##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        Aor51HI     ApaI        AscI        Asp718I     
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BfrI        BglII       BlnI        BsaBI       
BsaWI       BseAI       BsiWI       BspDI       BspEI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     ClaI        CpoI        DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRV       FseI        FspI        HpaI        
KasI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        RsrII       SalI        SbfI        SexAI       
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcyI          201  GACGCC               G[GA]^CG[CT]C
AflIII       2323  ACATGT               A^C[GA][CT]GT
AhdI         2008  GACAATGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        645  GTGCAC               G^TGCAC
ApaLI         645  GTGCAC               G^TGCAC
AseI         1638  ATTAAT               AT^TAAT
AsnI         1638  ATTAAT               AT^TAAT
AspEI        2008  GACAATGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          459  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          389  GGTGCC               G^G[CT][GA]CC
BcgI          557  CGAGGCTGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1492  TGATCA               T^GATCA
BsaAI         792  TACGTG               [CT]AC^GT[GA]
BsaHI         201  GACGCC               G[GA]^CG[CT]C
BsgI          506  CTGCAC               GTGCAG(16/14)
BsiEI        1193  CGACCG               CG[GA][CT]^CG
BsmBI         120  GAGACG               CGTCTC(1/5)
Bsp1407I     2378  TGTACA               T^GTACA
BspHI        1838  TCATGA               T^CATGA
BspLU11I     2323  ACATGT               A^CATGT
BsrBI        1326  GAGCGG               CCGCTC(-3/-3)
BsrFI         158  GCCGGC               [GA]^CCGG[CT]
BsrGI        2378  TGTACA               T^GTACA
BssHII        215  GCGCGC               G^CGCGC
BstAPI         59  GCAGCCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        158  GCCGGC               [GA]^CCGG[CT]
DraI         1043  TTTAAA               TTT^AAA
Eam1105I     2008  GACAATGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      2768  ATGCAT               ATGCA^T
FbaI         1492  TGATCA               T^GATCA
Hin1I         201  GACGCC               G[GA]^CG[CT]C
HincII       2016  GTCAAC               GT[CT]^[GA]AC
HindII       2016  GTCAAC               GT[CT]^[GA]AC
KspI            9  CCGCGG               CCGC^GG
NaeI          158  GCCGGC               GCC^GGC
NgoMIV        158  GCCGGC               G^CCGGC
NsiI         2768  ATGCAT               ATGCA^T
PaeR7I         80  CTCGAG               C^TCGAG
PflFI         459  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        2958  CCAAGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        1638  ATTAAT               AT^TAAT
RcaI         1838  TCATGA               T^CATGA
SacII           9  CCGCGG               CCGC^GG
ScaI         1871  AGTACT               AGT^ACT
SfiI         1065  GGCCGGGCGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         3247  CCCGGG               CCC^GGG
SspBI        2378  TGTACA               T^GTACA
Tth111I       459  GACCGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       2958  CCAAGGGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1620  TCTAGA               T^CTAGA
XhoI           80  CTCGAG               C^TCGAG
XmaCI        3247  CCCGGG               C^CCGGG
XmaI         3247  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI          525  GTCTAC               GT^[AC][GT]AC
             2005  GTAGAC              
AlwI          305  GATCC                GGATC(4/5)
              533  GATCC               
Asp700I      2142  GAAGTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2887  GAAGAGTTTC          
BbsI         2926  GAAGAC               GAAGAC(2/6)
              880  GTCTTC              
BciVI        2341  GGATAC               GTATCC(6/5)
             2971  GTATCC              
BglI         1066  GCCGGGCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1799  GCCAATTTGGC         
BlpI          681  GCTCAGC              GC^T[ACGT]AGC
             1474  GCTGAGC             
Bpu1102I      681  GCTCAGC              GC^T[ACGT]AGC
             1474  GCTGAGC             
BpuAI        2926  GAAGAC               GAAGAC(2/6)
              880  GTCTTC              
BsrDI        2157  CATTGC               GCAATG(2/0)
             2700  CATTGC              
BssSI        1302  CACGAG               CACGAG(-5/-1)
              642  CTCGTG              
BstYI         304  AGATCC               [GA]^GATC[CT]
              532  AGATCC              
CelII         681  GCTCAGC              GC^T[ACGT]AGC
             1474  GCTGAGC             
EcoRI        1736  GAATTC               G^AATTC
             3276  GAATTC              
HaeII          35  AGCGCC               [GA]GCGC^[CT]
               84  AGCGCC              
HindIII      2847  AAGCTT               A^AGCTT
             3630  AAGCTT              
MflI          304  AGATCC               [GA]^GATC[CT]
              532  AGATCC              
NcoI           88  CCATGG               C^CATGG
             2237  CCATGG              
NspI         2313  GCATGT               [GA]CATG^[CT]
             2323  ACATGT              
PstI         1465  CTGCAG               CTGCA^G
             2421  CTGCAG              
XhoII         304  AGATCC               [GA]^GATC[CT]
              532  AGATCC              
XmnI         2142  GAAGTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2887  GAAGAGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwNI       AocI        ApoI        AspHI       AvaI        
AvaII       BalI        BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        PpuMI       PvuII       RsaI        
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI