##################################
# NO CUT ENZYMES (   76 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AgeI        
AscI        AviII       AvrII       BbeI        BbrPI       
BciVI       BclI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BseAI       BsiWI       BsmBI       
BspDI       BspEI       BspHI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DraIII      
DrdI        EcoNI       EcoO65I     EcoRV       FbaI        
FspI        HpaI        KasI        MamI        MluI        
MroI        NarI        NcoI        NdeI        NotI        
NruI        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SnaBI       
SpeI        Sse8387I    StuI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I       2412  GGTACC               G^GTACC
AhdI          607  GACGCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2846  GTGCAC               G^TGCAC
AocI         1422  CCTCAGG              CC^T[ACGT]AGG
ApaLI        2846  GTGCAC               G^TGCAC
Asp700I      1085  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      2412  GGTACC               G^GTACC
AspEI         607  GACGCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2721  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         2905  TGGCCA               TGG^CCA
BbvCI        2814  CCTCAGC              CCTCAGC(-5/-2)
BglII        1645  AGATCT               A^GATCT
BsaWI         164  TCCGGT               [AT]^CCGG[AT]
Bsp1407I     3808  TGTACA               T^GTACA
BspLU11I     3025  ACATGT               A^CATGT
BsrBI         602  GAGCGG               CCGCTC(-3/-3)
BsrGI        3808  TGTACA               T^GTACA
BstBI        1364  TTCGAA               TT^CGAA
Bsu36I       1422  CCTCAGG              CC^T[ACGT]AGG
Eam1105I      607  GACGCCTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          905  GAAGAG               CTCTTC(1/4)
Eco81I       1422  CCTCAGG              CC^T[ACGT]AGG
EcoT22I      3965  ATGCAT               ATGCA^T
FseI          442  GGCCGGCC             GGCCGG^CC
HincII       4043  GTCAAC               GT[CT]^[GA]AC
HindII       4043  GTCAAC               GT[CT]^[GA]AC
KpnI         2412  GGTACC               GGTAC^C
Ksp632I       905  GAAGAG               CTCTTC(1/4)
KspI          665  CCGCGG               CCGC^GG
MluNI        2905  TGGCCA               TGG^CCA
MscI         2905  TGGCCA               TGG^CCA
NaeI          443  GCCGGC               GCC^GGC
NgoMIV        443  GCCGGC               G^CCGGC
NheI         1541  GCTAGC               G^CTAGC
NsiI         3965  ATGCAT               ATGCA^T
NspV         1364  TTCGAA               TT^CGAA
PflFI        2721  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3820  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        3088  AGGACCC              [GA]G^G[AT]CC[CT]
SacII         665  CCGCGG               CCGC^GG
SapI          905  GAAGAGC              GCTCTTC(1/4)
SfuI         1364  TTCGAA               TT^CGAA
SmaI          671  CCCGGG               CCC^GGG
SphI         2010  GCATGC               GCATG^C
SspBI        3808  TGTACA               T^GTACA
Tth111I      2721  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3820  CCACTTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         3709  CCAAAAAATATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         671  CCCGGG               C^CCGGG
XmaI          671  CCCGGG               C^CCGGG
XmnI         1085  GAAAAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AcyI          607  GACGCC               G[GA]^CG[CT]C
             1009  GGCGTC              
AflII        2522  CTTAAG               C^TTAAG
             3621  CTTAAG              
AflIII       2357  ACACGT               A^C[GA][CT]GT
             3025  ACATGT              
AlwNI        1122  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1352  CAGTTTCTG           
Aor51HI      1451  AGCGCT               AGC^GCT
             3771  AGCGCT              
ApaI          247  GGGCCC               GGGCC^C
             2544  GGGCCC              
AseI         2552  ATTAAT               AT^TAAT
             3686  ATTAAT              
AsnI         2552  ATTAAT               AT^TAAT
             3686  ATTAAT              
AspHI        2846  GTGCAC               G[AT]GC[AT]^C
             3958  GTGCTC              
BamHI        1860  GGATCC               G^GATCC
             3678  GGATCC              
BbsI          177  GAAGAC               GAAGAC(2/6)
              956  GAAGAC              
BcgI         2803  CGATTCTCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3947  GCATGTAGTTCG        
BfrI         2522  CTTAAG               C^TTAAG
             3621  CTTAAG              
BglI          661  GCCACCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              713  GCCACGGGGGC         
BpuAI         177  GAAGAC               GAAGAC(2/6)
              956  GAAGAC              
BsaHI         607  GACGCC               G[GA]^CG[CT]C
             1009  GGCGTC              
BsaI         2915  GGTCTC               GGTCTC(1/5)
             3442  GGTCTC              
BsiEI         441  CGGCCG               CG[GA][CT]^CG
              588  CGGCCG              
BsiHKAI      2846  GTGCAC               G[AT]GC[AT]^C
             3958  GTGCTC              
BspMI         264  ACCTGC               ACCTGC(4/8)
              428  GCAGGT              
BsrFI         443  GCCGGC               [GA]^CCGG[CT]
              625  GCCGGT              
Cfr10I        443  GCCGGC               [GA]^CCGG[CT]
              625  GCCGGT              
DsaI          665  CCGCGG               C^C[GA][CT]GG
              714  CCACGG              
EagI          441  CGGCCG               C^GGCCG
              588  CGGCCG              
EclXI         441  CGGCCG               C^GGCCG
              588  CGGCCG              
Eco47III     1451  AGCGCT               AGC^GCT
             3771  AGCGCT              
Eco52I        441  CGGCCG               C^GGCCG
              588  CGGCCG              
HaeII        1451  AGCGCT               [GA]GCGC^[CT]
             3771  AGCGCT              
Hin1I         607  GACGCC               G[GA]^CG[CT]C
             1009  GGCGTC              
HindIII       929  AAGCTT               A^AGCTT
             1088  AAGCTT              
MfeI         2271  CAATTG               C^AATTG
             3334  CAATTG              
MunI         2271  CAATTG               C^AATTG
             3334  CAATTG              
PshBI        2552  ATTAAT               AT^TAAT
             3686  ATTAAT              
PvuII          66  CAGCTG               CAG^CTG
             1578  CAGCTG              
ScaI         2390  AGTACT               AGT^ACT
             3761  AGTACT              


#########################################
# 3 OR MORE CUT ENZYMES (   96 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII