##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccII Aor51HI ApaI
AscI Asp718I AspI AvaI AviII
AvrII BanI BanII BbeI BbvCI
BclI BglI BglII BlnI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstXI BstZ17I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FbaI FseI FspI
HaeII HindIII KasI KpnI KspI
MluI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PmeI PshAI PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SphI Sse8387I SspBI StuI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccIII 384 TCCGGA T^CCGGA
AclI 3690 AACGTT AA^CGTT
AcyI 1124 GGCGTC G[GA]^CG[CT]C
AgeI 1536 ACCGGT A^CCGGT
Alw44I 920 GTGCAC G^TGCAC
AlwNI 3558 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 920 GTGCAC G^TGCAC
BalI 1092 TGGCCA TGG^CCA
BamHI 158 GGATCC G^GATCC
BbrPI 3069 CACGTG CAC^GTG
BcgI 1437 GCAGCAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 904 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 904 GCTAAGC GC^T[ACGT]AGC
BsaBI 643 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1124 GGCGTC G[GA]^CG[CT]C
BseAI 384 TCCGGA T^CCGGA
BsgI 1609 CTGCAC GTGCAG(16/14)
BsmI 900 GAATGC GAATGC(1/-1)
BspEI 384 TCCGGA T^CCGGA
BspLU11I 3716 ACATGT A^CATGT
BstBI 3932 TTCGAA TT^CGAA
BstYI 158 GGATCC [GA]^GATC[CT]
CelII 904 GCTAAGC GC^T[ACGT]AGC
CfoI 2313 GCGC GCG^C
DraIII 4063 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1547 CCACGG C^C[GA][CT]GG
EcoNI 2074 CCTATGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 3624 CCAAGG C^C[AT][AT]GG
FauI 13 CCCGC CCCGC(4/6)
HgaI 1125 GCGTC GACGC(5/10)
HhaI 2313 GCGC GCG^C
Hin1I 1124 GGCGTC G[GA]^CG[CT]C
HinP1I 2313 GCGC G^CGC
HpaI 2817 GTTAAC GTT^AAC
MamI 643 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 158 GGATCC [GA]^GATC[CT]
MluNI 1092 TGGCCA TGG^CCA
MroI 384 TCCGGA T^CCGGA
MscI 1092 TGGCCA TGG^CCA
NdeI 2117 CATATG CA^TATG
NspV 3932 TTCGAA TT^CGAA
PinAI 1536 ACCGGT A^CCGGT
PmaCI 3069 CACGTG CAC^GTG
PmlI 3069 CACGTG CAC^GTG
Psp1406I 3690 AACGTT AA^CGTT
PstI 1636 CTGCAG CTGCA^G
ScaI 2897 AGTACT AGT^ACT
SfuI 3932 TTCGAA TT^CGAA
StyI 3624 CCAAGG C^C[AT][AT]GG
SwaI 3186 ATTTAAAT ATTT^AAAT
TaqI 3933 TCGA T^CGA
TthHB8I 3933 TCGA T^CGA
XcmI 1179 CCAGGCTTCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 158 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 2537 GTAGAC GT^[AC][GT]AC
3506 GTAGAC
AflII 2014 CTTAAG C^TTAAG
2023 CTTAAG
AhdI 1296 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2861 GACATCAAGTC
AocI 781 CCTAAGG CC^T[ACGT]AGG
852 CCTGAGG
AspEI 1296 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2861 GACATCAAGTC
BbsI 2869 GTCTTC GAAGAC(2/6)
3871 GTCTTC
BcnI 1404 CCCGG CC^[GC]GG
1460 CCCGG
BfrI 2014 CTTAAG C^TTAAG
2023 CTTAAG
BpmI 1432 CTCCAG CTGGAG(16/14)
1815 CTCCAG
BpuAI 2869 GTCTTC GAAGAC(2/6)
3871 GTCTTC
BsaI 2833 GAGACC GGTCTC(1/5)
224 GGTCTC
BsaWI 384 TCCGGA [AT]^CCGG[AT]
1536 ACCGGT
BsrFI 1415 ACCGGC [GA]^CCGG[CT]
1536 ACCGGT
Bsu36I 781 CCTAAGG CC^T[ACGT]AGG
852 CCTGAGG
Cfr10I 1415 ACCGGC [GA]^CCGG[CT]
1536 ACCGGT
EaeI 1092 TGGCCA [CT]^GGCC[GA]
1462 CGGCCA
Eam1105I 1296 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2861 GACATCAAGTC
Eco81I 781 CCTAAGG CC^T[ACGT]AGG
852 CCTGAGG
EcoRI 955 GAATTC G^AATTC
1583 GAATTC
EcoT22I 2131 ATGCAT ATGCA^T
3666 ATGCAT
MspA1I 1633 CAGCTG C[AC]G^C[GT]G
3569 CAGCTG
NciI 1404 CCCGG CC^[GC]GG
1460 CCCGG
NsiI 2131 ATGCAT ATGCA^T
3666 ATGCAT
PflMI 622 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4062 CCACACTGTGG
PpuMI 2305 GGGTCCT [GA]G^G[AT]CC[CT]
3283 AGGTCCT
PvuII 1633 CAGCTG CAG^CTG
3569 CAGCTG
SapI 1248 GAAGAGC GCTCTTC(1/4)
1408 GCTCTTC
SmlI 2014 CTTAAG C^T[CT][GA]AG
2023 CTTAAG
SnaBI 3247 TACGTA TAC^GTA
3454 TACGTA
Tsp45I 590 GTGAC ^GT[GC]AC
2803 GTCAC
Van91I 622 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4062 CCACACTGTGG
XbaI 1709 TCTAGA T^CTAGA
1832 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BbvI BciVI BfaI
BmyI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MnlI MseI MslI MspI
MunI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SpeI SspI TfiI Tru9I TseI
Tsp509I TspRI XmnI