##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BglI BglII
BlnI BsaI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI FseI FspI
HgaI HpaI KpnI KspI MluI
MroI NaeI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AflII 3357 CTTAAG C^TTAAG
ApaI 171 GGGCCC GGGCC^C
Asp700I 556 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 3537 GAAGAC GAAGAC(2/6)
BbvCI 176 CCTCAGC CCTCAGC(-5/-2)
BciVI 2694 GTATCC GTATCC(6/5)
BclI 3287 TGATCA T^GATCA
BfrI 3357 CTTAAG C^TTAAG
BlpI 270 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 270 GCTGAGC GC^T[ACGT]AGC
BpuAI 3537 GAAGAC GAAGAC(2/6)
BsaAI 2991 TACGTG [CT]AC^GT[GA]
BsmBI 95 GAGACG CGTCTC(1/5)
BspHI 333 TCATGA T^CATGA
BspMI 302 ACCTGC ACCTGC(4/8)
BssSI 190 CACGAG CACGAG(-5/-1)
BstXI 1155 CCAGTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3635 GGATCT [GA]^GATC[CT]
CelII 270 GCTGAGC GC^T[ACGT]AGC
DsaI 1577 CCACGG C^C[GA][CT]GG
EcoNI 303 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3717 GATATC GAT^ATC
FbaI 3287 TGATCA T^GATCA
HincII 2044 GTTGAC GT[CT]^[GA]AC
HindII 2044 GTTGAC GT[CT]^[GA]AC
HindIII 1653 AAGCTT A^AGCTT
MfeI 2295 CAATTG C^AATTG
MflI 3635 GGATCT [GA]^GATC[CT]
MunI 2295 CAATTG C^AATTG
PmeI 1115 GTTTAAAC GTTT^AAAC
RcaI 333 TCATGA T^CATGA
SacI 754 GAGCTC GAGCT^C
SapI 814 GAAGAGC GCTCTTC(1/4)
SmlI 3357 CTTAAG C^T[CT][GA]AG
SpeI 3250 ACTAGT A^CTAGT
XcmI 1577 CCACGGTGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3635 GGATCT [GA]^GATC[CT]
XmnI 556 GAACTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AcyI 66 GGCGCC G[GA]^CG[CT]C
196 GGCGCC
AflIII 1050 ACGTGT A^C[GA][CT]GT
2992 ACGTGT
AlwI 3118 GATCC GGATC(4/5)
3635 GGATC
AocI 229 CCTGAGG CC^T[ACGT]AGG
2139 CCTAAGG
AseI 1302 ATTAAT AT^TAAT
3615 ATTAAT
AsnI 1302 ATTAAT AT^TAAT
3615 ATTAAT
AvaI 1 CCCGGG C^[CT]CG[GA]G
70 CCCGGG
BalI 615 TGGCCA TGG^CCA
3095 TGGCCA
BanI 66 GGCGCC G^G[CT][GA]CC
196 GGCGCC
BbeI 66 GGCGCC GGCGC^C
196 GGCGCC
BsaBI 733 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3636 GATCTCAATC
BsaHI 66 GGCGCC G[GA]^CG[CT]C
196 GGCGCC
BsoBI 1 CCCGGG C^[CT]CG[GA]G
70 CCCGGG
BsrDI 1524 CATTGC GCAATG(2/0)
1932 CATTGC
Bst1107I 2107 GTATAC GTA^TAC
3859 GTATAC
BstAPI 252 GCAGCGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
712 GCACTCTTTGC
BstZ17I 2107 GTATAC GTA^TAC
3859 GTATAC
Bsu36I 229 CCTGAGG CC^T[ACGT]AGG
2139 CCTAAGG
DrdI 472 GACTTAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2678 GACAGTGATGTC
EaeI 615 TGGCCA [CT]^GGCC[GA]
3095 TGGCCA
Eco81I 229 CCTGAGG CC^T[ACGT]AGG
2139 CCTAAGG
EcoT14I 2689 CCAAGG C^C[AT][AT]GG
3542 CCTTGG
Hin1I 66 GGCGCC G[GA]^CG[CT]C
196 GGCGCC
KasI 66 GGCGCC G^GCGCC
196 GGCGCC
MamI 733 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3636 GATCTCAATC
MluNI 615 TGGCCA TGG^CCA
3095 TGGCCA
MscI 615 TGGCCA TGG^CCA
3095 TGGCCA
NarI 66 GGCGCC GG^CGCC
196 GGCGCC
PflMI 1945 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2020 CCAGAAGGTGG
PshBI 1302 ATTAAT AT^TAAT
3615 ATTAAT
PstI 250 CTGCAG CTGCA^G
2150 CTGCAG
PvuII 1361 CAGCTG CAG^CTG
1484 CAGCTG
SfoI 66 GGCGCC GGC^GCC
196 GGCGCC
SmaI 1 CCCGGG CCC^GGG
70 CCCGGG
StyI 2689 CCAAGG C^C[AT][AT]GG
3542 CCTTGG
Van91I 1945 CCACCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2020 CCAGAAGGTGG
XbaI 3576 TCTAGA T^CTAGA
3740 TCTAGA
XmaCI 1 CCCGGG C^CCGGG
70 CCCGGG
XmaI 1 CCCGGG C^CCGGG
70 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwNI ApoI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeI
NdeII NlaIII NlaIV NsiI NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SspI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI