##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        AlwNI       AocI        
Aor51HI     ApaI        AscI        Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BcnI        BglI        BlpI        Bpu1102I    BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsoBI       BspDI       
BspEI       BspHI       BsrBI       BsrFI       BssHII      
BssSI       BstBI       BstEII      BstPI       BstUI       
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraIII      DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaI        HpaII       KasI        KpnI        KspI        
MfeI        MluI        MluNI       MroI        MscI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NotI        
NruI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SphI        Sse8387I    Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI         2613  GTATAC               GT^[AC][GT]AC
AflII        3070  CTTAAG               C^TTAAG
Alw44I       1966  GTGCAC               G^TGCAC
AlwI         3217  GGATC                GGATC(4/5)
ApaLI        1966  GTGCAC               G^TGCAC
AspHI        1966  GTGCAC               G[AT]GC[AT]^C
AvaII        1892  GGACC                G^G[AT]CC
AvrII        3009  CCTAGG               C^CTAGG
BanII         295  GAGCCC               G[GA]GC[CT]^C
BciVI        3024  GTATCC               GTATCC(6/5)
BclI         3126  TGATCA               T^GATCA
BfrI         3070  CTTAAG               C^TTAAG
BlnI         3009  CCTAGG               C^CTAGG
BsaAI        3018  TACGTA               [CT]AC^GT[GA]
BsaI         3917  GGTCTC               GGTCTC(1/5)
BsaJI        3009  CCTAGG               C^C[ACGT][ACGT]GG
BsiHKAI      1966  GTGCAC               G[AT]GC[AT]^C
BspMI         303  ACCTGC               ACCTGC(4/8)
Bst1107I     2613  GTATAC               GTA^TAC
BstAPI          9  GCAAACTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI          59  CCAGCTGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      2613  GTATAC               GTA^TAC
DrdI         3357  GACCCAGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        2192  CCTTCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I      3009  CCTAGG               C^C[AT][AT]GG
FauI         1091  GCGGG                CCCGC(4/6)
FbaI         3126  TGATCA               T^GATCA
HincII       2537  GTCAAC               GT[CT]^[GA]AC
HindII       2537  GTCAAC               GT[CT]^[GA]AC
HindIII      1097  AAGCTT               A^AGCTT
MaeII        3019  ACGT                 A^CGT
MspA1I         60  CAGCTG               C[AC]G^C[GT]G
PflMI        3532  CCATATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1891  AGGACCC              [GA]G^G[AT]CC[CT]
PvuII          60  CAGCTG               CAG^CTG
SapI          565  GAAGAGC              GCTCTTC(1/4)
ScaI         3443  AGTACT               AGT^ACT
SnaBI        3018  TACGTA               TAC^GTA
SpeI          505  ACTAGT               A^CTAGT
StyI         3009  CCTAGG               C^C[AT][AT]GG
TaqI         2458  TCGA                 T^CGA
TthHB8I      2458  TCGA                 T^CGA
Van91I       3532  CCATATTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AciI         1443  CCGC                 CCGC(-3/-1)
             1091  GCGG                
AflIII       2124  ACATGT               A^C[GA][CT]GT
             3430  ACATGT              
BbsI          495  GAAGAC               GAAGAC(2/6)
             1218  GAAGAC              
BglII        2995  AGATCT               A^GATCT
             3236  AGATCT              
BpuAI         495  GAAGAC               GAAGAC(2/6)
             1218  GAAGAC              
BspLU11I     2124  ACATGT               A^CATGT
             3430  ACATGT              
Cfr13I       1892  GGACC                G^G[ACGT]CC
             2330  GGCCC               
DraII        1891  AGGACCC              [GA]G^G[ACGT]CC[CT]
             2329  AGGCCCT             
EcoO109I     1891  AGGACCC              [GA]G^G[ACGT]CC[CT]
             2329  AGGCCCT             
NheI         3245  GCTAGC               G^CTAGC
             3582  GCTAGC              
NlaIV         294  GGAGCC               GG[ACGT]^[ACGT]CC
             1892  GGACCC              
Sau96I       1892  GGACC                G^G[ACGT]CC
             2330  GGCCC               
SfcI         1353  CTATAG               C^T[GA][CT]AG
             3119  CTGTAG              
SwaI          614  ATTTAAAT             ATTT^AAAT
             2082  ATTTAAAT            
Tsp45I       2128  GTCAC                ^GT[GC]AC
             3523  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AcsI        AfaI        AluI        ApoI        AseI        
AsnI        Asp700I     BbvI        BfaI        BmyI        
BpmI        BsaBI       BsiYI       BslI        BsmAI       
BsmI        Bsp1286I    Bsp1407I    BsrDI       BsrGI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       DdeI        DpnII       DraI        EarI        
EcoRI       EcoRII      EcoT22I     Fnu4HI      FokI        
HaeIII      HinfI       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MamI        MboI        MboII       
MflI        MnlI        MseI        MslI        MvaI        
MwoI        NdeII       NlaIII      NsiI        NspI        
PleI        PshBI       RsaI        Sau3AI      ScrFI       
SfaNI       SmlI        SspBI       SspI        StuI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XhoII       XmnI