##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AccIII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AspEI AspI
AvaI AviII AvrII BalI BbeI
BbrPI BcnI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BsgI BsiEI
BsoBI Bsp1407I BspEI BspLU11I BsrGI
BssHII BssSI BstEII BstPI CelII
CpoI DraII DraIII DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I FseI FspI
HaeII HgaI HincII HindII HpaI
KasI KspI MluI MluNI MroI
MscI MspA1I NarI NciI NcoI
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 1811 GACGTC GACGT^C
AclI 2829 AACGTT AA^CGTT
AcyI 1811 GACGTC G[GA]^CG[CT]C
AflII 788 CTTAAG C^TTAAG
AlwNI 32 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 772 CCTCAGG CC^T[ACGT]AGG
AseI 2694 ATTAAT AT^TAAT
AsnI 2694 ATTAAT AT^TAAT
AspHI 2734 GAGCTC G[AT]GC[AT]^C
AvaII 1341 GGACC G^G[AT]CC
BamHI 2047 GGATCC G^GATCC
BbvCI 1511 GCTGAGG CCTCAGC(-5/-2)
BcgI 117 GCAAAAATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 293 GGATAC GTATCC(6/5)
BfrI 788 CTTAAG C^TTAAG
BglI 598 GCCGACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1656 AGATCT A^GATCT
BpmI 1099 CTGGAG CTGGAG(16/14)
BsaBI 1404 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1811 GACGTC G[GA]^CG[CT]C
BseRI 2167 GAGGAG GAGGAG(10/8)
BsiHKAI 2734 GAGCTC G[AT]GC[AT]^C
BsiWI 1718 CGTACG C^GTACG
BspDI 125 ATCGAT AT^CGAT
BspMI 1985 ACCTGC ACCTGC(4/8)
BsrBI 13 GAGCGG CCGCTC(-3/-3)
BsrDI 268 GCAATG GCAATG(2/0)
BsrFI 405 GCCGGC [GA]^CCGG[CT]
Bst1107I 2199 GTATAC GTA^TAC
BstBI 591 TTCGAA TT^CGAA
BstZ17I 2199 GTATAC GTA^TAC
Bsu36I 772 CCTCAGG CC^T[ACGT]AGG
Cfr10I 405 GCCGGC [GA]^CCGG[CT]
ClaI 125 ATCGAT AT^CGAT
Eco81I 772 CCTCAGG CC^T[ACGT]AGG
EcoRI 3111 GAATTC G^AATTC
EcoRV 699 GATATC GAT^ATC
FauI 15 GCGGG CCCGC(4/6)
Hin1I 1811 GACGTC G[GA]^CG[CT]C
MamI 1404 GATCCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 405 GCCGGC GCC^GGC
NdeI 2658 CATATG CA^TATG
NgoMIV 405 GCCGGC G^CCGGC
NspV 591 TTCGAA TT^CGAA
PflMI 2588 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1861 GACTC GAGTC(4/5)
PshBI 2694 ATTAAT AT^TAAT
Psp1406I 2829 AACGTT AA^CGTT
SacI 2734 GAGCTC GAGCT^C
SexAI 3255 ACCTGGT A^CC[AT]GGT
SfuI 591 TTCGAA TT^CGAA
Van91I 2588 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 396 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 1140 GGTACC G^GTACC
3060 GGTACC
AccI 2011 GTCTAC GT^[AC][GT]AC
2199 GTATAC
Asp700I 828 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3107 GAAGGAATTC
Asp718I 1140 GGTACC G^GTACC
3060 GGTACC
BbsI 3329 GAAGAC GAAGAC(2/6)
1700 GTCTTC
BclI 1415 TGATCA T^GATCA
1601 TGATCA
BpuAI 3329 GAAGAC GAAGAC(2/6)
1700 GTCTTC
BsaAI 1720 TACGTG [CT]AC^GT[GA]
3163 TACGTG
BspHI 2450 TCATGA T^CATGA
3033 TCATGA
BstAPI 31 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2302 GCATCACTTGC
BstXI 1569 CCAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2312 CCAAACTGTTGG
BstYI 1656 AGATCT [GA]^GATC[CT]
2047 GGATCC
CfoI 21 GCGC GCG^C
614 GCGC
Cfr13I 777 GGCCC G^G[ACGT]CC
1341 GGACC
DrdI 168 GACTTTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
264 GACAGCAATGTC
EcoT14I 645 CCTTGG C^C[AT][AT]GG
3056 CCAAGG
EcoT22I 547 ATGCAT ATGCA^T
3631 ATGCAT
FbaI 1415 TGATCA T^GATCA
1601 TGATCA
HhaI 21 GCGC GCG^C
614 GCGC
HinP1I 21 GCGC G^CGC
614 GCGC
KpnI 1140 GGTACC GGTAC^C
3060 GGTACC
MfeI 2052 CAATTG C^AATTG
3620 CAATTG
MflI 1656 AGATCT [GA]^GATC[CT]
2047 GGATCC
MunI 2052 CAATTG C^AATTG
3620 CAATTG
NsiI 547 ATGCAT ATGCA^T
3631 ATGCAT
RcaI 2450 TCATGA T^CATGA
3033 TCATGA
SapI 862 GCTCTTC GCTCTTC(1/4)
4084 GCTCTTC
Sau96I 777 GGCCC G^G[ACGT]CC
1341 GGACC
SfaNI 2302 GCATC GCATC(5/9)
3143 GCATC
StuI 642 AGGCCT AGG^CCT
907 AGGCCT
StyI 645 CCTTGG C^C[AT][AT]GG
3056 CCAAGG
Tsp45I 722 GTCAC ^GT[GC]AC
1814 GTCAC
XhoII 1656 AGATCT [GA]^GATC[CT]
2047 GGATCC
XmnI 828 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3107 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI BanI BanII
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I DdeI DpnII DraI
EarI EcoRII Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
ScrFI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I