##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
Aor51HI ApaI AscI Asp718I AspEI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BcgI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmFI
BspDI BspEI BspHI BspMI BsrBI
BssHII BssSI BstBI BstEII BstPI
BstUI CelII ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MaeII MluI MroI
MspA1I MvnI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
Tth111I Van91I XbaI XcmI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 2485 GTATAC GT^[AC][GT]AC
AflIII 3258 ACATGT A^C[GA][CT]GT
AlwI 2892 GATCC GGATC(4/5)
AvaII 15 GGACC G^G[AT]CC
BciVI 3957 GTATCC GTATCC(6/5)
BclI 190 TGATCA T^GATCA
BglI 2851 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2094 AGATCT A^GATCT
BsgI 937 CTGCAC GTGCAG(16/14)
BsmBI 2739 CGTCTC CGTCTC(1/5)
BspLU11I 3258 ACATGT A^CATGT
Bst1107I 2485 GTATAC GTA^TAC
BstAPI 3621 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2094 AGATCT [GA]^GATC[CT]
BstZ17I 2485 GTATAC GTA^TAC
Cfr13I 15 GGACC G^G[ACGT]CC
DraII 14 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 3649 CCATGG C^C[GA][CT]GG
EcoO109I 14 AGGACCC [GA]G^G[ACGT]CC[CT]
FauI 2911 CCCGC CCCGC(4/6)
FbaI 190 TGATCA T^GATCA
HgaI 8 GACGC GACGC(5/10)
HincII 44 GTTGAC GT[CT]^[GA]AC
HindII 44 GTTGAC GT[CT]^[GA]AC
MflI 2094 AGATCT [GA]^GATC[CT]
MslI 3068 CATTAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 3649 CCATGG C^CATGG
NdeI 2982 CATATG CA^TATG
NlaIV 15 GGACCC GG[ACGT]^[ACGT]CC
PaeR7I 2877 CTCGAG C^TCGAG
PpuMI 14 AGGACCC [GA]G^G[AT]CC[CT]
Sau96I 15 GGACC G^G[ACGT]CC
SexAI 151 ACCTGGT A^CC[AT]GGT
SfaNI 3132 GATGC GCATC(5/9)
SmaI 3613 CCCGGG CCC^GGG
StuI 1078 AGGCCT AGG^CCT
SwaI 3848 ATTTAAAT ATTT^AAAT
XhoI 2877 CTCGAG C^TCGAG
XhoII 2094 AGATCT [GA]^GATC[CT]
XmaCI 3613 CCCGGG C^CCGGG
XmaI 3613 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 408 GTGCAC G^TGCAC
1932 GTGCAC
AocI 2129 CCTGAGG CC^T[ACGT]AGG
3473 CCTGAGG
ApaLI 408 GTGCAC G^TGCAC
1932 GTGCAC
AseI 1922 ATTAAT AT^TAAT
1962 ATTAAT
AsnI 1922 ATTAAT AT^TAAT
1962 ATTAAT
Asp700I 545 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1117 GAATTCATTC
AspHI 408 GTGCAC G[AT]GC[AT]^C
1932 GTGCAC
BbsI 2041 GAAGAC GAAGAC(2/6)
156 GTCTTC
BbvCI 2767 CCTCAGC CCTCAGC(-5/-2)
3589 GCTGAGG
BbvI 171 GCTGC GCAGC(8/12)
2919 GCTGC
BpuAI 2041 GAAGAC GAAGAC(2/6)
156 GTCTTC
BsaBI 1921 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3081 GATGAAAATC
BsiHKAI 408 GTGCAC G[AT]GC[AT]^C
1932 GTGCAC
BsmI 3747 GAATGC GAATGC(1/-1)
3330 GCATTC
Bsp1407I 1893 TGTACA T^GTACA
4005 TGTACA
BsrFI 751 ACCGGC [GA]^CCGG[CT]
1255 GCCGGT
BsrGI 1893 TGTACA T^GTACA
4005 TGTACA
BstXI 2777 CCAAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3440 CCAGCACTTTGG
Bsu36I 2129 CCTGAGG CC^T[ACGT]AGG
3473 CCTGAGG
Cfr10I 751 ACCGGC [GA]^CCGG[CT]
1255 GCCGGT
Eco81I 2129 CCTGAGG CC^T[ACGT]AGG
3473 CCTGAGG
EcoT14I 219 CCTTGG C^C[AT][AT]GG
3649 CCATGG
EcoT22I 3075 ATGCAT ATGCA^T
3133 ATGCAT
Fnu4HI 171 GCTGC GC^[ACGT]GC
2919 GCTGC
ItaI 171 GCTGC GC^[ACGT]GC
2919 GCTGC
MamI 1921 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3081 GATGAAAATC
MfeI 2136 CAATTG C^AATTG
4001 CAATTG
MunI 2136 CAATTG C^AATTG
4001 CAATTG
MwoI 2851 GCCATGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
3621 GCAGAGGTTGC
NsiI 3075 ATGCAT ATGCA^T
3133 ATGCAT
PshBI 1922 ATTAAT AT^TAAT
1962 ATTAAT
ScaI 3122 AGTACT AGT^ACT
3765 AGTACT
SphI 583 GCATGC GCATG^C
3560 GCATGC
SspBI 1893 TGTACA T^GTACA
4005 TGTACA
StyI 219 CCTTGG C^C[AT][AT]GG
3649 CCATGG
TseI 171 GCTGC G^C[AT]GC
2919 GCTGC
XmnI 545 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1117 GAATTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaI BalI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI DdeI DpnII DraI EaeI
EarI EcoRI EcoRII FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MspI MvaI NciI NdeII
NlaIII NspI PleI RsaI Sau3AI
ScrFI SfcI SmlI SspI TaqI
TfiI Tru9I Tsp45I Tsp509I TspRI
TthHB8I