##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AvaI AviII AvrII
BanII BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
BspEI BsrBI BsrFI BssHII BssSI
BstBI BstUI Bsu36I CelII CfoI
Cfr10I CpoI DraII DraIII DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I Van91I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AciI 58 GCGG CCGC(-3/-1)
AflIII 1867 ACATGT A^C[GA][CT]GT
AlwNI 2136 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 3676 ATTAAT AT^TAAT
AsnI 3676 ATTAAT AT^TAAT
Asp700I 2465 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 858 GTGCTC G[AT]GC[AT]^C
AspI 1564 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1378 GGATCC G^GATCC
BciVI 2653 GGATAC GTATCC(6/5)
BglII 2231 AGATCT A^GATCT
BpmI 1137 CTCCAG CTGGAG(16/14)
BsaBI 2997 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 1098 CTGCAC GTGCAG(16/14)
BsiHKAI 858 GTGCTC G[AT]GC[AT]^C
Bsp1407I 1870 TGTACA T^GTACA
BspDI 1631 ATCGAT AT^CGAT
BspLU11I 1867 ACATGT A^CATGT
BsrGI 1870 TGTACA T^GTACA
Bst1107I 2975 GTATAC GTA^TAC
BstAPI 522 GCATTCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3138 CCAGTAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2975 GTATAC GTA^TAC
ClaI 1631 ATCGAT AT^CGAT
DrdI 1323 GACAGCATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1519 CCTTGTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1814 GAATTC G^AATTC
EcoRV 1655 GATATC GAT^ATC
EcoT14I 1920 CCAAGG C^C[AT][AT]GG
HapII 1252 CCGG C^CGG
HincII 3759 GTCAAC GT[CT]^[GA]AC
HindII 3759 GTCAAC GT[CT]^[GA]AC
HpaII 1252 CCGG C^CGG
MamI 2997 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 252 CAATTG C^AATTG
MspI 1252 CCGG C^CGG
MunI 252 CAATTG C^AATTG
NspI 1867 ACATGT [GA]CATG^[CT]
PflFI 1564 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3676 ATTAAT AT^TAAT
ScaI 3167 AGTACT AGT^ACT
SexAI 3210 ACCAGGT A^CC[AT]GGT
SpeI 4142 ACTAGT A^CTAGT
SspBI 1870 TGTACA T^GTACA
StyI 1920 CCAAGG C^C[AT][AT]GG
SwaI 2869 ATTTAAAT ATTT^AAAT
Tth111I 1564 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2090 CCATCTTCATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2465 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaII 797 GGACC G^G[AT]CC
1924 GGACC
BanI 2549 GGTGCC G^G[CT][GA]CC
3686 GGCACC
BmyI 858 GTGCTC G[AGT]GC[ACT]^C
3685 GGGCAC
BseRI 2145 CTCCTC GAGGAG(10/8)
2518 CTCCTC
BsmAI 2606 GAGAC GTCTC(1/5)
512 GTCTC
Bsp1286I 858 GTGCTC G[AGT]GC[ACT]^C
3685 GGGCAC
BspHI 1810 TCATGA T^CATGA
3916 TCATGA
BspMI 3744 ACCTGC ACCTGC(4/8)
604 GCAGGT
BsrDI 4109 CATTGC GCAATG(2/0)
84 GCAATG
BstEII 3277 GGTAACC G^GT[ACGT]ACC
3959 GGTAACC
BstPI 3277 GGTAACC G^GT[ACGT]ACC
3959 GGTAACC
EarI 2068 GAAGAG CTCTTC(1/4)
3920 GAAGAG
EcoO65I 3277 GGTAACC G^GT[ACGT]ACC
3959 GGTAACC
EcoT22I 520 ATGCAT ATGCA^T
2559 ATGCAT
Ksp632I 2068 GAAGAG CTCTTC(1/4)
3920 GAAGAG
MaeII 1723 ACGT A^CGT
2046 ACGT
MspA1I 613 CAGCTG C[AC]G^C[GT]G
1228 CAGCTG
NsiI 520 ATGCAT ATGCA^T
2559 ATGCAT
PvuII 613 CAGCTG CAG^CTG
1228 CAGCTG
RcaI 1810 TCATGA T^CATGA
3916 TCATGA
SfcI 175 CTATAG C^T[GA][CT]AG
3096 CTACAG
SspI 749 AATATT AAT^ATT
3738 AATATT
StuI 1516 AGGCCT AGG^CCT
3495 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
ApoI BalI BbsI BbvI BclI
BfaI BpuAI BsaJI BsiYI BslI
BsmFI BsmI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI EaeI EcoRII FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MvaI MwoI NdeI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII