##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccII AclI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspEI AspI AviII
AvrII BamHI BbrPI BcgI BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BsiEI BsiWI BspDI BspLU11I BssHII
BssSI BstBI BstUI Bsu36I CelII
ClaI CpoI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRV FseI FspI HpaI
KspI MamI MluI MvnI NaeI
NcoI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PstI PvuI
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SmaI Sse8387I
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 672 GGTACC G^GTACC
AccIII 142 TCCGGA T^CCGGA
AflII 1129 CTTAAG C^TTAAG
Asp700I 499 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 672 GGTACC G^GTACC
AspHI 1894 GAGCTC G[AT]GC[AT]^C
BanII 1894 GAGCTC G[GA]GC[CT]^C
BbeI 186 GGCGCC GGCGC^C
BbsI 1186 GAAGAC GAAGAC(2/6)
BciVI 3699 GTATCC GTATCC(6/5)
BcnI 58 CCCGG CC^[GC]GG
BfrI 1129 CTTAAG C^TTAAG
BglI 154 GCCGAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1492 AGATCT A^GATCT
BpuAI 1186 GAAGAC GAAGAC(2/6)
BseAI 142 TCCGGA T^CCGGA
BsiHKAI 1894 GAGCTC G[AT]GC[AT]^C
BsmBI 543 CGTCTC CGTCTC(1/5)
BspEI 142 TCCGGA T^CCGGA
BspHI 1286 TCATGA T^CATGA
BsrDI 1966 CATTGC GCAATG(2/0)
Bst1107I 2989 GTATAC GTA^TAC
BstEII 2315 GGTCACC G^GT[ACGT]ACC
BstPI 2315 GGTCACC G^GT[ACGT]ACC
BstZ17I 2989 GTATAC GTA^TAC
DraI 3045 TTTAAA TTT^AAA
DraIII 2258 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 164 CCGTGG C^C[GA][CT]GG
EcoO65I 2315 GGTCACC G^GT[ACGT]ACC
EcoRI 3767 GAATTC G^AATTC
HaeII 186 GGCGCC [GA]GCGC^[CT]
HincII 2786 GTCAAC GT[CT]^[GA]AC
HindII 2786 GTCAAC GT[CT]^[GA]AC
KasI 186 GGCGCC G^GCGCC
KpnI 672 GGTACC GGTAC^C
MfeI 2090 CAATTG C^AATTG
MroI 142 TCCGGA T^CCGGA
MunI 2090 CAATTG C^AATTG
NarI 186 GGCGCC GG^CGCC
NciI 58 CCCGG CC^[GC]GG
NdeI 2984 CATATG CA^TATG
PaeR7I 3507 CTCGAG C^TCGAG
PshAI 1947 GACTACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1286 TCATGA T^CATGA
SacI 1894 GAGCTC GAGCT^C
SfoI 186 GGCGCC GGC^GCC
SnaBI 2834 TACGTA TAC^GTA
SpeI 2747 ACTAGT A^CTAGT
SphI 2967 GCATGC GCATG^C
StuI 47 AGGCCT AGG^CCT
XcmI 2112 CCAAAGTGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3507 CTCGAG C^TCGAG
XmnI 499 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AlwNI 383 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
1435 CAGCAGCTG
AseI 2741 ATTAAT AT^TAAT
4200 ATTAAT
AsnI 2741 ATTAAT AT^TAAT
4200 ATTAAT
AvaI 369 CCCGAG C^[CT]CG[GA]G
3507 CTCGAG
BalI 1748 TGGCCA TGG^CCA
3577 TGGCCA
BbvCI 3676 CCTCAGC CCTCAGC(-5/-2)
133 GCTGAGG
BclI 630 TGATCA T^GATCA
4246 TGATCA
BsaWI 142 TCCGGA [AT]^CCGG[AT]
455 TCCGGT
BseRI 1421 GAGGAG GAGGAG(10/8)
2932 GAGGAG
BsmFI 3738 GGGAC GGGAC(10/14)
519 GTCCC
BsoBI 369 CCCGAG C^[CT]CG[GA]G
3507 CTCGAG
Bsp1407I 1975 TGTACA T^GTACA
2278 TGTACA
BspMI 1303 ACCTGC ACCTGC(4/8)
1650 GCAGGT
BsrFI 18 GCCGGT [GA]^CCGG[CT]
80 GCCGGT
BsrGI 1975 TGTACA T^GTACA
2278 TGTACA
BstAPI 382 GCAGTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4172 GCAAGAAGTGC
BstXI 1400 CCACTCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3328 CCACCTTTTTGG
Cfr10I 18 GCCGGT [GA]^CCGG[CT]
80 GCCGGT
EcoNI 327 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
397 CCTTGATAAGG
EcoT14I 522 CCTTGG C^C[AT][AT]GG
3961 CCAAGG
EcoT22I 2522 ATGCAT ATGCA^T
2965 ATGCAT
FbaI 630 TGATCA T^GATCA
4246 TGATCA
HgaI 542 GCGTC GACGC(5/10)
721 GCGTC
MluNI 1748 TGGCCA TGG^CCA
3577 TGGCCA
MscI 1748 TGGCCA TGG^CCA
3577 TGGCCA
NheI 895 GCTAGC G^CTAGC
1327 GCTAGC
NsiI 2522 ATGCAT ATGCA^T
2965 ATGCAT
PflMI 676 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2889 CCAGTATTTGG
PshBI 2741 ATTAAT AT^TAAT
4200 ATTAAT
PvuII 1438 CAGCTG CAG^CTG
1736 CAGCTG
SexAI 250 ACCAGGT A^CC[AT]GGT
675 ACCAGGT
SspBI 1975 TGTACA T^GTACA
2278 TGTACA
StyI 522 CCTTGG C^C[AT][AT]GG
3961 CCAAGG
Van91I 676 CCAGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2889 CCAGTATTTGG
XbaI 2323 TCTAGA T^CTAGA
2908 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaII BanI
BbvI BfaI BmyI BsaAI BsaHI
BsaJI BsgI BsiYI BslI BsmAI
BsmI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII