##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspI AvaI AviII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BsaBI BsaWI BseAI BsiEI BsmI
BsoBI BspDI BspEI BspMI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRV FauI FbaI FseI
FspI HaeII HhaI HinP1I HincII
HindII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SwaI Tth111I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 1320 GACGTC GACGT^C
AcyI 1320 GACGTC G[GA]^CG[CT]C
AhdI 2444 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 452 GGGCCC GGGCC^C
AseI 3752 ATTAAT AT^TAAT
AsnI 3752 ATTAAT AT^TAAT
AspEI 2444 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2776 CCTAGG C^CTAGG
BanI 967 GGCACC G^G[CT][GA]CC
BanII 452 GGGCCC G[GA]GC[CT]^C
BglII 3243 AGATCT A^GATCT
BlnI 2776 CCTAGG C^CTAGG
BlpI 1610 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1610 GCTCAGC GC^T[ACGT]AGC
BsaAI 1930 TACGTA [CT]AC^GT[GA]
BsaHI 1320 GACGTC G[GA]^CG[CT]C
BsaI 202 GGTCTC GGTCTC(1/5)
BsgI 820 GTGCAG GTGCAG(16/14)
BsiWI 3262 CGTACG C^GTACG
BsmBI 3258 GAGACG CGTCTC(1/5)
Bsp1407I 2096 TGTACA T^GTACA
BspHI 2722 TCATGA T^CATGA
BsrGI 2096 TGTACA T^GTACA
BstEII 551 GGTGACC G^GT[ACGT]ACC
BstPI 551 GGTGACC G^GT[ACGT]ACC
BstXI 3584 CCACTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1610 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2444 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 551 GGTGACC G^GT[ACGT]ACC
EcoRI 858 GAATTC G^AATTC
Hin1I 1320 GACGTC G[GA]^CG[CT]C
MfeI 720 CAATTG C^AATTG
MunI 720 CAATTG C^AATTG
NheI 18 GCTAGC G^CTAGC
PflMI 1046 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 2694 GTTTAAAC GTTT^AAAC
PshBI 3752 ATTAAT AT^TAAT
PstI 1017 CTGCAG CTGCA^G
RcaI 2722 TCATGA T^CATGA
SnaBI 1930 TACGTA TAC^GTA
SspBI 2096 TGTACA T^GTACA
StuI 2270 AGGCCT AGG^CCT
Van91I 1046 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 935 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1047 CAGGCACTG
AspHI 1622 GAGCAC G[AT]GC[AT]^C
2748 GTGCTC
AvaII 960 GGACC G^G[AT]CC
3489 GGACC
BcnI 1369 CCGGG CC^[GC]GG
1653 CCCGG
BseRI 1494 CTCCTC GAGGAG(10/8)
191 GAGGAG
BsiHKAI 1622 GAGCAC G[AT]GC[AT]^C
2748 GTGCTC
BsmFI 1073 GGGAC GGGAC(10/14)
2968 GTCCC
DsaI 963 CCATGG C^C[GA][CT]GG
1069 CCATGG
EcoT22I 501 ATGCAT ATGCA^T
1670 ATGCAT
HapII 1369 CCGG C^CGG
1654 CCGG
HgaI 574 GACGC GACGC(5/10)
937 GCGTC
HpaII 1369 CCGG C^CGG
1654 CCGG
MspI 1369 CCGG C^CGG
1654 CCGG
NciI 1369 CCGGG CC^[GC]GG
1653 CCCGG
NcoI 963 CCATGG C^CATGG
1069 CCATGG
NsiI 501 ATGCAT ATGCA^T
1670 ATGCAT
PvuII 1020 CAGCTG CAG^CTG
1206 CAGCTG
XbaI 2675 TCTAGA T^CTAGA
3278 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BspLU11I BsrBI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI