##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        ApoI        AscI        
AseI        AsnI        AspEI       AvrII       BbrPI       
BbsI        BciVI       BclI        BfrI        BlnI        
BpuAI       BsaAI       BseAI       BsiWI       BspDI       
BspEI       BspHI       BspLU11I    BssHII      Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
DrdI        Eam1105I    EcoRI       EcoRV       EcoT22I     
FbaI        HindIII     HpaI        KspI        MfeI        
MluI        MroI        MseI        MunI        NheI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       ScaI        SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SspI        SwaI        
Tru9I       XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AccI          614  GTCGAC               GT^[AC][GT]AC
AocI         2339  CCTGAGG              CC^T[ACGT]AGG
Asp700I      1912  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI          321  GCAAGTACCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI         841  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI        1331  GAGACG               CGTCTC(1/5)
BsmI          274  GAATGC               GAATGC(1/-1)
BstEII       1885  GGTCACC              G^GT[ACGT]ACC
BstPI        1885  GGTCACC              G^GT[ACGT]ACC
Bsu36I       2339  CCTGAGG              CC^T[ACGT]AGG
DraIII       3362  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I       2339  CCTGAGG              CC^T[ACGT]AGG
EcoO65I      1885  GGTCACC              G^GT[ACGT]ACC
FseI         2670  GGCCGGCC             GGCCGG^CC
MamI          841  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI         3264  CATATG               CA^TATG
NotI         4258  GCGGCCGC             GC^GGCCGC
SacI         3681  GAGCTC               GAGCT^C
SalI          614  GTCGAC               G^TCGAC
SapI          709  GAAGAGC              GCTCTTC(1/4)
SbfI         2888  CCTGCAGG             CCTGCA^GG
Sse8387I     2888  CCTGCAGG             CCTGCA^GG
TfiI         1310  GATTC                G^A[AT]TC
XmnI         1912  GAAGTTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

Aor51HI       481  AGCGCT               AGC^GCT
             4146  AGCGCT              
BsaWI        2599  ACCGGA               [AT]^CCGG[AT]
             3400  TCCGGT              
BsiEI        3572  CGGCCG               CG[GA][CT]^CG
             4259  CGGCCG              
Bsp1407I     1227  TGTACA               T^GTACA
             1429  TGTACA              
BsrDI        3751  CATTGC               GCAATG(2/0)
             2549  GCAATG              
BsrGI        1227  TGTACA               T^GTACA
             1429  TGTACA              
EagI         3572  CGGCCG               C^GGCCG
             4259  CGGCCG              
EclXI        3572  CGGCCG               C^GGCCG
             4259  CGGCCG              
Eco47III      481  AGCGCT               AGC^GCT
             4146  AGCGCT              
Eco52I       3572  CGGCCG               C^GGCCG
             4259  CGGCCG              
HincII        614  GTCGAC               GT[CT]^[GA]AC
              647  GTCAAC              
HindII        614  GTCGAC               GT[CT]^[GA]AC
              647  GTCAAC              
NspI          132  GCATGC               [GA]CATG^[CT]
              764  GCATGC              
PleI         3084  GACTC                GAGTC(4/5)
              352  GAGTC               
SexAI        1716  ACCAGGT              A^CC[AT]GGT
             3363  ACCTGGT             
SphI          132  GCATGC               GCATG^C
              764  GCATGC              
SspBI        1227  TGTACA               T^GTACA
             1429  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

Acc65I      AccII       AciI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       Asp718I     AspHI       AspI        AvaI        
AvaII       AviII       BalI        BamHI       BanI        
BanII       BbeI        BbvCI       BbvI        BcnI        
BfaI        BglI        BglII       BlpI        BmyI        
BpmI        Bpu1102I    BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrBI       BsrFI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        KpnI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       PflFI       PflMI       PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmlI        
StuI        StyI        TaqI        TseI        Tsp45I      
Tsp509I     TspRI       Tth111I     TthHB8I     Van91I      
XcmI        XhoII