##################################
# NO CUT ENZYMES (   63 enzymes) #
##################################

Acc65I      AclI        AcsI        AflII       AgeI        
AocI        ApaI        ApoI        AscI        Asp700I     
Asp718I     AviII       AvrII       BamHI       BbrPI       
BciVI       BfrI        BglII       BlnI        BsiWI       
BspDI       BsrDI       Bst1107I    BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eco81I      EcoRI       FseI        FspI        KpnI        
MfeI        MluI        MunI        NaeI        NgoMIV      
NheI        NotI        NruI        PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PvuI        
RsrII       SexAI       SgrAI       SmaI        SnaBI       
SpeI        SspI        StuI        SwaI        XbaI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

AccIII       3197  TCCGGA               T^CCGGA
AhdI          213  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        687  GTGCAC               G^TGCAC
ApaLI         687  GTGCAC               G^TGCAC
AseI         3653  ATTAAT               AT^TAAT
AsnI         3653  ATTAAT               AT^TAAT
AspEI         213  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          323  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbeI           17  GGCGCC               GGCGC^C
BbvCI        2640  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1936  GCAGCGAAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          877  TGATCA               T^GATCA
BglI         2277  GCCGCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI         2049  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2049  GCTGAGC              GC^T[ACGT]AGC
BsaAI        2963  CACGTA               [CT]AC^GT[GA]
BsaBI        2792  GATGCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        3197  TCCGGA               [AT]^CCGG[AT]
BseAI        3197  TCCGGA               T^CCGGA
BsmI         2519  GAATGC               GAATGC(1/-1)
BspEI        3197  TCCGGA               T^CCGGA
BspHI        2179  TCATGA               T^CATGA
BsrBI        2787  GAGCGG               CCGCTC(-3/-3)
BssHII         26  GCGCGC               G^CGCGC
BssSI        3580  CACGAG               CACGAG(-5/-1)
BstAPI       3124  GCAAACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2866  TTCGAA               TT^CGAA
BstEII       1235  GGTCACC              G^GT[ACGT]ACC
BstPI        1235  GGTCACC              G^GT[ACGT]ACC
BstYI        1147  AGATCC               [GA]^GATC[CT]
CelII        2049  GCTGAGC              GC^T[ACGT]AGC
EagI          184  CGGCCG               C^GGCCG
Eam1105I      213  GACAGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         184  CGGCCG               C^GGCCG
Eco52I        184  CGGCCG               C^GGCCG
EcoNI        2192  CCTGGGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1235  GGTCACC              G^GT[ACGT]ACC
EcoRV        1557  GATATC               GAT^ATC
FbaI          877  TGATCA               T^GATCA
HpaI         2151  GTTAAC               GTT^AAC
KasI           17  GGCGCC               G^GCGCC
KspI         2016  CCGCGG               CCGC^GG
MamI         2792  GATGCATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI         1147  AGATCC               [GA]^GATC[CT]
MroI         3197  TCCGGA               T^CCGGA
NarI           17  GGCGCC               GG^CGCC
NdeI          873  CATATG               CA^TATG
NspV         2866  TTCGAA               TT^CGAA
PacI         3541  TTAATTAA             TTAAT^TAA
PaeR7I       3587  CTCGAG               C^TCGAG
PflFI         323  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        3653  ATTAAT               AT^TAAT
RcaI         2179  TCATGA               T^CATGA
SacII        2016  CCGCGG               CCGC^GG
SapI         1700  GAAGAGC              GCTCTTC(1/4)
ScaI         3632  AGTACT               AGT^ACT
SfiI         2276  GGCCGCTGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI           17  GGCGCC               GGC^GCC
SfuI         2866  TTCGAA               TT^CGAA
SmlI         3587  CTCGAG               C^T[CT][GA]AG
Tth111I       323  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3587  CTCGAG               C^TCGAG
XhoII        1147  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AatII        1790  GACGTC               GACGT^C
             3083  GACGTC              
AccI         1416  GTCGAC               GT^[AC][GT]AC
             2648  GTCGAC              
AlwI         1148  GATCC                GGATC(4/5)
             2708  GATCC               
Aor51HI       654  AGCGCT               AGC^GCT
              751  AGCGCT              
AvaI         3093  CCCGAG               C^[CT]CG[GA]G
             3587  CTCGAG              
BsgI          408  CTGCAC               GTGCAG(16/14)
              576  GTGCAG              
BsiEI         184  CGGCCG               CG[GA][CT]^CG
             2327  CGACCG              
BsmBI         699  GAGACG               CGTCTC(1/5)
             1275  GAGACG              
BsoBI        3093  CCCGAG               C^[CT]CG[GA]G
             3587  CTCGAG              
Bsp1407I      358  TGTACA               T^GTACA
             3388  TGTACA              
BspLU11I      130  ACATGT               A^CATGT
             3332  ACATGT              
BsrFI         586  ACCGGC               [GA]^CCGG[CT]
             2054  GCCGGT              
BsrGI         358  TGTACA               T^GTACA
             3388  TGTACA              
BstXI        1097  CCACCAGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1954  CCATCAAGATGG        
Cfr10I        586  ACCGGC               [GA]^CCGG[CT]
             2054  GCCGGT              
DraII         156  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             3304  AGGCCCC             
Eco47III      654  AGCGCT               AGC^GCT
              751  AGCGCT              
EcoO109I      156  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             3304  AGGCCCC             
HindIII       964  AAGCTT               A^AGCTT
             1896  AAGCTT              
NcoI          508  CCATGG               C^CATGG
             1822  CCATGG              
PshAI        1277  GACGCCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1763  GACCCTGGTC          
SalI         1416  GTCGAC               G^TCGAC
             2648  GTCGAC              
SbfI          854  CCTGCAGG             CCTGCA^GG
             1187  CCTGCAGG            
Sse8387I      854  CCTGCAGG             CCTGCA^GG
             1187  CCTGCAGG            
SspBI         358  TGTACA               T^GTACA
             3388  TGTACA              
Tsp509I      3543  AATT                 ^AATT
             3656  AATT                
XcmI          238  CCAGTGACCGAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1822  CCATGGAGGAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AflIII      
AluI        AlwNI       AspHI       AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoRII      EcoT14I     
EcoT22I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NsiI        NspI        PflMI       
PleI        PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SphI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     Van91I