################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII AccII AccIII AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI AspI AviII BbeI BbrPI BbsI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsiWI BspDI BspEI BsrDI BssHII BssSI BstUI CelII CfoI ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MamI MluI MroI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmlI PpuMI PshAI PshBI PvuI RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 2879 GGTACC G^GTACC AclI 1470 AACGTT AA^CGTT AflIII 2203 ACATGT A^C[GA][CT]GT Asp718I 2879 GGTACC G^GTACC AvrII 1862 CCTAGG C^CTAGG BamHI 1050 GGATCC G^GATCC BlnI 1862 CCTAGG C^CTAGG BsiEI 3698 CGGTCG CG[GA][CT]^CG BsmBI 1714 GAGACG CGTCTC(1/5) BsmFI 874 GGGAC GGGAC(10/14) Bsp1407I 2016 TGTACA T^GTACA BspHI 633 TCATGA T^CATGA BspLU11I 2203 ACATGT A^CATGT BspMI 1029 ACCTGC ACCTGC(4/8) BsrBI 3566 GAGCGG CCGCTC(-3/-3) BsrGI 2016 TGTACA T^GTACA BstBI 3049 TTCGAA TT^CGAA BstEII 479 GGTGACC G^GT[ACGT]ACC BstPI 479 GGTGACC G^GT[ACGT]ACC EcoNI 2127 CCTTTATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 479 GGTGACC G^GT[ACGT]ACC EcoT22I 2751 ATGCAT ATGCA^T HgaI 350 GACGC GACGC(5/10) HindIII 1755 AAGCTT A^AGCTT KpnI 2879 GGTACC GGTAC^C MfeI 3452 CAATTG C^AATTG MunI 3452 CAATTG C^AATTG NdeI 3430 CATATG CA^TATG NsiI 2751 ATGCAT ATGCA^T NspV 3049 TTCGAA TT^CGAA PmeI 3536 GTTTAAAC GTTT^AAAC Psp1406I 1470 AACGTT AA^CGTT RcaI 633 TCATGA T^CATGA SapI 377 GAAGAGC GCTCTTC(1/4) ScaI 2077 AGTACT AGT^ACT SfuI 3049 TTCGAA TT^CGAA SmaI 180 CCCGGG CCC^GGG SspBI 2016 TGTACA T^GTACA XmaCI 180 CCCGGG C^CCGGG XmaI 180 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 1348 GTATAC GT^[AC][GT]AC 2580 GTATAC AciI 2110 CCGC CCGC(-3/-1) 3568 GCGG Alw44I 228 GTGCAC G^TGCAC 3413 GTGCAC AlwNI 2187 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG 2826 CAGTAACTG AocI 3383 CCTTAGG CC^T[ACGT]AGG 3623 CCTGAGG ApaLI 228 GTGCAC G^TGCAC 3413 GTGCAC AvaI 117 CTCGGG C^[CT]CG[GA]G 180 CCCGGG BbvCI 773 CCTCAGC CCTCAGC(-5/-2) 3740 GCTGAGG BsaI 2554 GAGACC GGTCTC(1/5) 3640 GAGACC BsaWI 232 ACCGGA [AT]^CCGG[AT] 741 ACCGGA BseRI 38 GAGGAG GAGGAG(10/8) 497 GAGGAG BsmI 430 GCATTC GAATGC(1/-1) 1926 GCATTC BsoBI 117 CTCGGG C^[CT]CG[GA]G 180 CCCGGG BsrFI 1039 GCCGGT [GA]^CCGG[CT] 3696 GCCGGT Bst1107I 1348 GTATAC GTA^TAC 2580 GTATAC BstXI 336 CCAGCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2317 CCAAAGAGGTGG BstZ17I 1348 GTATAC GTA^TAC 2580 GTATAC Bsu36I 3383 CCTTAGG CC^T[ACGT]AGG 3623 CCTGAGG Cfr10I 1039 GCCGGT [GA]^CCGG[CT] 3696 GCCGGT DraIII 1672 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG 2697 CACAAAGTG Eco81I 3383 CCTTAGG CC^T[ACGT]AGG 3623 CCTGAGG EcoRV 857 GATATC GAT^ATC 3311 GATATC EcoT14I 624 CCAAGG C^C[AT][AT]GG 1862 CCTAGG MaeII 1471 ACGT A^CGT 2104 ACGT MslI 1163 CACGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2334 CATCAGAATG PstI 407 CTGCAG CTGCA^G 1835 CTGCAG SacI 637 GAGCTC GAGCT^C 785 GAGCTC StyI 624 CCAAGG C^C[AT][AT]GG 1862 CCTAGG XcmI 403 CCAGCTGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2696 CCACAAAGTGCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BalI BanI BanII BbvI BcgI BcnI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI EaeI EarI EcoRI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI