################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI AlwNI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BglI BlpI Bpu1102I BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SphI Sse8387I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 2613 GTATAC GT^[AC][GT]AC AflII 3070 CTTAAG C^TTAAG Alw44I 1966 GTGCAC G^TGCAC AlwI 3217 GGATC GGATC(4/5) ApaLI 1966 GTGCAC G^TGCAC AspHI 1966 GTGCAC G[AT]GC[AT]^C AvaII 1892 GGACC G^G[AT]CC AvrII 3009 CCTAGG C^CTAGG BanII 295 GAGCCC G[GA]GC[CT]^C BciVI 3024 GTATCC GTATCC(6/5) BclI 3126 TGATCA T^GATCA BfrI 3070 CTTAAG C^TTAAG BlnI 3009 CCTAGG C^CTAGG BsaAI 3018 TACGTA [CT]AC^GT[GA] BsaI 3917 GGTCTC GGTCTC(1/5) BsaJI 3009 CCTAGG C^C[ACGT][ACGT]GG BsiHKAI 1966 GTGCAC G[AT]GC[AT]^C BspMI 303 ACCTGC ACCTGC(4/8) Bst1107I 2613 GTATAC GTA^TAC BstAPI 9 GCAAACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 59 CCAGCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2613 GTATAC GTA^TAC DrdI 3357 GACCCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 2192 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 3009 CCTAGG C^C[AT][AT]GG FauI 1091 GCGGG CCCGC(4/6) FbaI 3126 TGATCA T^GATCA HincII 2537 GTCAAC GT[CT]^[GA]AC HindII 2537 GTCAAC GT[CT]^[GA]AC HindIII 1097 AAGCTT A^AGCTT MaeII 3019 ACGT A^CGT MspA1I 60 CAGCTG C[AC]G^C[GT]G PflMI 3532 CCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1891 AGGACCC [GA]G^G[AT]CC[CT] PvuII 60 CAGCTG CAG^CTG SapI 565 GAAGAGC GCTCTTC(1/4) ScaI 3443 AGTACT AGT^ACT SnaBI 3018 TACGTA TAC^GTA SpeI 505 ACTAGT A^CTAGT StyI 3009 CCTAGG C^C[AT][AT]GG TaqI 2458 TCGA T^CGA TthHB8I 2458 TCGA T^CGA Van91I 3532 CCATATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AciI 1443 CCGC CCGC(-3/-1) 1091 GCGG AflIII 2124 ACATGT A^C[GA][CT]GT 3430 ACATGT BbsI 495 GAAGAC GAAGAC(2/6) 1218 GAAGAC BglII 2995 AGATCT A^GATCT 3236 AGATCT BpuAI 495 GAAGAC GAAGAC(2/6) 1218 GAAGAC BspLU11I 2124 ACATGT A^CATGT 3430 ACATGT Cfr13I 1892 GGACC G^G[ACGT]CC 2330 GGCCC DraII 1891 AGGACCC [GA]G^G[ACGT]CC[CT] 2329 AGGCCCT EcoO109I 1891 AGGACCC [GA]G^G[ACGT]CC[CT] 2329 AGGCCCT NheI 3245 GCTAGC G^CTAGC 3582 GCTAGC NlaIV 294 GGAGCC GG[ACGT]^[ACGT]CC 1892 GGACCC Sau96I 1892 GGACC G^G[ACGT]CC 2330 GGCCC SfcI 1353 CTATAG C^T[GA][CT]AG 3119 CTGTAG SwaI 614 ATTTAAAT ATTT^AAAT 2082 ATTTAAAT Tsp45I 2128 GTCAC ^GT[GC]AC 3523 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI Asp700I BbvI BfaI BmyI BpmI BsaBI BsiYI BslI BsmAI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII DraI EarI EcoRI EcoRII EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MamI MboI MboII MflI MnlI MseI MslI MvaI MwoI NdeII NlaIII NsiI NspI PleI PshBI RsaI Sau3AI ScrFI SfaNI SmlI SspBI SspI StuI TfiI Tru9I TseI Tsp509I TspRI XhoII XmnI