################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccIII AclI AcsI AflII AhdI AlwNI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BciVI BfrI BglII BlnI BsaBI BseAI BsiWI BsmI BspDI BspEI BspLU11I BsrDI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI MamI MfeI MluI MroI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 950 GGTACC G^GTACC AflIII 1531 ACGTGT A^C[GA][CT]GT AgeI 1975 ACCGGT A^CCGGT AocI 482 CCTGAGG CC^T[ACGT]AGG Asp718I 950 GGTACC G^GTACC BalI 1113 TGGCCA TGG^CCA BamHI 1346 GGATCC G^GATCC BbrPI 1530 CACGTG CAC^GTG BbsI 1437 GTCTTC GAAGAC(2/6) BbvCI 1367 GCTGAGG CCTCAGC(-5/-2) BlpI 1059 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1059 GCTGAGC GC^T[ACGT]AGC BpuAI 1437 GTCTTC GAAGAC(2/6) BsaAI 1530 CACGTG [CT]AC^GT[GA] Bsp1407I 1325 TGTACA T^GTACA BspHI 1453 TCATGA T^CATGA BsrGI 1325 TGTACA T^GTACA BssHII 2364 GCGCGC G^CGCGC Bsu36I 482 CCTGAGG CC^T[ACGT]AGG CelII 1059 GCTGAGC GC^T[ACGT]AGC DrdI 1179 GACTCCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 477 CTCTTC CTCTTC(1/4) Eco81I 482 CCTGAGG CC^T[ACGT]AGG EcoNI 161 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG KpnI 950 GGTACC GGTAC^C Ksp632I 477 CTCTTC CTCTTC(1/4) MluNI 1113 TGGCCA TGG^CCA MscI 1113 TGGCCA TGG^CCA MseI 2899 TTAA T^TAA NcoI 1663 CCATGG C^CATGG NspI 1144 GCATGT [GA]CATG^[CT] PaeR7I 2265 CTCGAG C^TCGAG PflMI 1376 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1975 ACCGGT A^CCGGT PmaCI 1530 CACGTG CAC^GTG PmlI 1530 CACGTG CAC^GTG PshAI 827 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2436 CTGCAG CTGCA^G PvuII 103 CAGCTG CAG^CTG RcaI 1453 TCATGA T^CATGA SacI 1083 GAGCTC GAGCT^C SapI 476 GCTCTTC GCTCTTC(1/4) SspBI 1325 TGTACA T^GTACA StuI 1125 AGGCCT AGG^CCT TfiI 1050 GATTC G^A[AT]TC Tru9I 2899 TTAA T^TAA Tsp509I 2897 AATT ^AATT Van91I 1376 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2265 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccI 1188 GTCTAC GT^[AC][GT]AC 1275 GTCTAC Alw44I 63 GTGCAC G^TGCAC 780 GTGCAC Aor51HI 1295 AGCGCT AGC^GCT 1894 AGCGCT ApaLI 63 GTGCAC G^TGCAC 780 GTGCAC BcgI 119 CGACCTGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1093 CGAACTCCGTGC BsaI 606 GAGACC GGTCTC(1/5) 2029 GAGACC BsrBI 754 GAGCGG CCGCTC(-3/-3) 2104 GAGCGG BssSI 2450 CACGAG CACGAG(-5/-1) 1071 CTCGTG BstEII 762 GGTGACC G^GT[ACGT]ACC 3020 GGTGACC BstPI 762 GGTGACC G^GT[ACGT]ACC 3020 GGTGACC BstXI 1657 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2195 CCAAGACATTGG DraIII 783 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG 2075 CACCGAGTG EagI 1878 CGGCCG C^GGCCG 2014 CGGCCG EclXI 1878 CGGCCG C^GGCCG 2014 CGGCCG Eco47III 1295 AGCGCT AGC^GCT 1894 AGCGCT Eco52I 1878 CGGCCG C^GGCCG 2014 CGGCCG EcoO65I 762 GGTGACC G^GT[ACGT]ACC 3020 GGTGACC MaeII 1531 ACGT A^CGT 1709 ACGT SexAI 1037 ACCAGGT A^CC[AT]GGT 1150 ACCAGGT SfaNI 2084 GATGC GCATC(5/9) 1680 GCATC SfiI 1580 GGCCCTGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1661 GGCCATGGAGGCC SmlI 872 CTTGAG C^T[CT][GA]AG 2265 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AspHI AvaI AvaII BanI BanII BbeI BbvI BclI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI RsaI SacII Sau3AI Sau96I ScrFI SfcI SfoI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I XcmI XhoII XmaCI XmaI