##################################
# NO CUT ENZYMES (   94 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AflII       AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp700I     AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BcgI        
BclI        BfrI        BlnI        BsaWI       BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspHI       BsrBI       BsrFI       BssHII      
Bst1107I    BstUI       BstZ17I     Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      FauI        FbaI        FseI        
FspI        HaeII       KasI        KspI        MluI        
MroI        MspA1I      MvnI        NaeI        NarI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    StuI        
SwaI        Tth111I     XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

Acc65I       1050  GGTACC               G^GTACC
AciI         1868  GCGG                 CCGC(-3/-1)
AcyI         2550  GGCGTC               G[GA]^CG[CT]C
AhdI         2380  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         1438  GGATC                GGATC(4/5)
AlwNI        2536  CAGATTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          646  CCTGAGG              CC^T[ACGT]AGG
AseI         3716  ATTAAT               AT^TAAT
AsnI         3716  ATTAAT               AT^TAAT
Asp718I      1050  GGTACC               G^GTACC
AspEI        2380  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI          645  GCCTGAGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2807  AGATCT               A^GATCT
BlpI         2215  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2215  GCTGAGC              GC^T[ACGT]AGC
BsaHI        2550  GGCGTC               G[GA]^CG[CT]C
BsaI         3490  GGTCTC               GGTCTC(1/5)
BsmI         3242  GAATGC               GAATGC(1/-1)
Bsp1407I      997  TGTACA               T^GTACA
BsrGI         997  TGTACA               T^GTACA
BssSI        2896  CTCGTG               CACGAG(-5/-1)
BstAPI        733  GCAGCTTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1994  TTCGAA               TT^CGAA
BstEII       2827  GGTGACC              G^GT[ACGT]ACC
BstPI        2827  GGTGACC              G^GT[ACGT]ACC
Bsu36I        646  CCTGAGG              CC^T[ACGT]AGG
CelII        2215  GCTGAGC              GC^T[ACGT]AGC
CfoI         1788  GCGC                 GCG^C
DraII         877  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI          743  CCATGG               C^C[GA][CT]GG
Eam1105I     2380  GACAACTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        646  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      877  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      2827  GGTGACC              G^GT[ACGT]ACC
EcoRV        2708  GATATC               GAT^ATC
HapII        3233  CCGG                 C^CGG
HgaI         2551  GCGTC                GACGC(5/10)
HhaI         1788  GCGC                 GCG^C
Hin1I        2550  GGCGTC               G[GA]^CG[CT]C
HinP1I       1788  GCGC                 G^CGC
HindIII      1724  AAGCTT               A^AGCTT
HpaI         1276  GTTAAC               GTT^AAC
HpaII        3233  CCGG                 C^CGG
KpnI         1050  GGTACC               GGTAC^C
MfeI         1313  CAATTG               C^AATTG
MspI         3233  CCGG                 C^CGG
MunI         1313  CAATTG               C^AATTG
NcoI          743  CCATGG               C^CATGG
NdeI         2954  CATATG               CA^TATG
NspV         1994  TTCGAA               TT^CGAA
PshBI        3716  ATTAAT               AT^TAAT
SacI         3565  GAGCTC               GAGCT^C
SapI         1930  GCTCTTC              GCTCTTC(1/4)
SexAI        2930  ACCAGGT              A^CC[AT]GGT
SfuI         1994  TTCGAA               TT^CGAA
SmaI         3232  CCCGGG               CCC^GGG
SmlI          686  CTCAAG               C^T[CT][GA]AG
SspBI         997  TGTACA               T^GTACA
XbaI           24  TCTAGA               T^CTAGA
XcmI         3176  CCAGTTACACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        3232  CCCGGG               C^CCGGG
XmaI         3232  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AvaI           94  CCCGAG               C^[CT]CG[GA]G
             3232  CCCGGG              
AvaII         986  GGACC                G^G[AT]CC
             3208  GGACC               
BanII           9  GAGCCC               G[GA]GC[CT]^C
             3565  GAGCTC              
BciVI        3575  GGATAC               GTATCC(6/5)
             1941  GTATCC              
BcnI         3232  CCCGG                CC^[GC]GG
             3233  CCGGG               
BsaAI         667  TACGTG               [CT]AC^GT[GA]
             2671  TACGTG              
BsaBI        1137  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3576  GATACCCATC          
BsoBI          94  CCCGAG               C^[CT]CG[GA]G
             3232  CCCGGG              
BspLU11I     2053  ACATGT               A^CATGT
             2348  ACATGT              
BspMI         276  ACCTGC               ACCTGC(4/8)
             1236  GCAGGT              
BsrDI         500  CATTGC               GCAATG(2/0)
              440  GCAATG              
BstXI         939  CCAGAAGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3310  CCACCTGACTGG        
BstYI        1438  GGATCT               [GA]^GATC[CT]
             2807  AGATCT              
DraI         1443  TTTAAA               TTT^AAA
             3363  TTTAAA              
EarI         1931  CTCTTC               CTCTTC(1/4)
              370  GAAGAG              
EcoNI         957  CCTGATGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3250  CCTGCTGGAGG         
EcoRI         658  GAATTC               G^AATTC
             1997  GAATTC              
EcoT22I       497  ATGCAT               ATGCA^T
             1684  ATGCAT              
HincII        118  GTTGAC               GT[CT]^[GA]AC
             1276  GTTAAC              
HindII        118  GTTGAC               GT[CT]^[GA]AC
             1276  GTTAAC              
Ksp632I      1931  CTCTTC               CTCTTC(1/4)
              370  GAAGAG              
MamI         1137  GATTAAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3576  GATACCCATC          
MflI         1438  GGATCT               [GA]^GATC[CT]
             2807  AGATCT              
NciI         3232  CCCGG                CC^[GC]GG
             3233  CCGGG               
NsiI          497  ATGCAT               ATGCA^T
             1684  ATGCAT              
TaqI         1995  TCGA                 T^CGA
             3619  TCGA                
TthHB8I      1995  TCGA                 T^CGA
             3619  TCGA                
XhoII        1438  GGATCT               [GA]^GATC[CT]
             2807  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        ApoI        
AspHI       BalI        BanI        BbsI        BbvCI       
BbvI        BfaI        BmyI        BpmI        BpuAI       
BsaJI       BseRI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       Cac8I       Cfr13I      DdeI        
DpnII       EaeI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I