##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AgeI        AhdI        AocI        Aor51HI     AscI        
Asp718I     AspEI       AspI        BbeI        BbrPI       
BbvCI       BfrI        BglI        BsaAI       BsaHI       
BsgI        BsiWI       BspDI       BssHII      BssSI       
BstEII      BstPI       Bsu36I      ClaI        CpoI        
DraIII      Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoRV       EcoT22I     Hin1I       KasI        KpnI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RsrII       SbfI        ScaI        SexAI       SfiI        
SfoI        SnaBI       SphI        Sse8387I    SspI        
SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII       2175  TCCGGA               T^CCGGA
Alw44I       1351  GTGCAC               G^TGCAC
AlwNI        3488  CAGTGACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2024  GGGCCC               GGGCC^C
ApaLI        1351  GTGCAC               G^TGCAC
AviII        1045  TGCGCA               TGC^GCA
AvrII        2011  CCTAGG               C^CTAGG
BalI         3566  TGGCCA               TGG^CCA
BamHI        3509  GGATCC               G^GATCC
BbsI         3534  GAAGAC               GAAGAC(2/6)
BcgI         3621  GCAGCACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2849  GGATAC               GTATCC(6/5)
BclI         1906  TGATCA               T^GATCA
BglII        1076  AGATCT               A^GATCT
BlnI         2011  CCTAGG               C^CTAGG
BpuAI        3534  GAAGAC               GAAGAC(2/6)
BsaBI        1884  GATTTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         3418  GAGACC               GGTCTC(1/5)
BseAI        2175  TCCGGA               T^CCGGA
BspEI        2175  TCCGGA               T^CCGGA
BspLU11I     3238  ACATGT               A^CATGT
BsrDI        1266  GCAATG               GCAATG(2/0)
Bst1107I     2398  GTATAC               GTA^TAC
BstAPI        109  GCAAGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1140  TTCGAA               TT^CGAA
BstZ17I      2398  GTATAC               GTA^TAC
DraI         1823  TTTAAA               TTT^AAA
DrdI         3049  GACCTCAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1095  CGGCCG               C^GGCCG
EclXI        1095  CGGCCG               C^GGCCG
Eco52I       1095  CGGCCG               C^GGCCG
FbaI         1906  TGATCA               T^GATCA
FseI         1092  GGCCGGCC             GGCCGG^CC
FspI         1045  TGCGCA               TGC^GCA
HindIII      1306  AAGCTT               A^AGCTT
HpaI         3796  GTTAAC               GTT^AAC
MamI         1884  GATTTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1041  CAATTG               C^AATTG
MluI          296  ACGCGT               A^CGCGT
MluNI        3566  TGGCCA               TGG^CCA
MroI         2175  TCCGGA               T^CCGGA
MscI         3566  TGGCCA               TGG^CCA
MunI         1041  CAATTG               C^AATTG
NaeI         1093  GCCGGC               GCC^GGC
NcoI         3704  CCATGG               C^CATGG
NgoMIV       1093  GCCGGC               G^CCGGC
NspV         1140  TTCGAA               TT^CGAA
PflMI         351  CCAACTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI         3876  GACTC                GAGTC(4/5)
SfuI         1140  TTCGAA               TT^CGAA
SmaI          654  CCCGGG               CCC^GGG
SpeI         2769  ACTAGT               A^CTAGT
StuI         2668  AGGCCT               AGG^CCT
Van91I        351  CCAACTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2841  TCTAGA               T^CTAGA
XmaCI         654  CCCGGG               C^CCGGG
XmaI          654  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AflIII        296  ACGCGT               A^C[GA][CT]GT
             3238  ACATGT              
AseI         1656  ATTAAT               AT^TAAT
             3670  ATTAAT              
AsnI         1656  ATTAAT               AT^TAAT
             3670  ATTAAT              
BlpI         2898  GCTCAGC              GC^T[ACGT]AGC
             3076  GCTGAGC             
BpmI         3401  CTCCAG               CTGGAG(16/14)
              741  CTGGAG              
Bpu1102I     2898  GCTCAGC              GC^T[ACGT]AGC
             3076  GCTGAGC             
BsiEI         719  CGACCG               CG[GA][CT]^CG
             1095  CGGCCG              
BsmI         2745  GAATGC               GAATGC(1/-1)
             1364  GCATTC              
Bsp1407I     3233  TGTACA               T^GTACA
             3867  TGTACA              
BsrBI        2614  CCGCTC               CCGCTC(-3/-3)
             2845  GAGCGG              
BsrGI        3233  TGTACA               T^GTACA
             3867  TGTACA              
BstXI         248  CCAGGGCGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3398  CCACTCCAGTGG        
CelII        2898  GCTCAGC              GC^T[ACGT]AGC
             3076  GCTGAGC             
EcoNI         304  CCTCGCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2163  CCTGCGTGAGG         
EcoRI        1931  GAATTC               G^AATTC
             2474  GAATTC              
HaeII         258  GGCGCT               [GA]GCGC^[CT]
              612  GGCGCT              
PshAI          28  GACTGCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2362  GACAATCGTC          
PshBI        1656  ATTAAT               AT^TAAT
             3670  ATTAAT              
SacI         2419  GAGCTC               GAGCT^C
             2992  GAGCTC              
SalI         3409  GTCGAC               G^TCGAC
             3462  GTCGAC              
SapI          427  GAAGAGC              GCTCTTC(1/4)
             2059  GCTCTTC             
SgrAI         120  CGCCGGTG             C[GA]^CCGG[CT]G
              673  CACCGGCG            
SmlI         2259  CTTGAG               C^T[CT][GA]AG
             2559  CTTGAG              
SspBI        3233  TGTACA               T^GTACA
             3867  TGTACA              
XcmI         3403  CCAGTGGTCGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3569  CCATCACACTCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  108 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BsaJI       BsaWI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspHI       
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PpuMI       PstI        PvuII       RcaI        
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       XmnI