##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI BspDI BspEI BssSI
BstBI Bsu36I CelII ClaI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV FseI FspI HpaI
KasI KpnI KspI MamI MluI
MroI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I SacI SacII SalI SbfI
SfoI SfuI SgrAI SpeI Sse8387I
SspI StuI SwaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 1274 GACGCC G[GA]^CG[CT]C
AflII 308 CTTAAG C^TTAAG
ApaI 624 GGGCCC GGGCC^C
BamHI 3812 GGATCC G^GATCC
BanI 3331 GGTGCC G^G[CT][GA]CC
BclI 177 TGATCA T^GATCA
BfrI 308 CTTAAG C^TTAAG
BglII 927 AGATCT A^GATCT
BsaHI 1274 GACGCC G[GA]^CG[CT]C
BsaWI 1387 ACCGGA [AT]^CCGG[AT]
BspLU11I 3065 ACATGT A^CATGT
BsrBI 1214 GAGCGG CCGCTC(-3/-3)
BsrFI 1392 ACCGGC [GA]^CCGG[CT]
BssHII 1051 GCGCGC G^CGCGC
Bst1107I 3731 GTATAC GTA^TAC
BstAPI 2219 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3731 GTATAC GTA^TAC
Cfr10I 1392 ACCGGC [GA]^CCGG[CT]
CpoI 1389 CGGACCG CG^G[AT]CCG
DrdI 2940 GACAGAGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1012 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 177 TGATCA T^GATCA
HgaI 1274 GACGC GACGC(5/10)
Hin1I 1274 GACGCC G[GA]^CG[CT]C
NcoI 1844 CCATGG C^CATGG
NdeI 3889 CATATG CA^TATG
PaeR7I 803 CTCGAG C^TCGAG
PflMI 1222 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 407 AGGACCT [GA]G^G[AT]CC[CT]
RsrII 1389 CGGACCG CG^G[AT]CCG
SnaBI 1667 TACGTA TAC^GTA
SphI 1146 GCATGC GCATG^C
Van91I 1222 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3337 TCTAGA T^CTAGA
XhoI 803 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 2629 GTCTAC GT^[AC][GT]AC
3731 GTATAC
AflIII 61 ACACGT A^C[GA][CT]GT
3065 ACATGT
Alw44I 369 GTGCAC G^TGCAC
1790 GTGCAC
ApaLI 369 GTGCAC G^TGCAC
1790 GTGCAC
Asp700I 1972 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3211 GAATAATTTC
BbsI 1435 GAAGAC GAAGAC(2/6)
2584 GAAGAC
BpuAI 1435 GAAGAC GAAGAC(2/6)
2584 GAAGAC
BsaAI 62 CACGTA [CT]AC^GT[GA]
1667 TACGTA
BspHI 1167 TCATGA T^CATGA
3946 TCATGA
BsrDI 521 CATTGC GCAATG(2/0)
2317 GCAATG
BstEII 892 GGTCACC G^GT[ACGT]ACC
2024 GGTGACC
BstPI 892 GGTCACC G^GT[ACGT]ACC
2024 GGTGACC
BstXI 990 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1401 CCAGCAATTTGG
DsaI 1080 CCGTGG C^C[GA][CT]GG
1844 CCATGG
EcoO65I 892 GGTCACC G^GT[ACGT]ACC
2024 GGTGACC
EcoT22I 1641 ATGCAT ATGCA^T
2353 ATGCAT
HincII 2650 GTTGAC GT[CT]^[GA]AC
3984 GTCAAC
HindII 2650 GTTGAC GT[CT]^[GA]AC
3984 GTCAAC
NsiI 1641 ATGCAT ATGCA^T
2353 ATGCAT
PstI 767 CTGCAG CTGCA^G
1312 CTGCAG
PvuI 563 CGATCG CGAT^CG
3354 CGATCG
RcaI 1167 TCATGA T^CATGA
3946 TCATGA
ScaI 555 AGTACT AGT^ACT
3917 AGTACT
SexAI 759 ACCTGGT A^CC[AT]GGT
1011 ACCTGGT
SfiI 532 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
608 GGCCAGGACGGCC
SmaI 698 CCCGGG CCC^GGG
744 CCCGGG
XmaCI 698 CCCGGG C^CCGGG
744 CCCGGG
XmaI 698 CCCGGG C^CCGGG
744 CCCGGG
XmnI 1972 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3211 GAATAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MunI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XcmI
XhoII