##################################
# NO CUT ENZYMES (   67 enzymes) #
##################################

AatII       Acc65I      AclI        AgeI        AhdI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AspEI       AviII       AvrII       BamHI       BbrPI       
BciVI       BglII       BlnI        BlpI        Bpu1102I    
BsiWI       BsmI        BssHII      Bst1107I    BstAPI      
BstBI       BstXI       BstYI       BstZ17I     CelII       
DraIII      Eam1105I    Eco47III    EcoRI       EcoRV       
FseI        FspI        HpaI        KpnI        MflI        
MluI        NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       SalI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SphI        Sse8387I    StuI        
XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI          896  GTCTAC               GT^[AC][GT]AC
Alw44I       4579  GTGCAC               G^TGCAC
AlwNI        3383  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        4579  GTGCAC               G^TGCAC
AspI          156  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI         1305  TGGCCA               TGG^CCA
BbsI         2604  GAAGAC               GAAGAC(2/6)
BbvCI        2165  CCTCAGC              CCTCAGC(-5/-2)
BglI          554  GCCCGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI        2604  GAAGAC               GAAGAC(2/6)
BsaBI        4552  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          565  GAGACC               GGTCTC(1/5)
BsiEI         557  CGGCCG               CG[GA][CT]^CG
BsmBI        2720  GAGACG               CGTCTC(1/5)
BspHI        1473  TCATGA               T^CATGA
BspLU11I     3082  ACATGT               A^CATGT
BspMI        2421  ACCTGC               ACCTGC(4/8)
BstEII       1548  GGTCACC              G^GT[ACGT]ACC
BstPI        1548  GGTCACC              G^GT[ACGT]ACC
CpoI           31  CGGACCG              CG^G[AT]CCG
DrdI         1540  GACATTATGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          557  CGGCCG               C^GGCCG
EclXI         557  CGGCCG               C^GGCCG
Eco52I        557  CGGCCG               C^GGCCG
EcoO65I      1548  GGTCACC              G^GT[ACGT]ACC
EcoT22I      3803  ATGCAT               ATGCA^T
HindIII      2252  AAGCTT               A^AGCTT
MamI         4552  GATGCAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         3329  CAATTG               C^AATTG
MluNI        1305  TGGCCA               TGG^CCA
MscI         1305  TGGCCA               TGG^CCA
MunI         3329  CAATTG               C^AATTG
NaeI          559  GCCGGC               GCC^GGC
NgoMIV        559  GCCGGC               G^CCGGC
NsiI         3803  ATGCAT               ATGCA^T
PflFI         156  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        4231  CCAACATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1696  AGGTCCC              [GA]G^G[AT]CC[CT]
PstI         2135  CTGCAG               CTGCA^G
RcaI         1473  TCATGA               T^CATGA
RsrII          31  CGGACCG              CG^G[AT]CCG
SacI         2352  GAGCTC               GAGCT^C
SapI         2141  GCTCTTC              GCTCTTC(1/4)
SnaBI        2071  TACGTA               TAC^GTA
SwaI         3369  ATTTAAAT             ATTT^AAAT
Tth111I       156  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       4231  CCAACATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3175  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccIII        248  TCCGGA               T^CCGGA
             1512  TCCGGA              
AflII        1078  CTTAAG               C^TTAAG
             3916  CTTAAG              
AflIII        831  ACACGT               A^C[GA][CT]GT
             3082  ACATGT              
BcgI         1941  CGAGATGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              335  GCAACCGCCTCG        
BclI         1800  TGATCA               T^GATCA
             3797  TGATCA              
BfrI         1078  CTTAAG               C^TTAAG
             3916  CTTAAG              
BpmI         2324  CTCCAG               CTGGAG(16/14)
             2015  CTGGAG              
BsaAI        2071  TACGTA               [CT]AC^GT[GA]
             3702  CACGTA              
BseAI         248  TCCGGA               T^CCGGA
             1512  TCCGGA              
BseRI         411  CTCCTC               GAGGAG(10/8)
             1882  GAGGAG              
Bsp1407I     1038  TGTACA               T^GTACA
             3085  TGTACA              
BspDI        1454  ATCGAT               AT^CGAT
             2066  ATCGAT              
BspEI         248  TCCGGA               T^CCGGA
             1512  TCCGGA              
BsrBI         487  CCGCTC               CCGCTC(-3/-3)
              631  GAGCGG              
BsrGI        1038  TGTACA               T^GTACA
             3085  TGTACA              
BssSI        1565  CACGAG               CACGAG(-5/-1)
             2740  CTCGTG              
ClaI         1454  ATCGAT               AT^CGAT
             2066  ATCGAT              
EcoNI         946  CCTAATATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1957  CCTCTTGTAGG         
FbaI         1800  TGATCA               T^GATCA
             3797  TGATCA              
HincII       2475  GTCAAC               GT[CT]^[GA]AC
             4189  GTTGAC              
HindII       2475  GTCAAC               GT[CT]^[GA]AC
             4189  GTTGAC              
MroI          248  TCCGGA               T^CCGGA
             1512  TCCGGA              
NdeI         2042  CATATG               CA^TATG
             4235  CATATG              
NspI         2457  ACATGC               [GA]CATG^[CT]
             3082  ACATGT              
PleI         2322  GACTC                GAGTC(4/5)
             1254  GAGTC               
ScaI         2314  AGTACT               AGT^ACT
             3912  AGTACT              
SmaI           84  CCCGGG               CCC^GGG
              536  CCCGGG              
SpeI         2611  ACTAGT               A^CTAGT
             4135  ACTAGT              
SspBI        1038  TGTACA               T^GTACA
             3085  TGTACA              
XcmI          812  CCAAAGTCAAATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2609  CCACTAGTTCAATGG     
XmaCI          84  CCCGGG               C^CCGGG
              536  CCCGGG              
XmaI           84  CCCGGG               C^CCGGG
              536  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AocI        ApaI        ApoI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbeI        BbvI        BcnI        BfaI        
BmyI        BsaHI       BsaJI       BsaWI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstUI       Bsu36I      
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NcoI        NdeII       NlaIII      
NlaIV       RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SmlI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XmnI