##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
Aor51HI AscI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BcgI
BciVI BclI BglII BlnI BlpI
BpmI Bpu1102I BsiEI BsiWI BspDI
BssHII BssSI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
FbaI FseI FspI KasI KpnI
KspI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmeI
PshAI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 122 GTCTAC GT^[AC][GT]AC
AccII 286 CGCG CG^CG
AcyI 176 GACGCC G[GA]^CG[CT]C
AlwNI 1019 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2434 CCTTAGG CC^T[ACGT]AGG
ApaI 915 GGGCCC GGGCC^C
AvaI 1316 CTCGAG C^[CT]CG[GA]G
BalI 108 TGGCCA TGG^CCA
BbrPI 2469 CACGTG CAC^GTG
BbvCI 470 GCTGAGG CCTCAGC(-5/-2)
BglI 95 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2469 CACGTG [CT]AC^GT[GA]
BsaHI 176 GACGCC G[GA]^CG[CT]C
BsgI 854 GTGCAG GTGCAG(16/14)
BsmBI 1119 GAGACG CGTCTC(1/5)
BsmI 481 GAATGC GAATGC(1/-1)
BsoBI 1316 CTCGAG C^[CT]CG[GA]G
BspHI 544 TCATGA T^CATGA
BspLU11I 1488 ACATGT A^CATGT
BsrBI 1033 CCGCTC CCGCTC(-3/-3)
BsrFI 1157 GCCGGT [GA]^CCGG[CT]
BstUI 286 CGCG CG^CG
Bsu36I 2434 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1157 GCCGGT [GA]^CCGG[CT]
DraIII 2704 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 108 TGGCCA [CT]^GGCC[GA]
Eco81I 2434 CCTTAGG CC^T[ACGT]AGG
EcoRV 1978 GATATC GAT^ATC
HaeII 103 GGCGCT [GA]GCGC^[CT]
Hin1I 176 GACGCC G[GA]^CG[CT]C
MluNI 108 TGGCCA TGG^CCA
MscI 108 TGGCCA TGG^CCA
MslI 2277 CACTTATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 286 CGCG CG^CG
PaeR7I 1316 CTCGAG C^TCGAG
PleI 1689 GACTC GAGTC(4/5)
PmaCI 2469 CACGTG CAC^GTG
PmlI 2469 CACGTG CAC^GTG
PpuMI 1600 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 544 TCATGA T^CATGA
SapI 371 GAAGAGC GCTCTTC(1/4)
SexAI 2705 ACCTGGT A^CC[AT]GGT
SphI 1968 GCATGC GCATG^C
XbaI 1869 TCTAGA T^CTAGA
XhoI 1316 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 1248 TCCGGA T^CCGGA
3599 TCCGGA
AflII 1476 CTTAAG C^TTAAG
2369 CTTAAG
Alw44I 192 GTGCAC G^TGCAC
1741 GTGCAC
ApaLI 192 GTGCAC G^TGCAC
1741 GTGCAC
Asp700I 1631 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2968 GAACCATTTC
AspHI 192 GTGCAC G[AT]GC[AT]^C
1741 GTGCAC
BanI 2702 GGCACC G^G[CT][GA]CC
2948 GGCACC
BanII 915 GGGCCC G[GA]GC[CT]^C
1319 GAGCCC
BbsI 292 GTCTTC GAAGAC(2/6)
2787 GTCTTC
BfrI 1476 CTTAAG C^TTAAG
2369 CTTAAG
BpuAI 292 GTCTTC GAAGAC(2/6)
2787 GTCTTC
BsaBI 1890 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3411 GATAACTATC
BsaI 5 GGTCTC GGTCTC(1/5)
3240 GGTCTC
BsaWI 1248 TCCGGA [AT]^CCGG[AT]
3599 TCCGGA
BseAI 1248 TCCGGA T^CCGGA
3599 TCCGGA
BsiHKAI 192 GTGCAC G[AT]GC[AT]^C
1741 GTGCAC
BspEI 1248 TCCGGA T^CCGGA
3599 TCCGGA
BstAPI 615 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2629 GCACACACTGC
BstEII 310 GGTGACC G^GT[ACGT]ACC
1168 GGTGACC
BstPI 310 GGTGACC G^GT[ACGT]ACC
1168 GGTGACC
BstXI 37 CCAGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1140 CCAAGCGTTTGG
DsaI 403 CCACGG C^C[GA][CT]GG
2952 CCACGG
EcoNI 1608 CCTTCATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2236 CCTTTAGAAGG
EcoO65I 310 GGTGACC G^GT[ACGT]ACC
1168 GGTGACC
EcoT22I 2545 ATGCAT ATGCA^T
3944 ATGCAT
HgaI 176 GACGC GACGC(5/10)
390 GCGTC
HpaI 869 GTTAAC GTT^AAC
1709 GTTAAC
MamI 1890 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3411 GATAACTATC
MfeI 2831 CAATTG C^AATTG
3537 CAATTG
MroI 1248 TCCGGA T^CCGGA
3599 TCCGGA
MspA1I 42 CCGCTG C[AC]G^C[GT]G
131 CCGCTG
MunI 2831 CAATTG C^AATTG
3537 CAATTG
NsiI 2545 ATGCAT ATGCA^T
3944 ATGCAT
PflMI 217 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3463 CCAATATTTGG
PstI 1067 CTGCAG CTGCA^G
3733 CTGCAG
ScaI 1756 AGTACT AGT^ACT
3756 AGTACT
Van91I 217 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3463 CCAATATTTGG
XmnI 1631 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2968 GAACCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AvaII
BbvI BcnI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PshBI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII