################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Aor51HI ApaI AscI Asp718I AviII AvrII BamHI BbeI BbrPI BbvCI BfrI BglII BlnI BsaBI BsaWI BseAI BsiWI BspDI BspEI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FseI FspI HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcyI 201 GACGCC G[GA]^CG[CT]C AflIII 2323 ACATGT A^C[GA][CT]GT AhdI 2008 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 645 GTGCAC G^TGCAC ApaLI 645 GTGCAC G^TGCAC AseI 1638 ATTAAT AT^TAAT AsnI 1638 ATTAAT AT^TAAT AspEI 2008 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 459 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 389 GGTGCC G^G[CT][GA]CC BcgI 557 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1492 TGATCA T^GATCA BsaAI 792 TACGTG [CT]AC^GT[GA] BsaHI 201 GACGCC G[GA]^CG[CT]C BsgI 506 CTGCAC GTGCAG(16/14) BsiEI 1193 CGACCG CG[GA][CT]^CG BsmBI 120 GAGACG CGTCTC(1/5) Bsp1407I 2378 TGTACA T^GTACA BspHI 1838 TCATGA T^CATGA BspLU11I 2323 ACATGT A^CATGT BsrBI 1326 GAGCGG CCGCTC(-3/-3) BsrFI 158 GCCGGC [GA]^CCGG[CT] BsrGI 2378 TGTACA T^GTACA BssHII 215 GCGCGC G^CGCGC BstAPI 59 GCAGCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 158 GCCGGC [GA]^CCGG[CT] DraI 1043 TTTAAA TTT^AAA Eam1105I 2008 GACAATGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 2768 ATGCAT ATGCA^T FbaI 1492 TGATCA T^GATCA Hin1I 201 GACGCC G[GA]^CG[CT]C HincII 2016 GTCAAC GT[CT]^[GA]AC HindII 2016 GTCAAC GT[CT]^[GA]AC KspI 9 CCGCGG CCGC^GG NaeI 158 GCCGGC GCC^GGC NgoMIV 158 GCCGGC G^CCGGC NsiI 2768 ATGCAT ATGCA^T PaeR7I 80 CTCGAG C^TCGAG PflFI 459 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2958 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 1638 ATTAAT AT^TAAT RcaI 1838 TCATGA T^CATGA SacII 9 CCGCGG CCGC^GG ScaI 1871 AGTACT AGT^ACT SfiI 1065 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 3247 CCCGGG CCC^GGG SspBI 2378 TGTACA T^GTACA Tth111I 459 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2958 CCAAGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1620 TCTAGA T^CTAGA XhoI 80 CTCGAG C^TCGAG XmaCI 3247 CCCGGG C^CCGGG XmaI 3247 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 525 GTCTAC GT^[AC][GT]AC 2005 GTAGAC AlwI 305 GATCC GGATC(4/5) 533 GATCC Asp700I 2142 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2887 GAAGAGTTTC BbsI 2926 GAAGAC GAAGAC(2/6) 880 GTCTTC BciVI 2341 GGATAC GTATCC(6/5) 2971 GTATCC BglI 1066 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1799 GCCAATTTGGC BlpI 681 GCTCAGC GC^T[ACGT]AGC 1474 GCTGAGC Bpu1102I 681 GCTCAGC GC^T[ACGT]AGC 1474 GCTGAGC BpuAI 2926 GAAGAC GAAGAC(2/6) 880 GTCTTC BsrDI 2157 CATTGC GCAATG(2/0) 2700 CATTGC BssSI 1302 CACGAG CACGAG(-5/-1) 642 CTCGTG BstYI 304 AGATCC [GA]^GATC[CT] 532 AGATCC CelII 681 GCTCAGC GC^T[ACGT]AGC 1474 GCTGAGC EcoRI 1736 GAATTC G^AATTC 3276 GAATTC HaeII 35 AGCGCC [GA]GCGC^[CT] 84 AGCGCC HindIII 2847 AAGCTT A^AGCTT 3630 AAGCTT MflI 304 AGATCC [GA]^GATC[CT] 532 AGATCC NcoI 88 CCATGG C^CATGG 2237 CCATGG NspI 2313 GCATGT [GA]CATG^[CT] 2323 ACATGT PstI 1465 CTGCAG CTGCA^G 2421 CTGCAG XhoII 304 AGATCC [GA]^GATC[CT] 532 AGATCC XmnI 2142 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2887 GAAGAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI