##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        Asp718I     
AspEI       AspHI       AvaI        AvaII       AviII       
BbeI        BbrPI       BbvCI       BfrI        BglI        
BsaHI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsoBI       BspDI       BspEI       BspMI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstUI       BstXI       BstZ17I     Cfr10I      
ClaI        CpoI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoRV       FseI        
FspI        HaeII       Hin1I       HpaI        KasI        
KpnI        KspI        MluI        MroI        MvnI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SexAI       SfiI        SfoI        SgrAI       
SmaI        SnaBI       StuI        SwaI        Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccI         4569  GTAGAC               GT^[AC][GT]AC
AlwNI        1931  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      1216  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         4572  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        4059  CCTAGG               C^CTAGG
BanI          987  GGTGCC               G^G[CT][GA]CC
BanII        1195  GGGCTC               G[GA]GC[CT]^C
BcgI         1017  GCAGCTCTATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1326  GTATCC               GTATCC(6/5)
BcnI         1542  CCCGG                CC^[GC]GG
BglII        3208  AGATCT               A^GATCT
BlnI         4059  CCTAGG               C^CTAGG
BlpI           10  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       10  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1151  TACGTG               [CT]AC^GT[GA]
BsaBI         751  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          793  GGTCTC               GGTCTC(1/5)
BsmBI          61  GAGACG               CGTCTC(1/5)
BspHI        2149  TCATGA               T^CATGA
BstBI        1715  TTCGAA               TT^CGAA
BstEII       1821  GGTTACC              G^GT[ACGT]ACC
BstPI        1821  GGTTACC              G^GT[ACGT]ACC
CelII          10  GCTCAGC              GC^T[ACGT]AGC
DraII        4055  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I     4055  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1821  GGTTACC              G^GT[ACGT]ACC
EcoRI         906  GAATTC               G^AATTC
HapII        1543  CCGG                 C^CGG
HgaI           47  GACGC                GACGC(5/10)
HpaII        1543  CCGG                 C^CGG
MamI          751  GATGATAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1568  CAATTG               C^AATTG
MspI         1543  CCGG                 C^CGG
MunI         1568  CAATTG               C^AATTG
NciI         1542  CCCGG                CC^[GC]GG
NcoI         1646  CCATGG               C^CATGG
NspV         1715  TTCGAA               TT^CGAA
PflFI        4572  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         2149  TCATGA               T^CATGA
SapI         1875  GCTCTTC              GCTCTTC(1/4)
SbfI         4289  CCTGCAGG             CCTGCA^GG
ScaI         1592  AGTACT               AGT^ACT
SfuI         1715  TTCGAA               TT^CGAA
Sse8387I     4289  CCTGCAGG             CCTGCA^GG
SspI         2421  AATATT               AAT^ATT
Tth111I      4572  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI          819  TCTAGA               T^CTAGA
XcmI         1778  CCAATTTACCTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         1216  GAAAAGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflIII       3486  ACATGT               A^C[GA][CT]GT
             4276  ACATGT              
BalI         1345  TGGCCA               TGG^CCA
             3453  TGGCCA              
BamHI        4366  GGATCC               G^GATCC
             4458  GGATCC              
BbsI          144  GTCTTC               GAAGAC(2/6)
             4671  GTCTTC              
BpuAI         144  GTCTTC               GAAGAC(2/6)
             4671  GTCTTC              
BsgI          920  CTGCAC               GTGCAG(16/14)
             1706  CTGCAC              
BsmI          649  GCATTC               GAATGC(1/-1)
             2520  GCATTC              
BspLU11I     3486  ACATGT               A^CATGT
             4276  ACATGT              
CfoI          212  GCGC                 GCG^C
             3844  GCGC                
DrdI          613  GACAACTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              885  GACAGAGCTGTC        
DsaI           26  CCGTGG               C^C[GA][CT]GG
             1646  CCATGG              
EaeI         1345  TGGCCA               [CT]^GGCC[GA]
             3453  TGGCCA              
EcoNI        1330  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2198  CCTAAGGGAGG         
EcoT22I      1635  ATGCAT               ATGCA^T
             3095  ATGCAT              
FauI          319  GCGGG                CCCGC(4/6)
             1130  GCGGG               
HhaI          212  GCGC                 GCG^C
             3844  GCGC                
HinP1I        212  GCGC                 G^CGC
             3844  GCGC                
HincII       1183  GTCAAC               GT[CT]^[GA]AC
             2473  GTTGAC              
HindII       1183  GTCAAC               GT[CT]^[GA]AC
             2473  GTTGAC              
HindIII       955  AAGCTT               A^AGCTT
             2858  AAGCTT              
MluNI        1345  TGGCCA               TGG^CCA
             3453  TGGCCA              
MscI         1345  TGGCCA               TGG^CCA
             3453  TGGCCA              
NdeI         2525  CATATG               CA^TATG
             4025  CATATG              
NsiI         1635  ATGCAT               ATGCA^T
             3095  ATGCAT              
SpeI          799  ACTAGT               A^CTAGT
             4486  ACTAGT              
SphI         4206  GCATGC               GCATG^C
             4300  GCATGC              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AocI        ApoI        AseI        AsnI        BbvI        
BclI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    Bsp1407I    BsrDI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EarI        Eco81I      EcoRII      EcoT14I     FbaI        
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PshBI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspBI       
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII