##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AgeI AscI
AseI AsnI Asp718I AspI BamHI
BbrPI BcgI BciVI BclI BglII
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrGI BssHII BssSI BstBI ClaI
CpoI DrdI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MluI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SspBI
SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AfaI 474 GTAC GT^AC
AflII 3682 CTTAAG C^TTAAG
AflIII 15 ACGTGT A^C[GA][CT]GT
Alw44I 550 GTGCAC G^TGCAC
AocI 4529 CCTGAGG CC^T[ACGT]AGG
Aor51HI 413 AGCGCT AGC^GCT
ApaLI 550 GTGCAC G^TGCAC
AviII 1509 TGCGCA TGC^GCA
BbsI 3304 GAAGAC GAAGAC(2/6)
BfrI 3682 CTTAAG C^TTAAG
BpuAI 3304 GAAGAC GAAGAC(2/6)
BsaBI 184 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 1296 TCATGA T^CATGA
BsrBI 860 CCGCTC CCGCTC(-3/-3)
BsrDI 3769 CATTGC GCAATG(2/0)
Bst1107I 1542 GTATAC GTA^TAC
BstZ17I 1542 GTATAC GTA^TAC
Bsu36I 4529 CCTGAGG CC^T[ACGT]AGG
Eco47III 413 AGCGCT AGC^GCT
Eco81I 4529 CCTGAGG CC^T[ACGT]AGG
FspI 1509 TGCGCA TGC^GCA
HincII 4524 GTCGAC GT[CT]^[GA]AC
HindII 4524 GTCGAC GT[CT]^[GA]AC
KspI 81 CCGCGG CCGC^GG
MamI 184 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1289 CAATTG C^AATTG
MslI 991 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1289 CAATTG C^AATTG
NaeI 71 GCCGGC GCC^GGC
NgoMIV 71 GCCGGC G^CCGGC
NspI 1468 ACATGC [GA]CATG^[CT]
PvuI 1178 CGATCG CGAT^CG
RcaI 1296 TCATGA T^CATGA
RsaI 474 GTAC GT^AC
SacII 81 CCGCGG CCGC^GG
SalI 4524 GTCGAC G^TCGAC
XbaI 118 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 4402 GAATTC [GA]^AATT[CT]
4410 GAATTC
AhdI 784 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1943 GACCCTCGGTC
ApoI 4402 GAATTC [GA]^AATT[CT]
4410 GAATTC
Asp700I 720 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4244 GAAAGCCTTC
AspEI 784 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1943 GACCCTCGGTC
AvrII 2491 CCTAGG C^CTAGG
4190 CCTAGG
BlnI 2491 CCTAGG C^CTAGG
4190 CCTAGG
BlpI 40 GCTGAGC GC^T[ACGT]AGC
2929 GCTCAGC
Bpu1102I 40 GCTGAGC GC^T[ACGT]AGC
2929 GCTCAGC
BsaAI 547 TACGTG [CT]AC^GT[GA]
2281 TACGTG
BsaI 1941 GAGACC GGTCTC(1/5)
2707 GGTCTC
BsmBI 3745 CGTCTC CGTCTC(1/5)
745 GAGACG
BstAPI 262 GCAATCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2230 GCAGACCCTGC
CelII 40 GCTGAGC GC^T[ACGT]AGC
2929 GCTCAGC
DraI 3532 TTTAAA TTT^AAA
4688 TTTAAA
DraIII 1857 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2242 CACACGGTG
EagI 843 CGGCCG C^GGCCG
2029 CGGCCG
Eam1105I 784 GACGGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1943 GACCCTCGGTC
EclXI 843 CGGCCG C^GGCCG
2029 CGGCCG
Eco52I 843 CGGCCG C^GGCCG
2029 CGGCCG
EcoNI 2414 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3214 CCTCAGTAAGG
EcoRI 4402 GAATTC G^AATTC
4410 GAATTC
NcoI 990 CCATGG C^CATGG
1955 CCATGG
SacI 1933 GAGCTC GAGCT^C
2367 GAGCTC
SbfI 2218 CCTGCAGG CCTGCA^GG
3187 CCTGCAGG
Sse8387I 2218 CCTGCAGG CCTGCA^GG
3187 CCTGCAGG
StuI 2665 AGGCCT AGG^CCT
3634 AGGCCT
XmnI 720 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4244 GAAAGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AccIII AciI AcyI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI