##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflIII      AgeI        AhdI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        BbeI        
BcgI        BglI        BsaBI       BsaHI       BseAI       
BsiWI       BsmBI       BspDI       BspEI       BspHI       
BspLU11I    BstBI       ClaI        CpoI        DrdI        
Eam1105I    HgaI        Hin1I       HpaI        KasI        
KpnI        MamI        MfeI        MluI        MroI        
MunI        NarI        NdeI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SalI        
SapI        SbfI        SexAI       SfiI        SfoI        
SfuI        SnaBI       SpeI        Sse8387I    SspI        
Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AocI          436  CCTAAGG              CC^T[ACGT]AGG
Asp700I      1578  GAATGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        3229  TGCGCA               TGC^GCA
AvrII         677  CCTAGG               C^CTAGG
BalI         2533  TGGCCA               TGG^CCA
BamHI        3071  GGATCC               G^GATCC
BanI         3628  GGTGCC               G^G[CT][GA]CC
BbrPI         111  CACGTG               CAC^GTG
BbvCI        1907  CCTCAGC              CCTCAGC(-5/-2)
BclI         4182  TGATCA               T^GATCA
BglII        1241  AGATCT               A^GATCT
BlnI          677  CCTAGG               C^CTAGG
BlpI          381  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      381  GCTGAGC              GC^T[ACGT]AGC
BsaI          775  GGTCTC               GGTCTC(1/5)
Bst1107I     3148  GTATAC               GTA^TAC
BstAPI       1833  GCAGCCATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        3174  CCACTACCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      3148  GTATAC               GTA^TAC
Bsu36I        436  CCTAAGG              CC^T[ACGT]AGG
CelII         381  GCTGAGC              GC^T[ACGT]AGC
DraIII        970  CACTCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        436  CCTAAGG              CC^T[ACGT]AGG
EcoNI         177  CCTCCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        2458  GAATTC               G^AATTC
EcoT22I      3358  ATGCAT               ATGCA^T
FbaI         4182  TGATCA               T^GATCA
FseI          341  GGCCGGCC             GGCCGG^CC
FspI         3229  TGCGCA               TGC^GCA
MluNI        2533  TGGCCA               TGG^CCA
MscI         2533  TGGCCA               TGG^CCA
NcoI         2845  CCATGG               C^CATGG
NheI          212  GCTAGC               G^CTAGC
NsiI         3358  ATGCAT               ATGCA^T
PmaCI         111  CACGTG               CAC^GTG
PmlI          111  CACGTG               CAC^GTG
PvuII          80  CAGCTG               CAG^CTG
ScaI         3916  AGTACT               AGT^ACT
SgrAI         420  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          373  CCCGGG               CCC^GGG
StuI         1509  AGGCCT               AGG^CCT
SwaI         3474  ATTTAAAT             ATTT^AAAT
XmaCI         373  CCCGGG               C^CCGGG
XmaI          373  CCCGGG               C^CCGGG
XmnI         1578  GAATGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccI         3148  GTATAC               GT^[AC][GT]AC
             3615  GTAGAC              
AflII          23  CTTAAG               C^TTAAG
             3980  CTTAAG              
Alw44I        967  GTGCAC               G^TGCAC
             3807  GTGCAC              
Aor51HI         4  AGCGCT               AGC^GCT
              324  AGCGCT              
ApaI          493  GGGCCC               GGGCC^C
             2552  GGGCCC              
ApaLI         967  GTGCAC               G^TGCAC
             3807  GTGCAC              
AvaII         587  GGTCC                G^G[AT]CC
              922  GGACC               
BfrI           23  CTTAAG               C^TTAAG
             3980  CTTAAG              
BsgI         3043  CTGCAC               GTGCAG(16/14)
             1997  GTGCAG              
BsmI         1578  GAATGC               GAATGC(1/-1)
             4078  GCATTC              
Bsp1407I     4020  TGTACA               T^GTACA
             4056  TGTACA              
BsrBI         616  CCGCTC               CCGCTC(-3/-3)
              995  CCGCTC              
BsrDI        1838  CATTGC               GCAATG(2/0)
             1461  GCAATG              
BsrGI        4020  TGTACA               T^GTACA
             4056  TGTACA              
DraII         492  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             2552  GGGCCCC             
EagI          340  CGGCCG               C^GGCCG
              498  CGGCCG              
EclXI         340  CGGCCG               C^GGCCG
              498  CGGCCG              
Eco47III        4  AGCGCT               AGC^GCT
              324  AGCGCT              
Eco52I        340  CGGCCG               C^GGCCG
              498  CGGCCG              
EcoO109I      492  GGGGCCC              [GA]G^G[ACGT]CC[CT]
             2552  GGGCCCC             
EcoRV        2043  GATATC               GAT^ATC
             2487  GATATC              
HaeII           4  AGCGCT               [GA]GCGC^[CT]
              324  AGCGCT              
HincII       2633  GTCAAC               GT[CT]^[GA]AC
             3445  GTCAAC              
HindII       2633  GTCAAC               GT[CT]^[GA]AC
             3445  GTCAAC              
HindIII       863  AAGCTT               A^AGCTT
             3350  AAGCTT              
KspI          583  CCGCGG               CCGC^GG
              918  CCGCGG              
MslI         2048  CACCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4079  CATTCATGTG          
SacI          638  GAGCTC               GAGCT^C
             3282  GAGCTC              
SacII         583  CCGCGG               CCGC^GG
              918  CCGCGG              
SphI         1498  GCATGC               GCATG^C
             3226  GCATGC              
SspBI        4020  TGTACA               T^GTACA
             4056  TGTACA              
TaqI         2041  TCGA                 T^CGA
             2687  TCGA                
TthHB8I      2041  TCGA                 T^CGA
             2687  TCGA                
XcmI         2740  CCACGATCAACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4075  CCAGCATTCATGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
BanII       BbsI        BbvI        BciVI       BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaAI       
BsaJI       BsaWI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrFI       BsrI        BssHII      
BssKI       BssSI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DsaI        EaeI        EarI        EcoO65I     EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PleI        PstI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       XhoII