##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AhdI ApoI AscI AseI AsnI
AspEI AspI BcgI BclI BfrI
BsaBI BsiWI BsmBI BsmI Bsp1407I
BspDI BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI Eam1105I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HpaI MamI MfeI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PflFI PmeI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfuI SmaI SnaBI SpeI SphI
SspBI SspI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 68 GGTACC G^GTACC
AgeI 1296 ACCGGT A^CCGGT
AlwNI 906 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2269 AGCGCT AGC^GCT
Asp718I 68 GGTACC G^GTACC
AviII 1401 TGCGCA TGC^GCA
BalI 3903 TGGCCA TGG^CCA
BamHI 1288 GGATCC G^GATCC
BciVI 3578 GTATCC GTATCC(6/5)
BglII 955 AGATCT A^GATCT
BlpI 1002 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1002 GCTGAGC GC^T[ACGT]AGC
BsiEI 1891 CGGCCG CG[GA][CT]^CG
BspHI 3564 TCATGA T^CATGA
BspLU11I 1450 ACATGT A^CATGT
CelII 1002 GCTGAGC GC^T[ACGT]AGC
EagI 1891 CGGCCG C^GGCCG
EclXI 1891 CGGCCG C^GGCCG
Eco47III 2269 AGCGCT AGC^GCT
Eco52I 1891 CGGCCG C^GGCCG
FspI 1401 TGCGCA TGC^GCA
HincII 3647 GTTGAC GT[CT]^[GA]AC
HindII 3647 GTTGAC GT[CT]^[GA]AC
HindIII 1334 AAGCTT A^AGCTT
KpnI 68 GGTACC GGTAC^C
KspI 2326 CCGCGG CCGC^GG
MluI 2113 ACGCGT A^CGCGT
MluNI 3903 TGGCCA TGG^CCA
MscI 3903 TGGCCA TGG^CCA
NcoI 1669 CCATGG C^CATGG
NheI 2759 GCTAGC G^CTAGC
PaeR7I 1806 CTCGAG C^TCGAG
PflMI 905 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1296 ACCGGT A^CCGGT
PshAI 2400 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3564 TCATGA T^CATGA
SacI 1269 GAGCTC GAGCT^C
SacII 2326 CCGCGG CCGC^GG
SapI 420 GAAGAGC GCTCTTC(1/4)
SbfI 824 CCTGCAGG CCTGCA^GG
SfiI 2419 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 824 CCTGCAGG CCTGCA^GG
TaqI 1807 TCGA T^CGA
TthHB8I 1807 TCGA T^CGA
Van91I 905 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 970 CCACTGACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1806 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccIII 133 TCCGGA T^CCGGA
2598 TCCGGA
AcyI 2340 GGCGCC G[GA]^CG[CT]C
2410 GGCGCC
BbeI 2340 GGCGCC GGCGC^C
2410 GGCGCC
BbrPI 566 CACGTG CAC^GTG
1794 CACGTG
BbsI 1415 GAAGAC GAAGAC(2/6)
3779 GAAGAC
BpuAI 1415 GAAGAC GAAGAC(2/6)
3779 GAAGAC
BsaAI 566 CACGTG [CT]AC^GT[GA]
1794 CACGTG
BsaHI 2340 GGCGCC G[GA]^CG[CT]C
2410 GGCGCC
BseAI 133 TCCGGA T^CCGGA
2598 TCCGGA
BspEI 133 TCCGGA T^CCGGA
2598 TCCGGA
BssHII 1937 GCGCGC G^CGCGC
2305 GCGCGC
BstXI 2948 CCAGGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3452 CCAGGGGTCTGG
DraI 264 TTTAAA TTT^AAA
4116 TTTAAA
Hin1I 2340 GGCGCC G[GA]^CG[CT]C
2410 GGCGCC
KasI 2340 GGCGCC G^GCGCC
2410 GGCGCC
MroI 133 TCCGGA T^CCGGA
2598 TCCGGA
NarI 2340 GGCGCC GG^CGCC
2410 GGCGCC
NspI 622 GCATGT [GA]CATG^[CT]
1450 ACATGT
PmaCI 566 CACGTG CAC^GTG
1794 CACGTG
PmlI 566 CACGTG CAC^GTG
1794 CACGTG
SfoI 2340 GGCGCC GGC^GCC
2410 GGCGCC
TfiI 2602 GATTC G^A[AT]TC
2630 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
Alw44I AlwI AocI ApaI ApaLI
Asp700I AspHI AvaI AvaII AvrII
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlnI BmyI BpmI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SgrAI SmlI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII XmnI