##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AviII BamHI BbrPI BbsI
BpuAI BsaBI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
HgaI HindIII HpaI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SexAI SfiI SfuI SgrAI
SnaBI SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AatII 2377 GACGTC GACGT^C
Acc65I 2313 GGTACC G^GTACC
AccI 1900 GTCTAC GT^[AC][GT]AC
AflII 3884 CTTAAG C^TTAAG
Asp718I 2313 GGTACC G^GTACC
BcgI 1682 CGACCCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 915 GTATCC GTATCC(6/5)
BfrI 3884 CTTAAG C^TTAAG
BglII 1128 AGATCT A^GATCT
BlpI 510 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 510 GCTCAGC GC^T[ACGT]AGC
BsaAI 1903 TACGTG [CT]AC^GT[GA]
BspHI 1289 TCATGA T^CATGA
BsrFI 2079 ACCGGC [GA]^CCGG[CT]
CelII 510 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2079 ACCGGC [GA]^CCGG[CT]
DraI 3804 TTTAAA TTT^AAA
EcoNI 2286 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2865 ATGCAT ATGCA^T
HincII 931 GTCAAC GT[CT]^[GA]AC
HindII 931 GTCAAC GT[CT]^[GA]AC
KpnI 2313 GGTACC GGTAC^C
NsiI 2865 ATGCAT ATGCA^T
RcaI 1289 TCATGA T^CATGA
SacI 469 GAGCTC GAGCT^C
SbfI 2121 CCTGCAGG CCTGCA^GG
ScaI 1964 AGTACT AGT^ACT
SpeI 678 ACTAGT A^CTAGT
Sse8387I 2121 CCTGCAGG CCTGCA^GG
XcmI 3429 CCAGAGCACCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 2622 ACATGT A^C[GA][CT]GT
3172 ACATGT
AocI 73 CCTGAGG CC^T[ACGT]AGG
2286 CCTGAGG
AspI 901 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4251 GACTCCGTC
AvrII 21 CCTAGG C^CTAGG
2940 CCTAGG
BbvCI 3271 CCTCAGC CCTCAGC(-5/-2)
2296 GCTGAGG
BclI 158 TGATCA T^GATCA
3023 TGATCA
BlnI 21 CCTAGG C^CTAGG
2940 CCTAGG
BsaI 2076 GAGACC GGTCTC(1/5)
788 GGTCTC
BsmBI 560 CGTCTC CGTCTC(1/5)
4256 CGTCTC
BsmI 1710 GAATGC GAATGC(1/-1)
3814 GCATTC
BspLU11I 2622 ACATGT A^CATGT
3172 ACATGT
BsrBI 3329 CCGCTC CCGCTC(-3/-3)
2254 GAGCGG
BstYI 1128 AGATCT [GA]^GATC[CT]
1447 AGATCC
Bsu36I 73 CCTGAGG CC^T[ACGT]AGG
2286 CCTGAGG
Eco81I 73 CCTGAGG CC^T[ACGT]AGG
2286 CCTGAGG
FbaI 158 TGATCA T^GATCA
3023 TGATCA
MflI 1128 AGATCT [GA]^GATC[CT]
1447 AGATCC
NcoI 1337 CCATGG C^CATGG
3438 CCATGG
PflFI 901 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4251 GACTCCGTC
PflMI 1136 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1686 CCAGCTGCTGG
PpuMI 2115 AGGACCC [GA]G^G[AT]CC[CT]
2614 AGGACCT
SmaI 740 CCCGGG CCC^GGG
2675 CCCGGG
Tth111I 901 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4251 GACTCCGTC
Van91I 1136 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1686 CCAGCTGCTGG
XhoII 1128 AGATCT [GA]^GATC[CT]
1447 AGATCC
XmaCI 740 CCCGGG C^CCGGG
2675 CCCGGG
XmaI 740 CCCGGG C^CCGGG
2675 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI