################################## # NO CUT ENZYMES ( 63 enzymes) # ################################## Acc65I AclI AcsI AflII AgeI AocI ApaI ApoI AscI Asp700I Asp718I AviII AvrII BamHI BbrPI BciVI BfrI BglII BlnI BsiWI BspDI BsrDI Bst1107I BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco81I EcoRI FseI FspI KpnI MfeI MluI MunI NaeI NgoMIV NheI NotI NruI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SexAI SgrAI SmaI SnaBI SpeI SspI StuI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AccIII 3197 TCCGGA T^CCGGA AhdI 213 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 687 GTGCAC G^TGCAC ApaLI 687 GTGCAC G^TGCAC AseI 3653 ATTAAT AT^TAAT AsnI 3653 ATTAAT AT^TAAT AspEI 213 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 17 GGCGCC GGCGC^C BbvCI 2640 GCTGAGG CCTCAGC(-5/-2) BcgI 1936 GCAGCGAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 877 TGATCA T^GATCA BglI 2277 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2049 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2049 GCTGAGC GC^T[ACGT]AGC BsaAI 2963 CACGTA [CT]AC^GT[GA] BsaBI 2792 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3197 TCCGGA [AT]^CCGG[AT] BseAI 3197 TCCGGA T^CCGGA BsmI 2519 GAATGC GAATGC(1/-1) BspEI 3197 TCCGGA T^CCGGA BspHI 2179 TCATGA T^CATGA BsrBI 2787 GAGCGG CCGCTC(-3/-3) BssHII 26 GCGCGC G^CGCGC BssSI 3580 CACGAG CACGAG(-5/-1) BstAPI 3124 GCAAACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2866 TTCGAA TT^CGAA BstEII 1235 GGTCACC G^GT[ACGT]ACC BstPI 1235 GGTCACC G^GT[ACGT]ACC BstYI 1147 AGATCC [GA]^GATC[CT] CelII 2049 GCTGAGC GC^T[ACGT]AGC EagI 184 CGGCCG C^GGCCG Eam1105I 213 GACAGCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 184 CGGCCG C^GGCCG Eco52I 184 CGGCCG C^GGCCG EcoNI 2192 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1235 GGTCACC G^GT[ACGT]ACC EcoRV 1557 GATATC GAT^ATC FbaI 877 TGATCA T^GATCA HpaI 2151 GTTAAC GTT^AAC KasI 17 GGCGCC G^GCGCC KspI 2016 CCGCGG CCGC^GG MamI 2792 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1147 AGATCC [GA]^GATC[CT] MroI 3197 TCCGGA T^CCGGA NarI 17 GGCGCC GG^CGCC NdeI 873 CATATG CA^TATG NspV 2866 TTCGAA TT^CGAA PacI 3541 TTAATTAA TTAAT^TAA PaeR7I 3587 CTCGAG C^TCGAG PflFI 323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 3653 ATTAAT AT^TAAT RcaI 2179 TCATGA T^CATGA SacII 2016 CCGCGG CCGC^GG SapI 1700 GAAGAGC GCTCTTC(1/4) ScaI 3632 AGTACT AGT^ACT SfiI 2276 GGCCGCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 17 GGCGCC GGC^GCC SfuI 2866 TTCGAA TT^CGAA SmlI 3587 CTCGAG C^T[CT][GA]AG Tth111I 323 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 3587 CTCGAG C^TCGAG XhoII 1147 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AatII 1790 GACGTC GACGT^C 3083 GACGTC AccI 1416 GTCGAC GT^[AC][GT]AC 2648 GTCGAC AlwI 1148 GATCC GGATC(4/5) 2708 GATCC Aor51HI 654 AGCGCT AGC^GCT 751 AGCGCT AvaI 3093 CCCGAG C^[CT]CG[GA]G 3587 CTCGAG BsgI 408 CTGCAC GTGCAG(16/14) 576 GTGCAG BsiEI 184 CGGCCG CG[GA][CT]^CG 2327 CGACCG BsmBI 699 GAGACG CGTCTC(1/5) 1275 GAGACG BsoBI 3093 CCCGAG C^[CT]CG[GA]G 3587 CTCGAG Bsp1407I 358 TGTACA T^GTACA 3388 TGTACA BspLU11I 130 ACATGT A^CATGT 3332 ACATGT BsrFI 586 ACCGGC [GA]^CCGG[CT] 2054 GCCGGT BsrGI 358 TGTACA T^GTACA 3388 TGTACA BstXI 1097 CCACCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1954 CCATCAAGATGG Cfr10I 586 ACCGGC [GA]^CCGG[CT] 2054 GCCGGT DraII 156 AGGGCCT [GA]G^G[ACGT]CC[CT] 3304 AGGCCCC Eco47III 654 AGCGCT AGC^GCT 751 AGCGCT EcoO109I 156 AGGGCCT [GA]G^G[ACGT]CC[CT] 3304 AGGCCCC HindIII 964 AAGCTT A^AGCTT 1896 AAGCTT NcoI 508 CCATGG C^CATGG 1822 CCATGG PshAI 1277 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1763 GACCCTGGTC SalI 1416 GTCGAC G^TCGAC 2648 GTCGAC SbfI 854 CCTGCAGG CCTGCA^GG 1187 CCTGCAGG Sse8387I 854 CCTGCAGG CCTGCA^GG 1187 CCTGCAGG SspBI 358 TGTACA T^GTACA 3388 TGTACA Tsp509I 3543 AATT ^AATT 3656 AATT XcmI 238 CCAGTGACCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1822 CCATGGAGGAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwNI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I