##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AvrII BbeI
BcgI BglI BlnI BsaHI BseAI
BsiEI BsiWI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstZ17I
Cfr10I ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoNI EcoT22I FseI
Hin1I KasI KpnI KspI MluI
MroI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 4229 GTAGAC GT^[AC][GT]AC
AccII 2827 CGCG CG^CG
AflII 1667 CTTAAG C^TTAAG
AocI 503 CCTCAGG CC^T[ACGT]AGG
AseI 1118 ATTAAT AT^TAAT
AsnI 1118 ATTAAT AT^TAAT
AvaI 7 CTCGAG C^[CT]CG[GA]G
AviII 3865 TGCGCA TGC^GCA
BamHI 1889 GGATCC G^GATCC
BbsI 290 GAAGAC GAAGAC(2/6)
BbvCI 4238 GCTGAGG CCTCAGC(-5/-2)
BciVI 1638 GTATCC GTATCC(6/5)
BcnI 2740 CCGGG CC^[GC]GG
BfrI 1667 CTTAAG C^TTAAG
BpuAI 290 GAAGAC GAAGAC(2/6)
BsaBI 4135 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2610 ACCGGA [AT]^CCGG[AT]
BsmBI 183 GAGACG CGTCTC(1/5)
BsoBI 7 CTCGAG C^[CT]CG[GA]G
BspHI 1293 TCATGA T^CATGA
BspLU11I 4594 ACATGT A^CATGT
BstAPI 1201 GCATTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1578 TTCGAA TT^CGAA
BstUI 2827 CGCG CG^CG
BstXI 3984 CCAGCTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 503 CCTCAGG CC^T[ACGT]AGG
DraI 3668 TTTAAA TTT^AAA
DrdI 4668 GACTATAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2812 CCACGG C^C[GA][CT]GG
Eco81I 503 CCTCAGG CC^T[ACGT]AGG
EcoRV 1409 GATATC GAT^ATC
FspI 3865 TGCGCA TGC^GCA
HgaI 415 GACGC GACGC(5/10)
HpaI 3656 GTTAAC GTT^AAC
MamI 4135 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2596 CAATTG C^AATTG
MunI 2596 CAATTG C^AATTG
MvnI 2827 CGCG CG^CG
NciI 2740 CCGGG CC^[GC]GG
NdeI 2340 CATATG CA^TATG
NspV 1578 TTCGAA TT^CGAA
PaeR7I 7 CTCGAG C^TCGAG
PpuMI 1985 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1118 ATTAAT AT^TAAT
RcaI 1293 TCATGA T^CATGA
ScaI 4299 AGTACT AGT^ACT
SfuI 1578 TTCGAA TT^CGAA
StuI 3045 AGGCCT AGG^CCT
XcmI 1146 CCACTTCCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 7 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AflIII 3970 ACACGT A^C[GA][CT]GT
4594 ACATGT
Aor51HI 11 AGCGCT AGC^GCT
334 AGCGCT
AspI 3950 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3976 GACCGAGTC
BanI 1850 GGCACC G^G[CT][GA]CC
3012 GGCACC
BbrPI 3908 CACGTG CAC^GTG
3971 CACGTG
BfaI 279 CTAG C^TAG
1184 CTAG
BglII 354 AGATCT A^GATCT
1963 AGATCT
BlpI 3493 GCTGAGC GC^T[ACGT]AGC
3901 GCTCAGC
Bpu1102I 3493 GCTGAGC GC^T[ACGT]AGC
3901 GCTCAGC
BsaAI 3908 CACGTG [CT]AC^GT[GA]
3971 CACGTG
Bsp1407I 4401 TGTACA T^GTACA
4439 TGTACA
BsrGI 4401 TGTACA T^GTACA
4439 TGTACA
BstEII 655 GGTCACC G^GT[ACGT]ACC
700 GGTGACC
BstPI 655 GGTCACC G^GT[ACGT]ACC
700 GGTGACC
CelII 3493 GCTGAGC GC^T[ACGT]AGC
3901 GCTCAGC
DraIII 1908 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3014 CACCATGTG
Eco47III 11 AGCGCT AGC^GCT
334 AGCGCT
EcoO65I 655 GGTCACC G^GT[ACGT]ACC
700 GGTGACC
FauI 3086 CCCGC CCCGC(4/6)
3092 CCCGC
HaeII 11 AGCGCT [GA]GCGC^[CT]
334 AGCGCT
HapII 2611 CCGG C^CGG
2740 CCGG
HincII 1235 GTCAAC GT[CT]^[GA]AC
3656 GTTAAC
HindII 1235 GTCAAC GT[CT]^[GA]AC
3656 GTTAAC
HindIII 1882 AAGCTT A^AGCTT
3541 AAGCTT
HpaII 2611 CCGG C^CGG
2740 CCGG
MaeI 279 CTAG C^TAG
1184 CTAG
MaeII 3909 ACGT A^CGT
3972 ACGT
MspI 2611 CCGG C^CGG
2740 CCGG
NspI 3103 GCATGT [GA]CATG^[CT]
4594 ACATGT
PflFI 3950 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3976 GACCGAGTC
PmaCI 3908 CACGTG CAC^GTG
3971 CACGTG
PmlI 3908 CACGTG CAC^GTG
3971 CACGTG
SapI 1095 GAAGAGC GCTCTTC(1/4)
3130 GCTCTTC
SspBI 4401 TGTACA T^GTACA
4439 TGTACA
Tth111I 3950 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3976 GACCGAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanII BbvI BclI BmyI
BpmI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI