CC
3740 GGTGCC
BbsI 3757 GAAGAC GAAGAC(2/6)
3871 GTCTTC
BpuAI 3757 GAAGAC GAAGAC(2/6)
3871 GTCTTC
BsgI 1060 GTGCAG GTGCAG(16/14)
2782 GTGCAG
BsoBI 403 CTCGGG C^[CT]CG[GA]G
763 CCCGAG
BspLU11I 914 ACATGT A^CATGT
934 ACATGT
BsrDI 3835 CATTGC GCAATG(2/0)
3777 GCAATG
BstXI 800 CCAATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2955 CCATTATTTTGG
DraIII 280 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1204 CACCGAGTG
EcoNI 213 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
240 CCTGCAGGAGG
EcoRI 2455 GAATTC G^AATTC
2730 GAATTC
HgaI 921 GACGC GACGC(5/10)
2886 GCGTC
HindIII 1464 AAGCTT A^AGCTT
2500 AAGCTT
HpaI 2489 GTTAAC GTT^AAC
3224 GTTAAC
PpuMI 420 GGGACCC [GA]G^G[AT]CC[CT]
1691 AGGTCCC
SbfI 141 CCTGCAGG CCTGCA^GG
240 CCTGCAGG
Sse8387I 141 CCTGCAGG CCTGCA^GG
240 CCTGCAGG
SwaI 2145 ATTTAAAT ATTT^AAAT
2338 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApaI
ApoI Asp700I AspI AvaII BanII
BbvI BfaI BglII BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SexAI SfaNI SfcI
SmlI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
Van91I XhoII XmnI