##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
AspEI       AspHI       AvaI        AviII       AvrII       
BanII       BbeI        BbrPI       BcgI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaHI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsoBI       BspDI       
BspEI       BspHI       BssHII      BssSI       Bst1107I    
BstAPI      BstUI       BstZ17I     CelII       ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoRI       
EcoRV       FauI        FseI        FspI        HaeII       
HgaI        Hin1I       KasI        KspI        MluI        
MroI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SpeI        SphI        
Sse8387I    XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

Acc65I       3960  GGTACC               G^GTACC
AccI         2898  GTAGAC               GT^[AC][GT]AC
AflIII       1096  ACATGT               A^C[GA][CT]GT
AocI         1254  CCTTAGG              CC^T[ACGT]AGG
Asp700I      1434  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      3960  GGTACC               G^GTACC
AspI          428  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1898  TGGCCA               TGG^CCA
BciVI        2316  GGATAC               GTATCC(6/5)
BglII        2654  AGATCT               A^GATCT
BmyI         2672  GTGCCC               G[AGT]GC[ACT]^C
BsaAI        1553  TACGTA               [CT]AC^GT[GA]
BsaI         2684  GAGACC               GGTCTC(1/5)
BsgI         3194  GTGCAG               GTGCAG(16/14)
Bsp1286I     2672  GTGCCC               G[AGT]GC[ACT]^C
Bsp1407I     2834  TGTACA               T^GTACA
BspLU11I     1096  ACATGT               A^CATGT
BsrBI        1598  GAGCGG               CCGCTC(-3/-3)
BsrFI         808  GCCGGT               [GA]^CCGG[CT]
BsrGI        2834  TGTACA               T^GTACA
BstBI        3247  TTCGAA               TT^CGAA
BstEII       1013  GGTGACC              G^GT[ACGT]ACC
BstPI        1013  GGTGACC              G^GT[ACGT]ACC
Bsu36I       1254  CCTTAGG              CC^T[ACGT]AGG
CfoI         4213  GCGC                 GCG^C
Cfr10I        808  GCCGGT               [GA]^CCGG[CT]
DraII        4033  AGGACCT              [GA]G^G[ACGT]CC[CT]
DsaI         1372  CCGTGG               C^C[GA][CT]GG
EaeI         1898  TGGCCA               [CT]^GGCC[GA]
Eco81I       1254  CCTTAGG              CC^T[ACGT]AGG
EcoO109I     4033  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoO65I      1013  GGTGACC              G^GT[ACGT]ACC
HapII         809  CCGG                 C^CGG
HhaI         4213  GCGC                 GCG^C
HinP1I       4213  GCGC                 G^CGC
HpaII         809  CCGG                 C^CGG
KpnI         3960  GGTACC               GGTAC^C
MluNI        1898  TGGCCA               TGG^CCA
MscI         1898  TGGCCA               TGG^CCA
MspI          809  CCGG                 C^CGG
NspI         1096  ACATGT               [GA]CATG^[CT]
NspV         3247  TTCGAA               TT^CGAA
PacI         3023  TTAATTAA             TTAAT^TAA
PflFI         428  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        4033  AGGACCT              [GA]G^G[AT]CC[CT]
SfuI         3247  TTCGAA               TT^CGAA
SnaBI        1553  TACGTA               TAC^GTA
SspBI        2834  TGTACA               T^GTACA
StuI         3217  AGGCCT               AGG^CCT
Tth111I       428  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI         1434  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AlwNI         924  CAGTACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3444  CAGTGCCTG           
BamHI        1859  GGATCC               G^GATCC
             2775  GGATCC              
BbvCI         749  CCTCAGC              CCTCAGC(-5/-2)
             4244  GCTGAGG             
BclI         1336  TGATCA               T^GATCA
             2760  TGATCA              
BsaBI        1466  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3532  GATATAAATC          
BspMI         466  GCAGGT               ACCTGC(4/8)
              709  GCAGGT              
EcoT14I       959  CCAAGG               C^C[AT][AT]GG
             1245  CCTTGG              
FbaI         1336  TGATCA               T^GATCA
             2760  TGATCA              
MaeII        1554  ACGT                 A^CGT
             2260  ACGT                
MamI         1466  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3532  GATATAAATC          
MspA1I       3267  CCGCTG               C[AC]G^C[GT]G
             4315  CAGCGG              
PflMI         726  CCATCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4269  CCAGGAGGTGG         
PleI         2716  GACTC                GAGTC(4/5)
             1988  GAGTC               
PstI          464  CTGCAG               CTGCA^G
             1792  CTGCAG              
SapI         1512  GAAGAGC              GCTCTTC(1/4)
             3088  GCTCTTC             
StyI          959  CCAAGG               C^C[AT][AT]GG
             1245  CCTTGG              
SwaI         1995  ATTTAAAT             ATTT^AAAT
             2992  ATTTAAAT            
Van91I        726  CCATCAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4269  CCAGGAGGTGG         
XcmI         1653  CCAGAGGTGGAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3057  CCAGGATATTGATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AvaII       BanI        BbsI        BbvI        
BfaI        BpmI        BpuAI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmI        BsrDI       
BsrI        BssKI       BstF5I      BstNI       BstXI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraI        EarI        EcoRII      EcoT22I     Fnu4HI      
FokI        HaeIII      HincII      HindII      HindIII     
HinfI       HpaI        HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MfeI        
MflI        MnlI        MseI        MslI        MunI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NsiI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        SspI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII