##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BanI BanII BbeI BbsI
BcgI BclI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstUI BstYI CelII
CfoI Cfr10I ClaI CpoI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I KasI
KpnI KspI MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NlaIV NotI NruI
NsiI PacI PflFI PinAI PmeI
PpuMI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI Tth111I XbaI XcmI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AciI 3585 CCGC CCGC(-3/-1)
AlwI 3535 GATCC GGATC(4/5)
AocI 4030 CCTCAGG CC^T[ACGT]AGG
Asp700I 2516 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 1237 GGACC G^G[AT]CC
BalI 2991 TGGCCA TGG^CCA
BbrPI 667 CACGTG CAC^GTG
BbvCI 4146 GCTGAGG CCTCAGC(-5/-2)
BbvI 4543 GCTGC GCAGC(8/12)
BciVI 3820 GGATAC GTATCC(6/5)
BcnI 1318 CCGGG CC^[GC]GG
BsaBI 1658 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 332 GAGGAG GAGGAG(10/8)
BsmI 2298 GAATGC GAATGC(1/-1)
BspHI 4433 TCATGA T^CATGA
BspMI 2133 ACCTGC ACCTGC(4/8)
Bst1107I 3284 GTATAC GTA^TAC
BstAPI 4178 GCAAAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2431 GGTTACC G^GT[ACGT]ACC
BstPI 2431 GGTTACC G^GT[ACGT]ACC
BstZ17I 3284 GTATAC GTA^TAC
Bsu36I 4030 CCTCAGG CC^T[ACGT]AGG
Cac8I 4117 GCGTGC GC[ACGT]^[ACGT]GC
Cfr13I 1237 GGACC G^G[ACGT]CC
DraIII 1838 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3545 CCATGG C^C[GA][CT]GG
EaeI 2991 TGGCCA [CT]^GGCC[GA]
Eco81I 4030 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2431 GGTTACC G^GT[ACGT]ACC
EcoRV 392 GATATC GAT^ATC
Fnu4HI 4543 GCTGC GC^[ACGT]GC
HapII 1318 CCGG C^CGG
HindIII 1702 AAGCTT A^AGCTT
HpaI 2557 GTTAAC GTT^AAC
HpaII 1318 CCGG C^CGG
ItaI 4543 GCTGC GC^[ACGT]GC
MamI 1658 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2991 TGGCCA TGG^CCA
MscI 2991 TGGCCA TGG^CCA
MspA1I 2502 CAGCTG C[AC]G^C[GT]G
MspI 1318 CCGG C^CGG
NciI 1318 CCGGG CC^[GC]GG
NcoI 3545 CCATGG C^CATGG
NdeI 2079 CATATG CA^TATG
PaeR7I 4138 CTCGAG C^TCGAG
PmaCI 667 CACGTG CAC^GTG
PmlI 667 CACGTG CAC^GTG
PvuII 2502 CAGCTG CAG^CTG
RcaI 4433 TCATGA T^CATGA
Sau96I 1237 GGACC G^G[ACGT]CC
ScaI 4381 AGTACT AGT^ACT
SwaI 2996 ATTTAAAT ATTT^AAAT
TseI 4543 GCTGC G^C[AT]GC
XhoI 4138 CTCGAG C^TCGAG
XmnI 2516 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 3284 GTATAC GT^[AC][GT]AC
4476 GTAGAC
AflII 744 CTTAAG C^TTAAG
1339 CTTAAG
BfrI 744 CTTAAG C^TTAAG
1339 CTTAAG
BmyI 1275 GTGCCC G[AGT]GC[ACT]^C
2951 GGGCAC
BsaAI 667 CACGTG [CT]AC^GT[GA]
2365 TACGTG
BsgI 1027 CTGCAC GTGCAG(16/14)
2135 CTGCAC
BsmFI 1759 GGGAC GGGAC(10/14)
3430 GGGAC
Bsp1286I 1275 GTGCCC G[AGT]GC[ACT]^C
2951 GGGCAC
BstBI 971 TTCGAA TT^CGAA
3042 TTCGAA
HincII 2557 GTTAAC GT[CT]^[GA]AC
4059 GTCAAC
HindII 2557 GTTAAC GT[CT]^[GA]AC
4059 GTCAAC
NspV 971 TTCGAA TT^CGAA
3042 TTCGAA
PflMI 2173 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2436 CCATATCCTGG
SfuI 971 TTCGAA TT^CGAA
3042 TTCGAA
Van91I 2173 CCAAGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2436 CCATATCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
AseI AsnI AvaI BfaI BpmI
BsaJI BsiYI BslI BsmAI BsoBI
Bsp1407I BspLU11I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI DdeI
DpnII DraI EarI EcoRII EcoT14I
FokI HaeIII HinfI HphI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NspI PleI PshBI
RsaI Sau3AI ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I