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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccI AccII AccIII AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BglI BlnI BpuAI BsaAI
BsaHI BseAI BseRI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO109I FseI FspI HaeII HgaI
Hin1I KasI KspI MluI MroI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshBI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 2067 GGTACC G^GTACC
AclI 1900 AACGTT AA^CGTT
AflII 1795 CTTAAG C^TTAAG
AflIII 2927 ACATGT A^C[GA][CT]GT
AlwNI 2104 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 2402 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2067 GGTACC G^GTACC
AspHI 754 GAGCAC G[AT]GC[AT]^C
AvaII 2735 GGTCC G^G[AT]CC
BalI 828 TGGCCA TGG^CCA
BamHI 3083 GGATCC G^GATCC
BanI 2067 GGTACC G^G[CT][GA]CC
BanII 1153 GAGCCC G[GA]GC[CT]^C
BclI 2613 TGATCA T^GATCA
BfrI 1795 CTTAAG C^TTAAG
BlpI 154 GCTTAGC GC^T[ACGT]AGC
BpmI 7 CTCCAG CTGGAG(16/14)
Bpu1102I 154 GCTTAGC GC^T[ACGT]AGC
BsaBI 1264 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3301 GAGACC GGTCTC(1/5)
BsaJI 848 CCATGG C^C[ACGT][ACGT]GG
BsgI 2384 GTGCAG GTGCAG(16/14)
BsiHKAI 754 GAGCAC G[AT]GC[AT]^C
BspLU11I 2927 ACATGT A^CATGT
BstEII 2418 GGTAACC G^GT[ACGT]ACC
BstPI 2418 GGTAACC G^GT[ACGT]ACC
CelII 154 GCTTAGC GC^T[ACGT]AGC
CfoI 2440 GCGC GCG^C
Cfr13I 2735 GGTCC G^G[ACGT]CC
DraIII 757 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 848 CCATGG C^C[GA][CT]GG
EaeI 828 TGGCCA [CT]^GGCC[GA]
EcoNI 1630 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2418 GGTAACC G^GT[ACGT]ACC
EcoT14I 848 CCATGG C^C[AT][AT]GG
EcoT22I 3487 ATGCAT ATGCA^T
FauI 1929 GCGGG CCCGC(4/6)
FbaI 2613 TGATCA T^GATCA
HhaI 2440 GCGC GCG^C
HinP1I 2440 GCGC G^CGC
KpnI 2067 GGTACC GGTAC^C
MamI 1264 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1982 CAATTG C^AATTG
MluNI 828 TGGCCA TGG^CCA
MscI 828 TGGCCA TGG^CCA
MspA1I 2504 CAGCTG C[AC]G^C[GT]G
MunI 1982 CAATTG C^AATTG
NcoI 848 CCATGG C^CATGG
NsiI 3487 ATGCAT ATGCA^T
PleI 1016 GACTC GAGTC(4/5)
PshAI 273 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1900 AACGTT AA^CGTT
PvuII 2504 CAGCTG CAG^CTG
Sau96I 2735 GGTCC G^G[ACGT]CC
SpeI 1995 ACTAGT A^CTAGT
StuI 705 AGGCCT AGG^CCT
StyI 848 CCATGG C^C[AT][AT]GG
SwaI 2352 ATTTAAAT ATTT^AAAT
Tsp45I 1218 GTCAC ^GT[GC]AC
XcmI 1337 CCATGCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2402 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 686 GCGG CCGC(-3/-1)
1929 GCGG
AocI 1224 CCTAAGG CC^T[ACGT]AGG
3369 CCTGAGG
BglII 599 AGATCT A^GATCT
659 AGATCT
BsaWI 1953 TCCGGT [AT]^CCGG[AT]
3304 ACCGGA
BspMI 864 ACCTGC ACCTGC(4/8)
1890 GCAGGT
BssKI 800 CCAGG ^CC[ACGT]GG
922 CCTGG
BstF5I 925 GGATG GGATG(2/0)
1528 GGATG
BstNI 800 CCAGG CC^[AT]GG
922 CCTGG
Bsu36I 1224 CCTAAGG CC^T[ACGT]AGG
3369 CCTGAGG
Eco81I 1224 CCTAAGG CC^T[ACGT]AGG
3369 CCTGAGG
EcoRI 1846 GAATTC G^AATTC
2571 GAATTC
EcoRII 800 CCAGG ^CC[AT]GG
922 CCTGG
EcoRV 900 GATATC GAT^ATC
2788 GATATC
FokI 925 GGATG GGATG(9/13)
1528 GGATG
HaeIII 706 GGCC GG^CC
829 GGCC
HapII 1954 CCGG C^CGG
3305 CCGG
HpaII 1954 CCGG C^CGG
3305 CCGG
MspI 1954 CCGG C^CGG
3305 CCGG
MvaI 800 CCAGG CC^[AT]GG
922 CCTGG
PstI 883 CTGCAG CTGCA^G
2045 CTGCAG
ScaI 211 AGTACT AGT^ACT
1728 AGTACT
ScrFI 800 CCAGG CC^[ACGT]GG
922 CCTGG
SmlI 543 CTCAAG C^T[CT][GA]AG
1795 CTTAAG
SphI 1006 GCATGC GCATG^C
2634 GCATGC
TaqI 2094 TCGA T^CGA
2475 TCGA
TthHB8I 2094 TCGA T^CGA
2475 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BfaI BmyI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BsrDI BsrGI BsrI BstYI Cac8I
DdeI DpnII DraI EarI Fnu4HI
HincII HindII HindIII HinfI HpaI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI SfaNI
SfcI SspBI SspI TfiI Tru9I
TseI Tsp509I TspRI XhoII