##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AspI AviII AvrII BanI BbeI
BlnI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BstAPI
BstBI BstEII BstPI ClaI CpoI
DrdI Eco47III EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII KasI
KpnI KspI MluI MroI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI Tth111I
Van91I XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcyI 2404 GACGCC G[GA]^CG[CT]C
AhdI 2231 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1386 GTGCAC G^TGCAC
ApaI 167 GGGCCC GGGCC^C
ApaLI 1386 GTGCAC G^TGCAC
Asp700I 774 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2231 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2309 CCCGGG C^[CT]CG[GA]G
BamHI 482 GGATCC G^GATCC
BbrPI 303 CACGTG CAC^GTG
BbvCI 3638 GCTGAGG CCTCAGC(-5/-2)
BcgI 4217 CGACTGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1430 TGATCA T^GATCA
BglI 1100 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 915 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 915 GCTGAGC GC^T[ACGT]AGC
BsaAI 303 CACGTG [CT]AC^GT[GA]
BsaBI 4081 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2404 GACGCC G[GA]^CG[CT]C
BsiEI 63 CGGCCG CG[GA][CT]^CG
BsoBI 2309 CCCGGG C^[CT]CG[GA]G
BspLU11I 776 ACATGT A^CATGT
BsrBI 3139 GAGCGG CCGCTC(-3/-3)
BssHII 58 GCGCGC G^CGCGC
BssSI 796 CACGAG CACGAG(-5/-1)
Bst1107I 612 GTATAC GTA^TAC
BstZ17I 612 GTATAC GTA^TAC
CelII 915 GCTGAGC GC^T[ACGT]AGC
DraIII 761 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2737 CCATGG C^C[GA][CT]GG
EagI 63 CGGCCG C^GGCCG
Eam1105I 2231 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 63 CGGCCG C^GGCCG
Eco52I 63 CGGCCG C^GGCCG
FbaI 1430 TGATCA T^GATCA
Hin1I 2404 GACGCC G[GA]^CG[CT]C
HindIII 2557 AAGCTT A^AGCTT
MamI 4081 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1081 CAATTG C^AATTG
MunI 1081 CAATTG C^AATTG
NcoI 2737 CCATGG C^CATGG
NspI 776 ACATGT [GA]CATG^[CT]
PmaCI 303 CACGTG CAC^GTG
PmlI 303 CACGTG CAC^GTG
PshAI 1452 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1031 GTCGAC G^TCGAC
SapI 3114 GCTCTTC GCTCTTC(1/4)
ScaI 3388 AGTACT AGT^ACT
SmaI 2309 CCCGGG CCC^GGG
XcmI 1075 CCAGTTCAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2309 CCCGGG C^CCGGG
XmaI 2309 CCCGGG C^CCGGG
XmnI 774 GAACATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 612 GTATAC GT^[AC][GT]AC
1031 GTCGAC
AflII 2952 CTTAAG C^TTAAG
4267 CTTAAG
AflIII 776 ACATGT A^C[GA][CT]GT
4322 ACACGT
AocI 3379 CCTGAGG CC^T[ACGT]AGG
3623 CCTAAGG
BciVI 600 GGATAC GTATCC(6/5)
505 GTATCC
BfrI 2952 CTTAAG C^TTAAG
4267 CTTAAG
BglII 1394 AGATCT A^GATCT
2598 AGATCT
BsaI 1781 GAGACC GGTCTC(1/5)
3975 GAGACC
BspMI 3603 ACCTGC ACCTGC(4/8)
726 GCAGGT
BsrDI 866 GCAATG GCAATG(2/0)
3271 GCAATG
Bsu36I 3379 CCTGAGG CC^T[ACGT]AGG
3623 CCTAAGG
DraI 2786 TTTAAA TTT^AAA
2928 TTTAAA
Eco81I 3379 CCTGAGG CC^T[ACGT]AGG
3623 CCTAAGG
EcoNI 665 CCTGATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2249 CCTCTAGAAGG
HpaI 1836 GTTAAC GTT^AAC
1988 GTTAAC
NdeI 2162 CATATG CA^TATG
3820 CATATG
PpuMI 700 AGGTCCT [GA]G^G[AT]CC[CT]
3590 GGGACCT
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BsrFI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII