##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccIII AclI AflII AscI AseI
AsnI Asp700I AviII BciVI BclI
BfrI BsaBI BseAI BspDI BspEI
BspHI BssHII BstBI ClaI DraI
DraIII EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI SapI SbfI
ScaI SfiI SfuI SnaBI SpeI
SphI Sse8387I SwaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 2950 GGTACC G^GTACC
AhdI 3191 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 2950 GGTACC G^GTACC
AspEI 3191 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 3964 CCTAGG C^CTAGG
BglII 1618 AGATCT A^GATCT
BlnI 3964 CCTAGG C^CTAGG
BsiWI 292 CGTACG C^GTACG
BsmBI 3534 GAGACG CGTCTC(1/5)
BsmI 1190 GAATGC GAATGC(1/-1)
Bsp1407I 1924 TGTACA T^GTACA
BspLU11I 2410 ACATGT A^CATGT
BsrBI 1935 GAGCGG CCGCTC(-3/-3)
BsrGI 1924 TGTACA T^GTACA
Bst1107I 2250 GTATAC GTA^TAC
BstEII 2636 GGTGACC G^GT[ACGT]ACC
BstPI 2636 GGTGACC G^GT[ACGT]ACC
BstXI 3931 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2250 GTATAC GTA^TAC
CpoI 2401 CGGACCG CG^G[AT]CCG
DrdI 27 GACAACCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3191 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2636 GGTGACC G^GT[ACGT]ACC
EcoRI 338 GAATTC G^AATTC
HincII 828 GTCAAC GT[CT]^[GA]AC
HindII 828 GTCAAC GT[CT]^[GA]AC
KpnI 2950 GGTACC GGTAC^C
MseI 3877 TTAA T^TAA
PpuMI 573 GGGTCCT [GA]G^G[AT]CC[CT]
RsrII 2401 CGGACCG CG^G[AT]CCG
SgrAI 3206 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1350 CTCAAG C^T[CT][GA]AG
SspBI 1924 TGTACA T^GTACA
SspI 1341 AATATT AAT^ATT
Tru9I 3877 TTAA T^TAA
XbaI 1175 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AatII 549 GACGTC GACGT^C
3005 GACGTC
AccI 36 GTCTAC GT^[AC][GT]AC
2250 GTATAC
AcsI 254 GAATTT [GA]^AATT[CT]
338 GAATTC
AgeI 1021 ACCGGT A^CCGGT
2404 ACCGGT
Alw44I 2441 GTGCAC G^TGCAC
3451 GTGCAC
AocI 1210 CCTCAGG CC^T[ACGT]AGG
2675 CCTCAGG
Aor51HI 100 AGCGCT AGC^GCT
2356 AGCGCT
ApaLI 2441 GTGCAC G^TGCAC
3451 GTGCAC
ApoI 254 GAATTT [GA]^AATT[CT]
338 GAATTC
AspI 1794 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3135 GACCAGGTC
BamHI 364 GGATCC G^GATCC
2913 GGATCC
BbeI 352 GGCGCC GGCGC^C
3906 GGCGCC
BbrPI 3093 CACGTG CAC^GTG
3573 CACGTG
BbsI 983 GAAGAC GAAGAC(2/6)
1706 GAAGAC
BlpI 2009 GCTCAGC GC^T[ACGT]AGC
3427 GCTGAGC
Bpu1102I 2009 GCTCAGC GC^T[ACGT]AGC
3427 GCTGAGC
BpuAI 983 GAAGAC GAAGAC(2/6)
1706 GAAGAC
BsrDI 17 CATTGC GCAATG(2/0)
3464 GCAATG
BstAPI 2135 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3857 GCAGGACTTGC
Bsu36I 1210 CCTCAGG CC^T[ACGT]AGG
2675 CCTCAGG
CelII 2009 GCTCAGC GC^T[ACGT]AGC
3427 GCTGAGC
EarI 1815 CTCTTC CTCTTC(1/4)
820 GAAGAG
Eco47III 100 AGCGCT AGC^GCT
2356 AGCGCT
Eco81I 1210 CCTCAGG CC^T[ACGT]AGG
2675 CCTCAGG
KasI 352 GGCGCC G^GCGCC
3906 GGCGCC
Ksp632I 1815 CTCTTC CTCTTC(1/4)
820 GAAGAG
KspI 2995 CCGCGG CCGC^GG
3558 CCGCGG
NarI 352 GGCGCC GG^CGCC
3906 GGCGCC
PflFI 1794 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3135 GACCAGGTC
PinAI 1021 ACCGGT A^CCGGT
2404 ACCGGT
PmaCI 3093 CACGTG CAC^GTG
3573 CACGTG
PmlI 3093 CACGTG CAC^GTG
3573 CACGTG
PvuII 620 CAGCTG CAG^CTG
3083 CAGCTG
SacII 2995 CCGCGG CCGC^GG
3558 CCGCGG
SfoI 352 GGCGCC GGC^GCC
3906 GGCGCC
Tth111I 1794 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
3135 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmaI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI