################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII AccI AccII AccIII AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BglI BlnI BpuAI BsaAI BsaHI BseAI BseRI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I FseI FspI HaeII HgaI Hin1I KasI KspI MluI MroI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 2067 GGTACC G^GTACC AclI 1900 AACGTT AA^CGTT AflII 1795 CTTAAG C^TTAAG AflIII 2927 ACATGT A^C[GA][CT]GT AlwNI 2104 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 2402 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2067 GGTACC G^GTACC AspHI 754 GAGCAC G[AT]GC[AT]^C AvaII 2735 GGTCC G^G[AT]CC BalI 828 TGGCCA TGG^CCA BamHI 3083 GGATCC G^GATCC BanI 2067 GGTACC G^G[CT][GA]CC BanII 1153 GAGCCC G[GA]GC[CT]^C BclI 2613 TGATCA T^GATCA BfrI 1795 CTTAAG C^TTAAG BlpI 154 GCTTAGC GC^T[ACGT]AGC BpmI 7 CTCCAG CTGGAG(16/14) Bpu1102I 154 GCTTAGC GC^T[ACGT]AGC BsaBI 1264 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3301 GAGACC GGTCTC(1/5) BsaJI 848 CCATGG C^C[ACGT][ACGT]GG BsgI 2384 GTGCAG GTGCAG(16/14) BsiHKAI 754 GAGCAC G[AT]GC[AT]^C BspLU11I 2927 ACATGT A^CATGT BstEII 2418 GGTAACC G^GT[ACGT]ACC BstPI 2418 GGTAACC G^GT[ACGT]ACC CelII 154 GCTTAGC GC^T[ACGT]AGC CfoI 2440 GCGC GCG^C Cfr13I 2735 GGTCC G^G[ACGT]CC DraIII 757 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 848 CCATGG C^C[GA][CT]GG EaeI 828 TGGCCA [CT]^GGCC[GA] EcoNI 1630 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2418 GGTAACC G^GT[ACGT]ACC EcoT14I 848 CCATGG C^C[AT][AT]GG EcoT22I 3487 ATGCAT ATGCA^T FauI 1929 GCGGG CCCGC(4/6) FbaI 2613 TGATCA T^GATCA HhaI 2440 GCGC GCG^C HinP1I 2440 GCGC G^CGC KpnI 2067 GGTACC GGTAC^C MamI 1264 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1982 CAATTG C^AATTG MluNI 828 TGGCCA TGG^CCA MscI 828 TGGCCA TGG^CCA MspA1I 2504 CAGCTG C[AC]G^C[GT]G MunI 1982 CAATTG C^AATTG NcoI 848 CCATGG C^CATGG NsiI 3487 ATGCAT ATGCA^T PleI 1016 GACTC GAGTC(4/5) PshAI 273 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1900 AACGTT AA^CGTT PvuII 2504 CAGCTG CAG^CTG Sau96I 2735 GGTCC G^G[ACGT]CC SpeI 1995 ACTAGT A^CTAGT StuI 705 AGGCCT AGG^CCT StyI 848 CCATGG C^C[AT][AT]GG SwaI 2352 ATTTAAAT ATTT^AAAT Tsp45I 1218 GTCAC ^GT[GC]AC XcmI 1337 CCATGCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2402 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 686 GCGG CCGC(-3/-1) 1929 GCGG AocI 1224 CCTAAGG CC^T[ACGT]AGG 3369 CCTGAGG BglII 599 AGATCT A^GATCT 659 AGATCT BsaWI 1953 TCCGGT [AT]^CCGG[AT] 3304 ACCGGA BspMI 864 ACCTGC ACCTGC(4/8) 1890 GCAGGT BssKI 800 CCAGG ^CC[ACGT]GG 922 CCTGG BstF5I 925 GGATG GGATG(2/0) 1528 GGATG BstNI 800 CCAGG CC^[AT]GG 922 CCTGG Bsu36I 1224 CCTAAGG CC^T[ACGT]AGG 3369 CCTGAGG Eco81I 1224 CCTAAGG CC^T[ACGT]AGG 3369 CCTGAGG EcoRI 1846 GAATTC G^AATTC 2571 GAATTC EcoRII 800 CCAGG ^CC[AT]GG 922 CCTGG EcoRV 900 GATATC GAT^ATC 2788 GATATC FokI 925 GGATG GGATG(9/13) 1528 GGATG HaeIII 706 GGCC GG^CC 829 GGCC HapII 1954 CCGG C^CGG 3305 CCGG HpaII 1954 CCGG C^CGG 3305 CCGG MspI 1954 CCGG C^CGG 3305 CCGG MvaI 800 CCAGG CC^[AT]GG 922 CCTGG PstI 883 CTGCAG CTGCA^G 2045 CTGCAG ScaI 211 AGTACT AGT^ACT 1728 AGTACT ScrFI 800 CCAGG CC^[ACGT]GG 922 CCTGG SmlI 543 CTCAAG C^T[CT][GA]AG 1795 CTTAAG SphI 1006 GCATGC GCATG^C 2634 GCATGC TaqI 2094 TCGA T^CGA 2475 TCGA TthHB8I 2094 TCGA T^CGA 2475 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbvI BfaI BmyI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BstYI Cac8I DdeI DpnII DraI EarI Fnu4HI HincII HindII HindIII HinfI HpaI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MwoI NdeII NlaIII NlaIV NspI RsaI Sau3AI SfaNI SfcI SspBI SspI TfiI Tru9I TseI Tsp509I TspRI XhoII