##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII BamHI
BbrPI BcgI BciVI BsaAI BsaBI
BsaI BseAI BsiWI BsmBI BspDI
BspEI BspMI BssHII BssSI BstBI
BstEII BstPI BstXI Bsu36I ClaI
CpoI DraII Eam1105I Eco47III Eco81I
EcoO109I EcoO65I EcoT22I FseI FspI
HgaI KpnI MamI MluI MroI
NdeI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SalI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1166 GACGTC GACGT^C
AflII 3299 CTTAAG C^TTAAG
AflIII 945 ACATGT A^C[GA][CT]GT
Alw44I 733 GTGCAC G^TGCAC
ApaLI 733 GTGCAC G^TGCAC
AvrII 3034 CCTAGG C^CTAGG
BalI 4511 TGGCCA TGG^CCA
BclI 1596 TGATCA T^GATCA
BfrI 3299 CTTAAG C^TTAAG
BglII 1532 AGATCT A^GATCT
BlnI 3034 CCTAGG C^CTAGG
BlpI 1801 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1801 GCTCAGC GC^T[ACGT]AGC
BsaWI 776 ACCGGA [AT]^CCGG[AT]
BsmI 3876 GAATGC GAATGC(1/-1)
Bsp1407I 2225 TGTACA T^GTACA
BspHI 359 TCATGA T^CATGA
BspLU11I 945 ACATGT A^CATGT
BsrBI 245 GAGCGG CCGCTC(-3/-3)
BsrGI 2225 TGTACA T^GTACA
Bst1107I 4655 GTATAC GTA^TAC
BstAPI 1745 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 4655 GTATAC GTA^TAC
CelII 1801 GCTCAGC GC^T[ACGT]AGC
DraIII 4395 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 694 GACAACAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1431 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 496 GAATTC G^AATTC
FbaI 1596 TGATCA T^GATCA
MluNI 4511 TGGCCA TGG^CCA
MscI 4511 TGGCCA TGG^CCA
NcoI 1709 CCATGG C^CATGG
NheI 1344 GCTAGC G^CTAGC
PaeR7I 2056 CTCGAG C^TCGAG
PflMI 1642 CCAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 359 TCATGA T^CATGA
SapI 427 GCTCTTC GCTCTTC(1/4)
SgrAI 62 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1702 CCCGGG CCC^GGG
SspBI 2225 TGTACA T^GTACA
Van91I 1642 CCAATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2056 CTCGAG C^TCGAG
XmaCI 1702 CCCGGG C^CCGGG
XmaI 1702 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 2226 GTAC GT^AC
3388 GTAC
AseI 3657 ATTAAT AT^TAAT
4646 ATTAAT
AsnI 3657 ATTAAT AT^TAAT
4646 ATTAAT
BglI 241 GCCGGAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
331 GCCGCGGCGGC
BsgI 550 CTGCAC GTGCAG(16/14)
1408 CTGCAC
BstYI 366 AGATCC [GA]^GATC[CT]
1532 AGATCT
EcoRV 1894 GATATC GAT^ATC
2088 GATATC
HindIII 2782 AAGCTT A^AGCTT
3092 AAGCTT
HpaI 1284 GTTAAC GTT^AAC
3763 GTTAAC
KspI 282 CCGCGG CCGC^GG
332 CCGCGG
MfeI 1643 CAATTG C^AATTG
3333 CAATTG
MflI 366 AGATCC [GA]^GATC[CT]
1532 AGATCT
MunI 1643 CAATTG C^AATTG
3333 CAATTG
PshAI 775 GACCGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACCTTTGTC
PshBI 3657 ATTAAT AT^TAAT
4646 ATTAAT
PstI 100 CTGCAG CTGCA^G
986 CTGCAG
RsaI 2226 GTAC GT^AC
3388 GTAC
SacI 1799 GAGCTC GAGCT^C
4455 GAGCTC
SacII 282 CCGCGG CCGC^GG
332 CCGCGG
SspI 1315 AATATT AAT^ATT
4825 AATATT
XhoII 366 AGATCC [GA]^GATC[CT]
1532 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DsaI EaeI
EagI EarI EclXI Eco52I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmnI