##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AviII AvrII BbeI BbvCI BclI
BlnI BpmI BsaHI BsaI BsaWI
BseAI BsgI BsiWI BsmI Bsp1407I
BspEI BspHI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI Bsu36I
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FbaI FseI FspI HgaI Hin1I
KasI KpnI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI Psp1406I PstI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI Sse8387I SspBI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 4319 GTATAC GT^[AC][GT]AC
AflII 3806 CTTAAG C^TTAAG
AspI 2834 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3190 CTCGGG C^[CT]CG[GA]G
BalI 3265 TGGCCA TGG^CCA
BamHI 2707 GGATCC G^GATCC
BbrPI 708 CACGTG CAC^GTG
BcgI 2287 GCACTTGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3806 CTTAAG C^TTAAG
BglII 578 AGATCT A^GATCT
BsiEI 363 CGACCG CG[GA][CT]^CG
BsoBI 3190 CTCGGG C^[CT]CG[GA]G
BspDI 2295 ATCGAT AT^CGAT
BspMI 701 ACCTGC ACCTGC(4/8)
BsrBI 281 GAGCGG CCGCTC(-3/-3)
BsrDI 354 GCAATG GCAATG(2/0)
BsrFI 350 ACCGGC [GA]^CCGG[CT]
Bst1107I 4319 GTATAC GTA^TAC
BstXI 389 CCACTTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4319 GTATAC GTA^TAC
Cfr10I 350 ACCGGC [GA]^CCGG[CT]
ClaI 2295 ATCGAT AT^CGAT
EaeI 3265 TGGCCA [CT]^GGCC[GA]
EcoRV 2242 GATATC GAT^ATC
HaeII 1600 GGCGCT [GA]GCGC^[CT]
HpaI 3969 GTTAAC GTT^AAC
KspI 20 CCGCGG CCGC^GG
MfeI 2939 CAATTG C^AATTG
MluNI 3265 TGGCCA TGG^CCA
MscI 3265 TGGCCA TGG^CCA
MunI 2939 CAATTG C^AATTG
NcoI 168 CCATGG C^CATGG
PflFI 2834 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3092 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 708 CACGTG CAC^GTG
PmlI 708 CACGTG CAC^GTG
SacI 403 GAGCTC GAGCT^C
SacII 20 CCGCGG CCGC^GG
SnaBI 3906 TACGTA TAC^GTA
SpeI 685 ACTAGT A^CTAGT
SphI 3823 GCATGC GCATG^C
StuI 120 AGGCCT AGG^CCT
Tth111I 2834 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3092 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2753 CCAAGGGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 403 GAGCTC G[AT]GC[AT]^C
757 GTGCTC
BanI 370 GGCACC G^G[CT][GA]CC
2490 GGCACC
BanII 42 GAGCCC G[GA]GC[CT]^C
403 GAGCTC
BciVI 309 GGATAC GTATCC(6/5)
501 GGATAC
BcnI 110 CCGGG CC^[GC]GG
374 CCGGG
BglI 122 GCCTCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3060 GCCTTTTAGGC
BlpI 1000 GCTCAGC GC^T[ACGT]AGC
1519 GCTCAGC
Bpu1102I 1000 GCTCAGC GC^T[ACGT]AGC
1519 GCTCAGC
BsaBI 1579 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1909 GATAATTATC
BsiHKAI 403 GAGCTC G[AT]GC[AT]^C
757 GTGCTC
CelII 1000 GCTCAGC GC^T[ACGT]AGC
1519 GCTCAGC
DsaI 20 CCGCGG C^C[GA][CT]GG
168 CCATGG
EcoNI 912 CCTAAATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1588 CCTGAGCTAGG
FauI 28 GCGGG CCCGC(4/6)
82 GCGGG
HincII 3624 GTCAAC GT[CT]^[GA]AC
3969 GTTAAC
HindII 3624 GTCAAC GT[CT]^[GA]AC
3969 GTTAAC
HindIII 842 AAGCTT A^AGCTT
2930 AAGCTT
MamI 1579 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1909 GATAATTATC
MslI 390 CACTTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
880 CATTTGTATG
NciI 110 CCGGG CC^[GC]GG
374 CCGGG
PpuMI 635 AGGACCT [GA]G^G[AT]CC[CT]
2866 GGGACCC
SspI 2854 AATATT AAT^ATT
3979 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AvaII BbsI BbvI
BfaI BmyI BpuAI BsaAI BsaJI
BseRI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspLU11I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI