##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AcyI        
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspEI       
AspI        AvaI        AviII       AvrII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BglI        BlnI        BpuAI       BsaAI       
BsaHI       BseAI       BseRI       BsiEI       BsiWI       
BsmBI       BsoBI       BspDI       BspEI       BspHI       
BsrBI       BsrFI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstXI       BstZ17I     
Cfr10I      ClaI        CpoI        DraII       DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO109I    FseI        FspI        HaeII       HgaI        
Hin1I       KasI        KspI        MluI        MroI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
Sse8387I    Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I       2067  GGTACC               G^GTACC
AclI         1900  AACGTT               AA^CGTT
AflII        1795  CTTAAG               C^TTAAG
AflIII       2927  ACATGT               A^C[GA][CT]GT
AlwNI        2104  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I      2402  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2067  GGTACC               G^GTACC
AspHI         754  GAGCAC               G[AT]GC[AT]^C
AvaII        2735  GGTCC                G^G[AT]CC
BalI          828  TGGCCA               TGG^CCA
BamHI        3083  GGATCC               G^GATCC
BanI         2067  GGTACC               G^G[CT][GA]CC
BanII        1153  GAGCCC               G[GA]GC[CT]^C
BclI         2613  TGATCA               T^GATCA
BfrI         1795  CTTAAG               C^TTAAG
BlpI          154  GCTTAGC              GC^T[ACGT]AGC
BpmI            7  CTCCAG               CTGGAG(16/14)
Bpu1102I      154  GCTTAGC              GC^T[ACGT]AGC
BsaBI        1264  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         3301  GAGACC               GGTCTC(1/5)
BsaJI         848  CCATGG               C^C[ACGT][ACGT]GG
BsgI         2384  GTGCAG               GTGCAG(16/14)
BsiHKAI       754  GAGCAC               G[AT]GC[AT]^C
BspLU11I     2927  ACATGT               A^CATGT
BstEII       2418  GGTAACC              G^GT[ACGT]ACC
BstPI        2418  GGTAACC              G^GT[ACGT]ACC
CelII         154  GCTTAGC              GC^T[ACGT]AGC
CfoI         2440  GCGC                 GCG^C
Cfr13I       2735  GGTCC                G^G[ACGT]CC
DraIII        757  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          848  CCATGG               C^C[GA][CT]GG
EaeI          828  TGGCCA               [CT]^GGCC[GA]
EcoNI        1630  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2418  GGTAACC              G^GT[ACGT]ACC
EcoT14I       848  CCATGG               C^C[AT][AT]GG
EcoT22I      3487  ATGCAT               ATGCA^T
FauI         1929  GCGGG                CCCGC(4/6)
FbaI         2613  TGATCA               T^GATCA
HhaI         2440  GCGC                 GCG^C
HinP1I       2440  GCGC                 G^CGC
KpnI         2067  GGTACC               GGTAC^C
MamI         1264  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1982  CAATTG               C^AATTG
MluNI         828  TGGCCA               TGG^CCA
MscI          828  TGGCCA               TGG^CCA
MspA1I       2504  CAGCTG               C[AC]G^C[GT]G
MunI         1982  CAATTG               C^AATTG
NcoI          848  CCATGG               C^CATGG
NsiI         3487  ATGCAT               ATGCA^T
PleI         1016  GACTC                GAGTC(4/5)
PshAI         273  GACTACCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1900  AACGTT               AA^CGTT
PvuII        2504  CAGCTG               CAG^CTG
Sau96I       2735  GGTCC                G^G[ACGT]CC
SpeI         1995  ACTAGT               A^CTAGT
StuI          705  AGGCCT               AGG^CCT
StyI          848  CCATGG               C^C[AT][AT]GG
SwaI         2352  ATTTAAAT             ATTT^AAAT
Tsp45I       1218  GTCAC                ^GT[GC]AC
XcmI         1337  CCATGCAATTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2402  GAAAACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AciI          686  GCGG                 CCGC(-3/-1)
             1929  GCGG                
AocI         1224  CCTAAGG              CC^T[ACGT]AGG
             3369  CCTGAGG             
BglII         599  AGATCT               A^GATCT
              659  AGATCT              
BsaWI        1953  TCCGGT               [AT]^CCGG[AT]
             3304  ACCGGA              
BspMI         864  ACCTGC               ACCTGC(4/8)
             1890  GCAGGT              
BssKI         800  CCAGG                ^CC[ACGT]GG
              922  CCTGG               
BstF5I        925  GGATG                GGATG(2/0)
             1528  GGATG               
BstNI         800  CCAGG                CC^[AT]GG
              922  CCTGG               
Bsu36I       1224  CCTAAGG              CC^T[ACGT]AGG
             3369  CCTGAGG             
Eco81I       1224  CCTAAGG              CC^T[ACGT]AGG
             3369  CCTGAGG             
EcoRI        1846  GAATTC               G^AATTC
             2571  GAATTC              
EcoRII        800  CCAGG                ^CC[AT]GG
              922  CCTGG               
EcoRV         900  GATATC               GAT^ATC
             2788  GATATC              
FokI          925  GGATG                GGATG(9/13)
             1528  GGATG               
HaeIII        706  GGCC                 GG^CC
              829  GGCC                
HapII        1954  CCGG                 C^CGG
             3305  CCGG                
HpaII        1954  CCGG                 C^CGG
             3305  CCGG                
MspI         1954  CCGG                 C^CGG
             3305  CCGG                
MvaI          800  CCAGG                CC^[AT]GG
              922  CCTGG               
PstI          883  CTGCAG               CTGCA^G
             2045  CTGCAG              
ScaI          211  AGTACT               AGT^ACT
             1728  AGTACT              
ScrFI         800  CCAGG                CC^[ACGT]GG
              922  CCTGG               
SmlI          543  CTCAAG               C^T[CT][GA]AG
             1795  CTTAAG              
SphI         1006  GCATGC               GCATG^C
             2634  GCATGC              
TaqI         2094  TCGA                 T^CGA
             2475  TCGA                
TthHB8I      2094  TCGA                 T^CGA
             2475  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
BbvI        BfaI        BmyI        BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    Bsp1407I    
BsrDI       BsrGI       BsrI        BstYI       Cac8I       
DdeI        DpnII       DraI        EarI        Fnu4HI      
HincII      HindII      HindIII     HinfI       HpaI        
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MwoI        NdeII       NlaIII      
NlaIV       NspI        RsaI        Sau3AI      SfaNI       
SfcI        SspBI       SspI        TfiI        Tru9I       
TseI        Tsp509I     TspRI       XhoII