##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

Acc65I      AccIII      AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AvrII       
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BglII       BlnI        BseAI       BsmBI       BspDI       
BspEI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        DrdI        Eco47III    
Eco81I      EcoO65I     EcoRV       FseI        HindIII     
KasI        KpnI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PmaCI       
PmeI        PmlI        PshAI       PshBI       PvuI        
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        SwaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        1068  GACGTC               GACGT^C
AcyI         1068  GACGTC               G[GA]^CG[CT]C
AflII        4111  CTTAAG               C^TTAAG
AflIII         66  ACATGT               A^C[GA][CT]GT
AgeI         2992  ACCGGT               A^CCGGT
AhdI         3515  GACGCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       3211  GTGCAC               G^TGCAC
ApaLI        3211  GTGCAC               G^TGCAC
Asp700I      1324  GAACGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3515  GACGCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3946  TGCGCA               TGC^GCA
BfrI         4111  CTTAAG               C^TTAAG
BglI         2815  GCCCAGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        2785  TACGTG               [CT]AC^GT[GA]
BsaHI        1068  GACGTC               G[GA]^CG[CT]C
BsaI         1835  GAGACC               GGTCTC(1/5)
BsiEI         182  CGGCCG               CG[GA][CT]^CG
BsiWI        3304  CGTACG               C^GTACG
Bsp1407I     3385  TGTACA               T^GTACA
BspHI         437  TCATGA               T^CATGA
BspLU11I       66  ACATGT               A^CATGT
BsrGI        3385  TGTACA               T^GTACA
BssHII       1084  GCGCGC               G^CGCGC
BssSI        3075  CACGAG               CACGAG(-5/-1)
CpoI          871  CGGACCG              CG^G[AT]CCG
DraIII       1552  CACACTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          182  CGGCCG               C^GGCCG
Eam1105I     3515  GACGCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         182  CGGCCG               C^GGCCG
Eco52I        182  CGGCCG               C^GGCCG
EcoNI        3635  CCTACAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I       638  ATGCAT               ATGCA^T
FspI         3946  TGCGCA               TGC^GCA
HaeII        2823  GGCGCT               [GA]GCGC^[CT]
Hin1I        1068  GACGTC               G[GA]^CG[CT]C
HpaI         4068  GTTAAC               GTT^AAC
NdeI          746  CATATG               CA^TATG
NsiI          638  ATGCAT               ATGCA^T
PinAI        2992  ACCGGT               A^CCGGT
RcaI          437  TCATGA               T^CATGA
RsrII         871  CGGACCG              CG^G[AT]CCG
SacI         2368  GAGCTC               GAGCT^C
SapI         4150  GCTCTTC              GCTCTTC(1/4)
SmaI         2970  CCCGGG               CCC^GGG
SphI         1715  GCATGC               GCATG^C
SspBI        3385  TGTACA               T^GTACA
XbaI          544  TCTAGA               T^CTAGA
XmaCI        2970  CCCGGG               C^CCGGG
XmaI         2970  CCCGGG               C^CCGGG
XmnI         1324  GAACGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AccI          559  GTAGAC               GT^[AC][GT]AC
             2308  GTCTAC              
AclI          766  AACGTT               AA^CGTT
             1188  AACGTT              
AspHI        2368  GAGCTC               G[AT]GC[AT]^C
             3211  GTGCAC              
AspI          829  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3285  GACAAGGTC           
BanI         1867  GGTGCC               G^G[CT][GA]CC
             3438  GGTGCC              
BclI          434  TGATCA               T^GATCA
             2322  TGATCA              
BsaBI         429  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1155  GATTCGTATC          
BsgI         2658  CTGCAC               GTGCAG(16/14)
             2350  GTGCAG              
BsiHKAI      2368  GAGCTC               G[AT]GC[AT]^C
             3211  GTGCAC              
BsmI         2105  GAATGC               GAATGC(1/-1)
             2031  GCATTC              
BspMI        2705  ACCTGC               ACCTGC(4/8)
             3835  ACCTGC              
BsrBI        1435  CCGCTC               CCGCTC(-3/-3)
             3443  CCGCTC              
BsrFI        1787  GCCGGT               [GA]^CCGG[CT]
             2992  ACCGGT              
BstAPI        772  GCACTGTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1225  GCAGTCTGTGC         
BstYI         121  AGATCC               [GA]^GATC[CT]
             1039  GGATCT              
Cfr10I       1787  GCCGGT               [GA]^CCGG[CT]
             2992  ACCGGT              
DraI         3858  TTTAAA               TTT^AAA
             3874  TTTAAA              
DraII        2964  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             3521  GGGTCCT             
EcoO109I     2964  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             3521  GGGTCCT             
EcoRI         721  GAATTC               G^AATTC
             3895  GAATTC              
FbaI          434  TGATCA               T^GATCA
             2322  TGATCA              
KspI          108  CCGCGG               CCGC^GG
             2613  CCGCGG              
MamI          429  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1155  GATTCGTATC          
MflI          121  AGATCC               [GA]^GATC[CT]
             1039  GGATCT              
PaeR7I       3249  CTCGAG               C^TCGAG
             3472  CTCGAG              
PflFI         829  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3285  GACAAGGTC           
PflMI        1840  CCATATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4195  CCATTAACTGG         
PpuMI        2964  GGGTCCC              [GA]G^G[AT]CC[CT]
             3521  GGGTCCT             
Psp1406I      766  AACGTT               AA^CGTT
             1188  AACGTT              
SacII         108  CCGCGG               CCGC^GG
             2613  CCGCGG              
StuI         2954  AGGCCT               AGG^CCT
             3495  AGGCCT              
Tth111I       829  GACGCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3285  GACAAGGTC           
Van91I       1840  CCATATTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4195  CCATTAACTGG         
XcmI         1511  CCAAAATTGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1840  CCATATTGTGGATGG     
XhoI         3249  CTCGAG               C^TCGAG
             3472  CTCGAG              
XhoII         121  AGATCC               [GA]^GATC[CT]
             1039  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  104 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaI        AvaII       
BalI        BanII       BbsI        BbvI        BciVI       
BcnI        BfaI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BsaWI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DsaI        
EaeI        EarI        EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeIII      HapII       HgaI        
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I