################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI BstBI BstUI BstXI Bsu36I Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoT14I FauI FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StyI SwaI Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 1919 GTATAC GT^[AC][GT]AC AciI 140 CCGC CCGC(-3/-1) AcyI 19 GGCGTC G[GA]^CG[CT]C ApaI 257 GGGCCC GGGCC^C Asp700I 1422 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 6 CTCGAG C^[CT]CG[GA]G BanI 11 GGTGCC G^G[CT][GA]CC BcnI 317 CCGGG CC^[GC]GG BglII 1424 AGATCT A^GATCT BlpI 1583 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1583 GCTTAGC GC^T[ACGT]AGC BsaHI 19 GGCGTC G[GA]^CG[CT]C BsaJI 15 CCACGG C^C[ACGT][ACGT]GG BsiYI 140 CCGCACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 140 CCGCACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 1640 GGGAC GGGAC(10/14) BsmI 905 GCATTC GAATGC(1/-1) BsoBI 6 CTCGAG C^[CT]CG[GA]G Bsp1407I 931 TGTACA T^GTACA BspDI 519 ATCGAT AT^CGAT BspHI 1544 TCATGA T^CATGA BsrGI 931 TGTACA T^GTACA Bst1107I 1919 GTATAC GTA^TAC BstEII 863 GGTTACC G^GT[ACGT]ACC BstPI 863 GGTTACC G^GT[ACGT]ACC BstZ17I 1919 GTATAC GTA^TAC CelII 1583 GCTTAGC GC^T[ACGT]AGC CfoI 1266 GCGC GCG^C ClaI 519 ATCGAT AT^CGAT DraI 590 TTTAAA TTT^AAA DsaI 15 CCACGG C^C[GA][CT]GG EcoO65I 863 GGTTACC G^GT[ACGT]ACC EcoRI 1695 GAATTC G^AATTC EcoRV 1056 GATATC GAT^ATC EcoT22I 1822 ATGCAT ATGCA^T HapII 317 CCGG C^CGG HgaI 20 GCGTC GACGC(5/10) HhaI 1266 GCGC GCG^C Hin1I 19 GGCGTC G[GA]^CG[CT]C HinP1I 1266 GCGC G^CGC HindIII 1581 AAGCTT A^AGCTT HpaII 317 CCGG C^CGG MaeII 407 ACGT A^CGT MspI 317 CCGG C^CGG NciI 317 CCGGG CC^[GC]GG NheI 995 GCTAGC G^CTAGC NsiI 1822 ATGCAT ATGCA^T PaeR7I 6 CTCGAG C^TCGAG RcaI 1544 TCATGA T^CATGA SapI 1560 GCTCTTC GCTCTTC(1/4) SexAI 326 ACCTGGT A^CC[AT]GGT SspBI 931 TGTACA T^GTACA SspI 2082 AATATT AAT^ATT StuI 1085 AGGCCT AGG^CCT Tsp45I 1955 GTCAC ^GT[GC]AC TspRI 1722 CACTG CA[GC]TG[ACGT][ACGT]^ XhoI 6 CTCGAG C^TCGAG XmnI 1422 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 1695 GAATTC [GA]^AATT[CT] 1741 AAATTT AlwI 320 GGATC GGATC(4/5) 428 GGATC AlwNI 1638 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG 1935 CAGCTACTG ApoI 1695 GAATTC [GA]^AATT[CT] 1741 AAATTT BanII 3 GGGCTC G[GA]GC[CT]^C 257 GGGCCC BbsI 335 GAAGAC GAAGAC(2/6) 1608 GAAGAC BmyI 3 GGGCTC G[AGT]GC[ACT]^C 257 GGGCCC BpuAI 335 GAAGAC GAAGAC(2/6) 1608 GAAGAC Bsp1286I 3 GGGCTC G[AGT]GC[ACT]^C 257 GGGCCC BstAPI 1409 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1934 GCAGCTACTGC DraII 256 GGGGCCC [GA]G^G[ACGT]CC[CT] 257 GGGCCCT EaeI 137 TGGCCG [CT]^GGCC[GA] 394 TGGCCG EarI 1561 CTCTTC CTCTTC(1/4) 756 GAAGAG EcoO109I 256 GGGGCCC [GA]G^G[ACGT]CC[CT] 257 GGGCCCT HphI 2033 GGTGA GGTGA(8/7) 1788 TCACC Ksp632I 1561 CTCTTC CTCTTC(1/4) 756 GAAGAG MslI 1124 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1944 CATCTTCATG PstI 1219 CTGCAG CTGCA^G 1332 CTGCAG SmlI 6 CTCGAG C^T[CT][GA]AG 434 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AfaI AluI BbvI BfaI BpmI BsaBI BsmAI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MamI MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TthHB8I XcmI XhoII