##################################
# NO CUT ENZYMES (  103 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AflII       
AgeI        AhdI        Aor51HI     ApaI        AscI        
AseI        AsnI        AspEI       AspI        AviII       
BbeI        BbrPI       BbsI        BciVI       BclI        
BfrI        BglI        BglII       BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BseAI       
BsiWI       BspDI       BspEI       BsrDI       BssHII      
BssSI       BstUI       CelII       CfoI        ClaI        
CpoI        DraII       DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO109I    
FauI        FbaI        FseI        FspI        HaeII       
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        KasI        KspI        MamI        MluI        
MroI        MvnI        NaeI        NarI        NcoI        
NgoMIV      NheI        NotI        NruI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmlI        PpuMI       PshAI       PshBI       PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        Tth111I     
Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

Acc65I       2879  GGTACC               G^GTACC
AclI         1470  AACGTT               AA^CGTT
AflIII       2203  ACATGT               A^C[GA][CT]GT
Asp718I      2879  GGTACC               G^GTACC
AvrII        1862  CCTAGG               C^CTAGG
BamHI        1050  GGATCC               G^GATCC
BlnI         1862  CCTAGG               C^CTAGG
BsiEI        3698  CGGTCG               CG[GA][CT]^CG
BsmBI        1714  GAGACG               CGTCTC(1/5)
BsmFI         874  GGGAC                GGGAC(10/14)
Bsp1407I     2016  TGTACA               T^GTACA
BspHI         633  TCATGA               T^CATGA
BspLU11I     2203  ACATGT               A^CATGT
BspMI        1029  ACCTGC               ACCTGC(4/8)
BsrBI        3566  GAGCGG               CCGCTC(-3/-3)
BsrGI        2016  TGTACA               T^GTACA
BstBI        3049  TTCGAA               TT^CGAA
BstEII        479  GGTGACC              G^GT[ACGT]ACC
BstPI         479  GGTGACC              G^GT[ACGT]ACC
EcoNI        2127  CCTTTATTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       479  GGTGACC              G^GT[ACGT]ACC
EcoT22I      2751  ATGCAT               ATGCA^T
HgaI          350  GACGC                GACGC(5/10)
HindIII      1755  AAGCTT               A^AGCTT
KpnI         2879  GGTACC               GGTAC^C
MfeI         3452  CAATTG               C^AATTG
MunI         3452  CAATTG               C^AATTG
NdeI         3430  CATATG               CA^TATG
NsiI         2751  ATGCAT               ATGCA^T
NspV         3049  TTCGAA               TT^CGAA
PmeI         3536  GTTTAAAC             GTTT^AAAC
Psp1406I     1470  AACGTT               AA^CGTT
RcaI          633  TCATGA               T^CATGA
SapI          377  GAAGAGC              GCTCTTC(1/4)
ScaI         2077  AGTACT               AGT^ACT
SfuI         3049  TTCGAA               TT^CGAA
SmaI          180  CCCGGG               CCC^GGG
SspBI        2016  TGTACA               T^GTACA
XmaCI         180  CCCGGG               C^CCGGG
XmaI          180  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI         1348  GTATAC               GT^[AC][GT]AC
             2580  GTATAC              
AciI         2110  CCGC                 CCGC(-3/-1)
             3568  GCGG                
Alw44I        228  GTGCAC               G^TGCAC
             3413  GTGCAC              
AlwNI        2187  CAGTTACTG            CAG[ACGT][ACGT][ACGT]^CTG
             2826  CAGTAACTG           
AocI         3383  CCTTAGG              CC^T[ACGT]AGG
             3623  CCTGAGG             
ApaLI         228  GTGCAC               G^TGCAC
             3413  GTGCAC              
AvaI          117  CTCGGG               C^[CT]CG[GA]G
              180  CCCGGG              
BbvCI         773  CCTCAGC              CCTCAGC(-5/-2)
             3740  GCTGAGG             
BsaI         2554  GAGACC               GGTCTC(1/5)
             3640  GAGACC              
BsaWI         232  ACCGGA               [AT]^CCGG[AT]
              741  ACCGGA              
BseRI          38  GAGGAG               GAGGAG(10/8)
              497  GAGGAG              
BsmI          430  GCATTC               GAATGC(1/-1)
             1926  GCATTC              
BsoBI         117  CTCGGG               C^[CT]CG[GA]G
              180  CCCGGG              
BsrFI        1039  GCCGGT               [GA]^CCGG[CT]
             3696  GCCGGT              
Bst1107I     1348  GTATAC               GTA^TAC
             2580  GTATAC              
BstXI         336  CCAGCAGCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2317  CCAAAGAGGTGG        
BstZ17I      1348  GTATAC               GTA^TAC
             2580  GTATAC              
Bsu36I       3383  CCTTAGG              CC^T[ACGT]AGG
             3623  CCTGAGG             
Cfr10I       1039  GCCGGT               [GA]^CCGG[CT]
             3696  GCCGGT              
DraIII       1672  CACAATGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2697  CACAAAGTG           
Eco81I       3383  CCTTAGG              CC^T[ACGT]AGG
             3623  CCTGAGG             
EcoRV         857  GATATC               GAT^ATC
             3311  GATATC              
EcoT14I       624  CCAAGG               C^C[AT][AT]GG
             1862  CCTAGG              
MaeII        1471  ACGT                 A^CGT
             2104  ACGT                
MslI         1163  CACGCCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2334  CATCAGAATG          
PstI          407  CTGCAG               CTGCA^G
             1835  CTGCAG              
SacI          637  GAGCTC               GAGCT^C
              785  GAGCTC              
StyI          624  CCAAGG               C^C[AT][AT]GG
             1862  CCTAGG              
XcmI          403  CCAGCTGCAGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2696  CCACAAAGTGCTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   86 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        ApoI        
Asp700I     AspHI       AvaII       BalI        BanI        
BanII       BbvI        BcgI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EaeI        EarI        EcoRI       EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XhoII       
XmnI