##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BbeI BbrPI BcgI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaHI BseAI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MluI
MluNI MroI MscI NaeI NarI
NgoMIV NotI NruI PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SmlI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 858 CGCG CG^CG
Alw44I 719 GTGCAC G^TGCAC
ApaLI 719 GTGCAC G^TGCAC
Asp700I 2729 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 1180 GAAGAC GAAGAC(2/6)
BbvCI 1789 GCTGAGG CCTCAGC(-5/-2)
BcnI 233 CCCGG CC^[GC]GG
BpuAI 1180 GAAGAC GAAGAC(2/6)
BsaAI 3280 TACGTA [CT]AC^GT[GA]
BsaBI 156 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 634 GAGACC GGTCTC(1/5)
BsaWI 1091 ACCGGA [AT]^CCGG[AT]
BsmBI 288 CGTCTC CGTCTC(1/5)
BspHI 152 TCATGA T^CATGA
BspMI 2740 ACCTGC ACCTGC(4/8)
BsrDI 40 GCAATG GCAATG(2/0)
BstAPI 650 GCATGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1166 TTCGAA TT^CGAA
BstUI 858 CGCG CG^CG
BstXI 182 CCATGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1709 GACCTCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 441 TGGCCG [CT]^GGCC[GA]
EcoNI 909 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2733 GAATTC G^AATTC
EcoRV 142 GATATC GAT^ATC
HincII 1623 GTCAAC GT[CT]^[GA]AC
HindII 1623 GTCAAC GT[CT]^[GA]AC
HindIII 2581 AAGCTT A^AGCTT
MamI 156 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1588 CAATTG C^AATTG
MunI 1588 CAATTG C^AATTG
MvnI 858 CGCG CG^CG
NciI 233 CCCGG CC^[GC]GG
NdeI 2827 CATATG CA^TATG
NheI 699 GCTAGC G^CTAGC
NspV 1166 TTCGAA TT^CGAA
PstI 2401 CTGCAG CTGCA^G
RcaI 152 TCATGA T^CATGA
SacI 2292 GAGCTC GAGCT^C
SapI 540 GCTCTTC GCTCTTC(1/4)
ScaI 3726 AGTACT AGT^ACT
SfuI 1166 TTCGAA TT^CGAA
SnaBI 3280 TACGTA TAC^GTA
SphI 3488 GCATGC GCATG^C
TaqI 1167 TCGA T^CGA
TthHB8I 1167 TCGA T^CGA
XmnI 2729 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AseI 3639 ATTAAT AT^TAAT
3999 ATTAAT
AsnI 3639 ATTAAT AT^TAAT
3999 ATTAAT
AspHI 719 GTGCAC G[AT]GC[AT]^C
2292 GAGCTC
AvaII 1644 GGTCC G^G[AT]CC
1949 GGTCC
BanII 904 GAGCCC G[GA]GC[CT]^C
2292 GAGCTC
BciVI 2863 GGATAC GTATCC(6/5)
2598 GTATCC
BsgI 3316 CTGCAC GTGCAG(16/14)
899 GTGCAG
BsiHKAI 719 GTGCAC G[AT]GC[AT]^C
2292 GAGCTC
Bsp1407I 851 TGTACA T^GTACA
2974 TGTACA
BsrGI 851 TGTACA T^GTACA
2974 TGTACA
BstEII 177 GGTCACC G^GT[ACGT]ACC
1087 GGTGACC
BstPI 177 GGTCACC G^GT[ACGT]ACC
1087 GGTGACC
Cfr13I 1644 GGTCC G^G[ACGT]CC
1949 GGTCC
EarI 541 CTCTTC CTCTTC(1/4)
1025 CTCTTC
EcoO65I 177 GGTCACC G^GT[ACGT]ACC
1087 GGTGACC
HapII 234 CCGG C^CGG
1092 CCGG
HpaII 234 CCGG C^CGG
1092 CCGG
Ksp632I 541 CTCTTC CTCTTC(1/4)
1025 CTCTTC
MspA1I 1007 CAGCTG C[AC]G^C[GT]G
1778 CAGCTG
MspI 234 CCGG C^CGG
1092 CCGG
PflMI 102 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
787 CCATCTGTTGG
PshBI 3639 ATTAAT AT^TAAT
3999 ATTAAT
PvuII 1007 CAGCTG CAG^CTG
1778 CAGCTG
Sau96I 1644 GGTCC G^G[ACGT]CC
1949 GGTCC
SpeI 3291 ACTAGT A^CTAGT
3704 ACTAGT
SspBI 851 TGTACA T^GTACA
2974 TGTACA
Van91I 102 CCAAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
787 CCATCTGTTGG
XcmI 2133 CCATTTTCTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3363 CCATGAACAAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI BamHI BbvI
BclI BfaI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BsrI BssKI Bst1107I
BstF5I BstNI BstYI BstZ17I Cac8I
DdeI DpnII DraI DsaI EcoRII
EcoT14I EcoT22I FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI RsaI Sau3AI ScrFI SfaNI
SfcI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XbaI
XhoII