################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AccIII AclI AflII AflIII AgeI Alw44I AlwNI ApaI ApaLI AscI AspI AviII BalI BfrI BglII BsaBI BseAI BsgI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BstAPI BstBI ClaI CpoI EagI EclXI Eco52I EcoRV FseI FspI MamI MluI MluNI MroI MscI NheI NotI NruI NspV PacI PflFI PflMI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SbfI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I Van91I XbaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 1839 GACGTC GACGT^C Acc65I 244 GGTACC G^GTACC AhdI 900 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 943 CCTGAGG CC^T[ACGT]AGG Aor51HI 207 AGCGCT AGC^GCT AseI 3585 ATTAAT AT^TAAT AsnI 3585 ATTAAT AT^TAAT Asp718I 244 GGTACC G^GTACC AspEI 900 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 3623 CCTAGG C^CTAGG BamHI 195 GGATCC G^GATCC BbrPI 736 CACGTG CAC^GTG BbsI 1213 GTCTTC GAAGAC(2/6) BbvCI 565 GCTGAGG CCTCAGC(-5/-2) BcgI 386 CGACGCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1536 TGATCA T^GATCA BlnI 3623 CCTAGG C^CTAGG BpuAI 1213 GTCTTC GAAGAC(2/6) BsaAI 736 CACGTG [CT]AC^GT[GA] BsaI 898 GAGACC GGTCTC(1/5) BsiEI 529 CGGTCG CG[GA][CT]^CG BsrDI 2706 CATTGC GCAATG(2/0) BsrFI 378 GCCGGC [GA]^CCGG[CT] BssHII 521 GCGCGC G^CGCGC BssSI 249 CACGAG CACGAG(-5/-1) Bst1107I 3775 GTATAC GTA^TAC BstXI 3273 CCATGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 3775 GTATAC GTA^TAC Bsu36I 943 CCTGAGG CC^T[ACGT]AGG Cfr10I 378 GCCGGC [GA]^CCGG[CT] DraIII 11 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 357 GACAACAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 821 TGGCCG [CT]^GGCC[GA] Eam1105I 900 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 207 AGCGCT AGC^GCT Eco81I 943 CCTGAGG CC^T[ACGT]AGG EcoNI 750 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1536 TGATCA T^GATCA HpaI 3884 GTTAAC GTT^AAC KpnI 244 GGTACC GGTAC^C MfeI 3317 CAATTG C^AATTG MunI 3317 CAATTG C^AATTG NaeI 378 GCCGGC GCC^GGC NdeI 2209 CATATG CA^TATG NgoMIV 378 GCCGGC G^CCGGC PaeR7I 201 CTCGAG C^TCGAG PmaCI 736 CACGTG CAC^GTG PmlI 736 CACGTG CAC^GTG PshBI 3585 ATTAAT AT^TAAT PstI 1805 CTGCAG CTGCA^G SalI 531 GTCGAC G^TCGAC SapI 973 GAAGAGC GCTCTTC(1/4) SfiI 282 GGCCCGCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 232 CCCGGG CCC^GGG StuI 306 AGGCCT AGG^CCT TfiI 1935 GAATC G^A[AT]TC XhoI 201 CTCGAG C^TCGAG XmaCI 232 CCCGGG C^CCGGG XmaI 232 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BbeI 254 GGCGCC GGCGC^C 460 GGCGCC BciVI 3801 GGATAC GTATCC(6/5) 723 GTATCC BfaI 3121 CTAG C^TAG 3624 CTAG BglI 283 GCCCGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 700 GCCTCCTGGGC BlpI 1940 GCTCAGC GC^T[ACGT]AGC 2444 GCTCAGC Bpu1102I 1940 GCTCAGC GC^T[ACGT]AGC 2444 GCTCAGC BsaWI 527 TCCGGT [AT]^CCGG[AT] 2714 ACCGGA CelII 1940 GCTCAGC GC^T[ACGT]AGC 2444 GCTCAGC DraI 2239 TTTAAA TTT^AAA 3902 TTTAAA EcoT22I 2218 ATGCAT ATGCA^T 2890 ATGCAT HindIII 2261 AAGCTT A^AGCTT 3171 AAGCTT KasI 254 GGCGCC G^GCGCC 460 GGCGCC MaeI 3121 CTAG C^TAG 3624 CTAG NarI 254 GGCGCC GG^CGCC 460 GGCGCC NsiI 2218 ATGCAT ATGCA^T 2890 ATGCAT NspI 997 GCATGT [GA]CATG^[CT] 1347 ACATGC ScaI 1230 AGTACT AGT^ACT 3406 AGTACT SfoI 254 GGCGCC GGC^GCC 460 GGCGCC SmlI 201 CTCGAG C^T[CT][GA]AG 3265 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoO65I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI