################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI AspEI AvrII BbrPI BbsI BciVI BclI BfrI BlnI BpuAI BsaAI BseAI BsiWI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eam1105I EcoRI EcoRV EcoT22I FbaI HindIII HpaI KspI MfeI MluI MroI MseI MunI NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI Tru9I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AccI 614 GTCGAC GT^[AC][GT]AC AocI 2339 CCTGAGG CC^T[ACGT]AGG Asp700I 1912 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 321 GCAAGTACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 841 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1331 GAGACG CGTCTC(1/5) BsmI 274 GAATGC GAATGC(1/-1) BstEII 1885 GGTCACC G^GT[ACGT]ACC BstPI 1885 GGTCACC G^GT[ACGT]ACC Bsu36I 2339 CCTGAGG CC^T[ACGT]AGG DraIII 3362 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 2339 CCTGAGG CC^T[ACGT]AGG EcoO65I 1885 GGTCACC G^GT[ACGT]ACC FseI 2670 GGCCGGCC GGCCGG^CC MamI 841 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 3264 CATATG CA^TATG NotI 4258 GCGGCCGC GC^GGCCGC SacI 3681 GAGCTC GAGCT^C SalI 614 GTCGAC G^TCGAC SapI 709 GAAGAGC GCTCTTC(1/4) SbfI 2888 CCTGCAGG CCTGCA^GG Sse8387I 2888 CCTGCAGG CCTGCA^GG TfiI 1310 GATTC G^A[AT]TC XmnI 1912 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### Aor51HI 481 AGCGCT AGC^GCT 4146 AGCGCT BsaWI 2599 ACCGGA [AT]^CCGG[AT] 3400 TCCGGT BsiEI 3572 CGGCCG CG[GA][CT]^CG 4259 CGGCCG Bsp1407I 1227 TGTACA T^GTACA 1429 TGTACA BsrDI 3751 CATTGC GCAATG(2/0) 2549 GCAATG BsrGI 1227 TGTACA T^GTACA 1429 TGTACA EagI 3572 CGGCCG C^GGCCG 4259 CGGCCG EclXI 3572 CGGCCG C^GGCCG 4259 CGGCCG Eco47III 481 AGCGCT AGC^GCT 4146 AGCGCT Eco52I 3572 CGGCCG C^GGCCG 4259 CGGCCG HincII 614 GTCGAC GT[CT]^[GA]AC 647 GTCAAC HindII 614 GTCGAC GT[CT]^[GA]AC 647 GTCAAC NspI 132 GCATGC [GA]CATG^[CT] 764 GCATGC PleI 3084 GACTC GAGTC(4/5) 352 GAGTC SexAI 1716 ACCAGGT A^CC[AT]GGT 3363 ACCTGGT SphI 132 GCATGC GCATG^C 764 GCATGC SspBI 1227 TGTACA T^GTACA 1429 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 137 enzymes) # ######################################### Acc65I AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI Asp718I AspHI AspI AvaI AvaII AviII BalI BamHI BanI BanII BbeI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflFI PflMI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoII