################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AvaI AvaII AviII BbeI BbrPI BbvCI BfrI BglI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI FspI HaeII Hin1I HpaI KasI KpnI KspI MluI MroI MvnI NaeI NarI NgoMIV NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SexAI SfiI SfoI SgrAI SmaI SnaBI StuI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 4569 GTAGAC GT^[AC][GT]AC AlwNI 1931 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1216 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 4572 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 4059 CCTAGG C^CTAGG BanI 987 GGTGCC G^G[CT][GA]CC BanII 1195 GGGCTC G[GA]GC[CT]^C BcgI 1017 GCAGCTCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1326 GTATCC GTATCC(6/5) BcnI 1542 CCCGG CC^[GC]GG BglII 3208 AGATCT A^GATCT BlnI 4059 CCTAGG C^CTAGG BlpI 10 GCTCAGC GC^T[ACGT]AGC Bpu1102I 10 GCTCAGC GC^T[ACGT]AGC BsaAI 1151 TACGTG [CT]AC^GT[GA] BsaBI 751 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 793 GGTCTC GGTCTC(1/5) BsmBI 61 GAGACG CGTCTC(1/5) BspHI 2149 TCATGA T^CATGA BstBI 1715 TTCGAA TT^CGAA BstEII 1821 GGTTACC G^GT[ACGT]ACC BstPI 1821 GGTTACC G^GT[ACGT]ACC CelII 10 GCTCAGC GC^T[ACGT]AGC DraII 4055 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO109I 4055 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 1821 GGTTACC G^GT[ACGT]ACC EcoRI 906 GAATTC G^AATTC HapII 1543 CCGG C^CGG HgaI 47 GACGC GACGC(5/10) HpaII 1543 CCGG C^CGG MamI 751 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1568 CAATTG C^AATTG MspI 1543 CCGG C^CGG MunI 1568 CAATTG C^AATTG NciI 1542 CCCGG CC^[GC]GG NcoI 1646 CCATGG C^CATGG NspV 1715 TTCGAA TT^CGAA PflFI 4572 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 2149 TCATGA T^CATGA SapI 1875 GCTCTTC GCTCTTC(1/4) SbfI 4289 CCTGCAGG CCTGCA^GG ScaI 1592 AGTACT AGT^ACT SfuI 1715 TTCGAA TT^CGAA Sse8387I 4289 CCTGCAGG CCTGCA^GG SspI 2421 AATATT AAT^ATT Tth111I 4572 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 819 TCTAGA T^CTAGA XcmI 1778 CCAATTTACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1216 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 3486 ACATGT A^C[GA][CT]GT 4276 ACATGT BalI 1345 TGGCCA TGG^CCA 3453 TGGCCA BamHI 4366 GGATCC G^GATCC 4458 GGATCC BbsI 144 GTCTTC GAAGAC(2/6) 4671 GTCTTC BpuAI 144 GTCTTC GAAGAC(2/6) 4671 GTCTTC BsgI 920 CTGCAC GTGCAG(16/14) 1706 CTGCAC BsmI 649 GCATTC GAATGC(1/-1) 2520 GCATTC BspLU11I 3486 ACATGT A^CATGT 4276 ACATGT CfoI 212 GCGC GCG^C 3844 GCGC DrdI 613 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 885 GACAGAGCTGTC DsaI 26 CCGTGG C^C[GA][CT]GG 1646 CCATGG EaeI 1345 TGGCCA [CT]^GGCC[GA] 3453 TGGCCA EcoNI 1330 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2198 CCTAAGGGAGG EcoT22I 1635 ATGCAT ATGCA^T 3095 ATGCAT FauI 319 GCGGG CCCGC(4/6) 1130 GCGGG HhaI 212 GCGC GCG^C 3844 GCGC HinP1I 212 GCGC G^CGC 3844 GCGC HincII 1183 GTCAAC GT[CT]^[GA]AC 2473 GTTGAC HindII 1183 GTCAAC GT[CT]^[GA]AC 2473 GTTGAC HindIII 955 AAGCTT A^AGCTT 2858 AAGCTT MluNI 1345 TGGCCA TGG^CCA 3453 TGGCCA MscI 1345 TGGCCA TGG^CCA 3453 TGGCCA NdeI 2525 CATATG CA^TATG 4025 CATATG NsiI 1635 ATGCAT ATGCA^T 3095 ATGCAT SpeI 799 ACTAGT A^CTAGT 4486 ACTAGT SphI 4206 GCATGC GCATG^C 4300 GCATGC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AocI ApoI AseI AsnI BbvI BclI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr13I DdeI DpnII DraI EarI Eco81I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII