##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaII       AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BlnI        BsaAI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BspEI       
BspLU11I    BspMI       BsrBI       BsrFI       BssHII      
BssSI       BstBI       BstUI       BstXI       Bsu36I      
Cfr10I      CpoI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoT14I     FauI        FbaI        FseI        
FspI        HaeII       HincII      HindII      HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NotI        NruI        NspI        NspV        PacI        
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    StyI        SwaI        Tth111I     Van91I      
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   58 enzymes) #
###################################

AccI         1919  GTATAC               GT^[AC][GT]AC
AciI          140  CCGC                 CCGC(-3/-1)
AcyI           19  GGCGTC               G[GA]^CG[CT]C
ApaI          257  GGGCCC               GGGCC^C
Asp700I      1422  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI            6  CTCGAG               C^[CT]CG[GA]G
BanI           11  GGTGCC               G^G[CT][GA]CC
BcnI          317  CCGGG                CC^[GC]GG
BglII        1424  AGATCT               A^GATCT
BlpI         1583  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     1583  GCTTAGC              GC^T[ACGT]AGC
BsaHI          19  GGCGTC               G[GA]^CG[CT]C
BsaJI          15  CCACGG               C^C[ACGT][ACGT]GG
BsiYI         140  CCGCACTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          140  CCGCACTTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI        1640  GGGAC                GGGAC(10/14)
BsmI          905  GCATTC               GAATGC(1/-1)
BsoBI           6  CTCGAG               C^[CT]CG[GA]G
Bsp1407I      931  TGTACA               T^GTACA
BspDI         519  ATCGAT               AT^CGAT
BspHI        1544  TCATGA               T^CATGA
BsrGI         931  TGTACA               T^GTACA
Bst1107I     1919  GTATAC               GTA^TAC
BstEII        863  GGTTACC              G^GT[ACGT]ACC
BstPI         863  GGTTACC              G^GT[ACGT]ACC
BstZ17I      1919  GTATAC               GTA^TAC
CelII        1583  GCTTAGC              GC^T[ACGT]AGC
CfoI         1266  GCGC                 GCG^C
ClaI          519  ATCGAT               AT^CGAT
DraI          590  TTTAAA               TTT^AAA
DsaI           15  CCACGG               C^C[GA][CT]GG
EcoO65I       863  GGTTACC              G^GT[ACGT]ACC
EcoRI        1695  GAATTC               G^AATTC
EcoRV        1056  GATATC               GAT^ATC
EcoT22I      1822  ATGCAT               ATGCA^T
HapII         317  CCGG                 C^CGG
HgaI           20  GCGTC                GACGC(5/10)
HhaI         1266  GCGC                 GCG^C
Hin1I          19  GGCGTC               G[GA]^CG[CT]C
HinP1I       1266  GCGC                 G^CGC
HindIII      1581  AAGCTT               A^AGCTT
HpaII         317  CCGG                 C^CGG
MaeII         407  ACGT                 A^CGT
MspI          317  CCGG                 C^CGG
NciI          317  CCGGG                CC^[GC]GG
NheI          995  GCTAGC               G^CTAGC
NsiI         1822  ATGCAT               ATGCA^T
PaeR7I          6  CTCGAG               C^TCGAG
RcaI         1544  TCATGA               T^CATGA
SapI         1560  GCTCTTC              GCTCTTC(1/4)
SexAI         326  ACCTGGT              A^CC[AT]GGT
SspBI         931  TGTACA               T^GTACA
SspI         2082  AATATT               AAT^ATT
StuI         1085  AGGCCT               AGG^CCT
Tsp45I       1955  GTCAC                ^GT[GC]AC
TspRI        1722  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoI            6  CTCGAG               C^TCGAG
XmnI         1422  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI         1695  GAATTC               [GA]^AATT[CT]
             1741  AAATTT              
AlwI          320  GGATC                GGATC(4/5)
              428  GGATC               
AlwNI        1638  CAGGGACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1935  CAGCTACTG           
ApoI         1695  GAATTC               [GA]^AATT[CT]
             1741  AAATTT              
BanII           3  GGGCTC               G[GA]GC[CT]^C
              257  GGGCCC              
BbsI          335  GAAGAC               GAAGAC(2/6)
             1608  GAAGAC              
BmyI            3  GGGCTC               G[AGT]GC[ACT]^C
              257  GGGCCC              
BpuAI         335  GAAGAC               GAAGAC(2/6)
             1608  GAAGAC              
Bsp1286I        3  GGGCTC               G[AGT]GC[ACT]^C
              257  GGGCCC              
BstAPI       1409  GCAGACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1934  GCAGCTACTGC         
DraII         256  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              257  GGGCCCT             
EaeI          137  TGGCCG               [CT]^GGCC[GA]
              394  TGGCCG              
EarI         1561  CTCTTC               CTCTTC(1/4)
              756  GAAGAG              
EcoO109I      256  GGGGCCC              [GA]G^G[ACGT]CC[CT]
              257  GGGCCCT             
HphI         2033  GGTGA                GGTGA(8/7)
             1788  TCACC               
Ksp632I      1561  CTCTTC               CTCTTC(1/4)
              756  GAAGAG              
MslI         1124  CATAGAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1944  CATCTTCATG          
PstI         1219  CTGCAG               CTGCA^G
             1332  CTGCAG              
SmlI            6  CTCGAG               C^T[CT][GA]AG
              434  CTTGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   53 enzymes) #
#########################################

AfaI        AluI        BbvI        BfaI        BpmI        
BsaBI       BsmAI       BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      Fnu4HI      FokI        
HaeIII      HinfI       ItaI        MaeI        MaeIII      
MamI        MboI        MboII       MflI        MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TthHB8I     XcmI        XhoII