################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII Acc65I AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbrPI BciVI BglII BlnI BlpI Bpu1102I BsiWI BsmI BssHII Bst1107I BstAPI BstBI BstXI BstYI BstZ17I CelII DraIII Eam1105I Eco47III EcoRI EcoRV FseI FspI HpaI KpnI MflI MluI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII SalI SbfI SexAI SfiI SfuI SgrAI SphI Sse8387I StuI XhoI XhoII ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 896 GTCTAC GT^[AC][GT]AC Alw44I 4579 GTGCAC G^TGCAC AlwNI 3383 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 4579 GTGCAC G^TGCAC AspI 156 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1305 TGGCCA TGG^CCA BbsI 2604 GAAGAC GAAGAC(2/6) BbvCI 2165 CCTCAGC CCTCAGC(-5/-2) BglI 554 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 2604 GAAGAC GAAGAC(2/6) BsaBI 4552 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 565 GAGACC GGTCTC(1/5) BsiEI 557 CGGCCG CG[GA][CT]^CG BsmBI 2720 GAGACG CGTCTC(1/5) BspHI 1473 TCATGA T^CATGA BspLU11I 3082 ACATGT A^CATGT BspMI 2421 ACCTGC ACCTGC(4/8) BstEII 1548 GGTCACC G^GT[ACGT]ACC BstPI 1548 GGTCACC G^GT[ACGT]ACC CpoI 31 CGGACCG CG^G[AT]CCG DrdI 1540 GACATTATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 557 CGGCCG C^GGCCG EclXI 557 CGGCCG C^GGCCG Eco52I 557 CGGCCG C^GGCCG EcoO65I 1548 GGTCACC G^GT[ACGT]ACC EcoT22I 3803 ATGCAT ATGCA^T HindIII 2252 AAGCTT A^AGCTT MamI 4552 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3329 CAATTG C^AATTG MluNI 1305 TGGCCA TGG^CCA MscI 1305 TGGCCA TGG^CCA MunI 3329 CAATTG C^AATTG NaeI 559 GCCGGC GCC^GGC NgoMIV 559 GCCGGC G^CCGGC NsiI 3803 ATGCAT ATGCA^T PflFI 156 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4231 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1696 AGGTCCC [GA]G^G[AT]CC[CT] PstI 2135 CTGCAG CTGCA^G RcaI 1473 TCATGA T^CATGA RsrII 31 CGGACCG CG^G[AT]CCG SacI 2352 GAGCTC GAGCT^C SapI 2141 GCTCTTC GCTCTTC(1/4) SnaBI 2071 TACGTA TAC^GTA SwaI 3369 ATTTAAAT ATTT^AAAT Tth111I 156 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4231 CCAACATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3175 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 248 TCCGGA T^CCGGA 1512 TCCGGA AflII 1078 CTTAAG C^TTAAG 3916 CTTAAG AflIII 831 ACACGT A^C[GA][CT]GT 3082 ACATGT BcgI 1941 CGAGATGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 335 GCAACCGCCTCG BclI 1800 TGATCA T^GATCA 3797 TGATCA BfrI 1078 CTTAAG C^TTAAG 3916 CTTAAG BpmI 2324 CTCCAG CTGGAG(16/14) 2015 CTGGAG BsaAI 2071 TACGTA [CT]AC^GT[GA] 3702 CACGTA BseAI 248 TCCGGA T^CCGGA 1512 TCCGGA BseRI 411 CTCCTC GAGGAG(10/8) 1882 GAGGAG Bsp1407I 1038 TGTACA T^GTACA 3085 TGTACA BspDI 1454 ATCGAT AT^CGAT 2066 ATCGAT BspEI 248 TCCGGA T^CCGGA 1512 TCCGGA BsrBI 487 CCGCTC CCGCTC(-3/-3) 631 GAGCGG BsrGI 1038 TGTACA T^GTACA 3085 TGTACA BssSI 1565 CACGAG CACGAG(-5/-1) 2740 CTCGTG ClaI 1454 ATCGAT AT^CGAT 2066 ATCGAT EcoNI 946 CCTAATATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1957 CCTCTTGTAGG FbaI 1800 TGATCA T^GATCA 3797 TGATCA HincII 2475 GTCAAC GT[CT]^[GA]AC 4189 GTTGAC HindII 2475 GTCAAC GT[CT]^[GA]AC 4189 GTTGAC MroI 248 TCCGGA T^CCGGA 1512 TCCGGA NdeI 2042 CATATG CA^TATG 4235 CATATG NspI 2457 ACATGC [GA]CATG^[CT] 3082 ACATGT PleI 2322 GACTC GAGTC(4/5) 1254 GAGTC ScaI 2314 AGTACT AGT^ACT 3912 AGTACT SmaI 84 CCCGGG CCC^GGG 536 CCCGGG SpeI 2611 ACTAGT A^CTAGT 4135 ACTAGT SspBI 1038 TGTACA T^GTACA 3085 TGTACA XcmI 812 CCAAAGTCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2609 CCACTAGTTCAATGG XmaCI 84 CCCGGG C^CCGGG 536 CCCGGG XmaI 84 CCCGGG C^CCGGG 536 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AocI ApaI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BsaHI BsaJI BsaWI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI