##################################
# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BglI BlnI BpmI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsoBI BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI FseI FspI HapII HgaI
Hin1I HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PstI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AciI 306 GCGG CCGC(-3/-1)
AclI 3678 AACGTT AA^CGTT
AflIII 2217 ACATGT A^C[GA][CT]GT
BamHI 869 GGATCC G^GATCC
BanI 405 GGTGCC G^G[CT][GA]CC
BanII 12 GAGCTC G[GA]GC[CT]^C
BclI 1147 TGATCA T^GATCA
BlpI 1941 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1941 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 3790 TGTACA T^GTACA
BspHI 2713 TCATGA T^CATGA
BspLU11I 2217 ACATGT A^CATGT
BsrGI 3790 TGTACA T^GTACA
BsrI 634 CCAGT ACTGG(1/-1)
BstAPI 1620 GCAAACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1941 GCTCAGC GC^T[ACGT]AGC
EcoRV 2454 GATATC GAT^ATC
EcoT22I 1266 ATGCAT ATGCA^T
FauI 306 GCGGG CCCGC(4/6)
FbaI 1147 TGATCA T^GATCA
HaeII 947 AGCGCC [GA]GCGC^[CT]
HindIII 1460 AAGCTT A^AGCTT
MspA1I 896 CAGCTG C[AC]G^C[GT]G
NheI 2366 GCTAGC G^CTAGC
NsiI 1266 ATGCAT ATGCA^T
PflMI 1012 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1286 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3678 AACGTT AA^CGTT
PvuII 896 CAGCTG CAG^CTG
RcaI 2713 TCATGA T^CATGA
SacI 12 GAGCTC GAGCT^C
SapI 2025 GAAGAGC GCTCTTC(1/4)
SspBI 3790 TGTACA T^GTACA
SwaI 3306 ATTTAAAT ATTT^AAAT
Van91I 1012 CCAACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1174 TCTAGA T^CTAGA
XcmI 2630 CCACAGTTTAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 249 GTAGAC GT^[AC][GT]AC
2349 GTAGAC
AlwNI 1366 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
2659 CAGAAACTG
Asp700I 748 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2163 GAACTTCTTC
AspHI 12 GAGCTC G[AT]GC[AT]^C
819 GAGCAC
BglII 1889 AGATCT A^GATCT
3038 AGATCT
BmyI 12 GAGCTC G[AGT]GC[ACT]^C
819 GAGCAC
BseRI 86 CTCCTC GAGGAG(10/8)
1720 CTCCTC
BsiHKAI 12 GAGCTC G[AT]GC[AT]^C
819 GAGCAC
BsmI 156 GAATGC GAATGC(1/-1)
355 GCATTC
Bsp1286I 12 GAGCTC G[AGT]GC[ACT]^C
819 GAGCAC
BsrDI 2622 GCAATG GCAATG(2/0)
3250 GCAATG
BstF5I 1078 GGATG GGATG(2/0)
1835 GGATG
DraII 1480 AGGACCC [GA]G^G[ACGT]CC[CT]
3626 GGGTCCT
EcoNI 477 CCTTGTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1916 CCTTGCACAGG
EcoO109I 1480 AGGACCC [GA]G^G[ACGT]CC[CT]
3626 GGGTCCT
EcoT14I 1216 CCAAGG C^C[AT][AT]GG
2572 CCTTGG
FokI 1078 GGATG GGATG(9/13)
1835 GGATG
HaeIII 587 GGCC GG^CC
1220 GGCC
HpaI 374 GTTAAC GTT^AAC
1235 GTTAAC
MslI 913 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3305 CATTTAAATG
NspI 2217 ACATGT [GA]CATG^[CT]
2827 GCATGT
PpuMI 1480 AGGACCC [GA]G^G[AT]CC[CT]
3626 GGGTCCT
ScaI 1598 AGTACT AGT^ACT
2184 AGTACT
StyI 1216 CCAAGG C^C[AT][AT]GG
2572 CCTTGG
TaqI 566 TCGA T^CGA
680 TCGA
TthHB8I 566 TCGA T^CGA
680 TCGA
XmnI 748 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2163 GAACTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwI
ApoI AseI AsnI AvaII BbsI
BbvI BfaI BfrI BpuAI BsaJI
BsiYI BslI BsmAI BssKI BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeI NdeII
NlaIII NlaIV PleI PshBI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII