##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
Acc65I AccIII AclI AgeI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbrPI
BcgI BclI BglII BlnI BsaAI
BsaBI BseAI BsiWI BsmBI BsmI
BspDI BspEI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII EagI EclXI Eco52I EcoO65I
EcoRV FbaI FspI HpaI KpnI
MamI MfeI MroI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 726 GACGTC GACGT^C
AflII 3234 CTTAAG C^TTAAG
AocI 2492 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1771 AGCGCT AGC^GCT
AspI 219 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1053 GGCGCC GGCGC^C
BfrI 3234 CTTAAG C^TTAAG
BsiEI 464 CGACCG CG[GA][CT]^CG
BspHI 748 TCATGA T^CATGA
BspLU11I 2007 ACATGT A^CATGT
BsrDI 2709 CATTGC GCAATG(2/0)
BssSI 957 CTCGTG CACGAG(-5/-1)
Bsu36I 2492 CCTCAGG CC^T[ACGT]AGG
DraI 3663 TTTAAA TTT^AAA
DrdI 366 GACTTCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1771 AGCGCT AGC^GCT
Eco81I 2492 CCTCAGG CC^T[ACGT]AGG
EcoNI 2481 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 261 GAATTC G^AATTC
EcoT22I 2394 ATGCAT ATGCA^T
FseI 618 GGCCGGCC GGCCGG^CC
HindIII 2371 AAGCTT A^AGCTT
KasI 1053 GGCGCC G^GCGCC
KspI 1339 CCGCGG CCGC^GG
MluI 908 ACGCGT A^CGCGT
NarI 1053 GGCGCC GG^CGCC
NsiI 2394 ATGCAT ATGCA^T
PflFI 219 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1893 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 748 TCATGA T^CATGA
SacII 1339 CCGCGG CCGC^GG
SapI 989 GCTCTTC GCTCTTC(1/4)
SfiI 1496 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1053 GGCGCC GGC^GCC
SspI 3688 AATATT AAT^ATT
TaqI 841 TCGA T^CGA
Tth111I 219 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 841 TCGA T^CGA
XcmI 188 CCACATCGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 579 GTCTAC GT^[AC][GT]AC
3514 GTAGAC
AflIII 908 ACGCGT A^C[GA][CT]GT
2007 ACATGT
AhdI 342 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
564 GACTACCAGTC
AspEI 342 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
564 GACTACCAGTC
BbsI 1900 GTCTTC GAAGAC(2/6)
3324 GTCTTC
BbvCI 2972 CCTCAGC CCTCAGC(-5/-2)
2556 GCTGAGG
BciVI 806 GGATAC GTATCC(6/5)
2818 GGATAC
BlpI 1172 GCTGAGC GC^T[ACGT]AGC
1280 GCTTAGC
BpmI 951 CTGGAG CTGGAG(16/14)
2101 CTGGAG
Bpu1102I 1172 GCTGAGC GC^T[ACGT]AGC
1280 GCTTAGC
BpuAI 1900 GTCTTC GAAGAC(2/6)
3324 GTCTTC
BsaWI 1475 ACCGGA [AT]^CCGG[AT]
3067 ACCGGA
Bsp1407I 448 TGTACA T^GTACA
3653 TGTACA
BsrBI 57 CCGCTC CCGCTC(-3/-3)
2745 GAGCGG
BsrGI 448 TGTACA T^GTACA
3653 TGTACA
BstYI 241 AGATCC [GA]^GATC[CT]
1845 GGATCT
CelII 1172 GCTGAGC GC^T[ACGT]AGC
1280 GCTTAGC
Eam1105I 342 GACGGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
564 GACTACCAGTC
HincII 45 GTCAAC GT[CT]^[GA]AC
291 GTCAAC
HindII 45 GTCAAC GT[CT]^[GA]AC
291 GTCAAC
MflI 241 AGATCC [GA]^GATC[CT]
1845 GGATCT
NaeI 619 GCCGGC GCC^GGC
1069 GCCGGC
NcoI 679 CCATGG C^CATGG
3412 CCATGG
NgoMIV 619 GCCGGC G^CCGGC
1069 GCCGGC
PflMI 521 CCAATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1024 CCACGCGCTGG
PpuMI 781 GGGACCC [GA]G^G[AT]CC[CT]
3557 GGGACCC
PstI 328 CTGCAG CTGCA^G
1380 CTGCAG
SacI 954 GAGCTC GAGCT^C
987 GAGCTC
SspBI 448 TGTACA T^GTACA
3653 TGTACA
Van91I 521 CCAATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1024 CCACGCGCTGG
XhoII 241 AGATCC [GA]^GATC[CT]
1845 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BmyI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XmaCI XmaI