##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AhdI        AlwNI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspI        AviII       
AvrII       BciVI       BfrI        BglII       BlnI        
BsaBI       BseAI       BsiWI       BsmI        BspDI       
BspEI       BspLU11I    BsrDI       Bst1107I    BstAPI      
BstBI       BstZ17I     ClaI        CpoI        DraI        
Eam1105I    EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
MamI        MfeI        MluI        MroI        MslI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SalI        
SbfI        ScaI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        950  GGTACC               G^GTACC
AflIII       1531  ACGTGT               A^C[GA][CT]GT
AgeI         1975  ACCGGT               A^CCGGT
AocI          482  CCTGAGG              CC^T[ACGT]AGG
Asp718I       950  GGTACC               G^GTACC
BalI         1113  TGGCCA               TGG^CCA
BamHI        1346  GGATCC               G^GATCC
BbrPI        1530  CACGTG               CAC^GTG
BbsI         1437  GTCTTC               GAAGAC(2/6)
BbvCI        1367  GCTGAGG              CCTCAGC(-5/-2)
BlpI         1059  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1059  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1437  GTCTTC               GAAGAC(2/6)
BsaAI        1530  CACGTG               [CT]AC^GT[GA]
Bsp1407I     1325  TGTACA               T^GTACA
BspHI        1453  TCATGA               T^CATGA
BsrGI        1325  TGTACA               T^GTACA
BssHII       2364  GCGCGC               G^CGCGC
Bsu36I        482  CCTGAGG              CC^T[ACGT]AGG
CelII        1059  GCTGAGC              GC^T[ACGT]AGC
DrdI         1179  GACTCCCTCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          477  CTCTTC               CTCTTC(1/4)
Eco81I        482  CCTGAGG              CC^T[ACGT]AGG
EcoNI         161  CCTGGCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KpnI          950  GGTACC               GGTAC^C
Ksp632I       477  CTCTTC               CTCTTC(1/4)
MluNI        1113  TGGCCA               TGG^CCA
MscI         1113  TGGCCA               TGG^CCA
MseI         2899  TTAA                 T^TAA
NcoI         1663  CCATGG               C^CATGG
NspI         1144  GCATGT               [GA]CATG^[CT]
PaeR7I       2265  CTCGAG               C^TCGAG
PflMI        1376  CCATTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1975  ACCGGT               A^CCGGT
PmaCI        1530  CACGTG               CAC^GTG
PmlI         1530  CACGTG               CAC^GTG
PshAI         827  GACCCGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         2436  CTGCAG               CTGCA^G
PvuII         103  CAGCTG               CAG^CTG
RcaI         1453  TCATGA               T^CATGA
SacI         1083  GAGCTC               GAGCT^C
SapI          476  GCTCTTC              GCTCTTC(1/4)
SspBI        1325  TGTACA               T^GTACA
StuI         1125  AGGCCT               AGG^CCT
TfiI         1050  GATTC                G^A[AT]TC
Tru9I        2899  TTAA                 T^TAA
Tsp509I      2897  AATT                 ^AATT
Van91I       1376  CCATTTCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2265  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccI         1188  GTCTAC               GT^[AC][GT]AC
             1275  GTCTAC              
Alw44I         63  GTGCAC               G^TGCAC
              780  GTGCAC              
Aor51HI      1295  AGCGCT               AGC^GCT
             1894  AGCGCT              
ApaLI          63  GTGCAC               G^TGCAC
              780  GTGCAC              
BcgI          119  CGACCTGCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1093  CGAACTCCGTGC        
BsaI          606  GAGACC               GGTCTC(1/5)
             2029  GAGACC              
BsrBI         754  GAGCGG               CCGCTC(-3/-3)
             2104  GAGCGG              
BssSI        2450  CACGAG               CACGAG(-5/-1)
             1071  CTCGTG              
BstEII        762  GGTGACC              G^GT[ACGT]ACC
             3020  GGTGACC             
BstPI         762  GGTGACC              G^GT[ACGT]ACC
             3020  GGTGACC             
BstXI        1657  CCAAGGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2195  CCAAGACATTGG        
DraIII        783  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2075  CACCGAGTG           
EagI         1878  CGGCCG               C^GGCCG
             2014  CGGCCG              
EclXI        1878  CGGCCG               C^GGCCG
             2014  CGGCCG              
Eco47III     1295  AGCGCT               AGC^GCT
             1894  AGCGCT              
Eco52I       1878  CGGCCG               C^GGCCG
             2014  CGGCCG              
EcoO65I       762  GGTGACC              G^GT[ACGT]ACC
             3020  GGTGACC             
MaeII        1531  ACGT                 A^CGT
             1709  ACGT                
SexAI        1037  ACCAGGT              A^CC[AT]GGT
             1150  ACCAGGT             
SfaNI        2084  GATGC                GCATC(5/9)
             1680  GCATC               
SfiI         1580  GGCCCTGCCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1661  GGCCATGGAGGCC       
SmlI          872  CTTGAG               C^T[CT][GA]AG
             2265  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        ApaI        AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbvI        BclI        
BcnI        BfaI        BglI        BmyI        BpmI        
BsaHI       BsaJI       BsaWI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmBI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        KasI        
KspI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       PleI        
PpuMI       RsaI        SacII       Sau3AI      Sau96I      
ScrFI       SfcI        SfoI        SmaI        StyI        
TaqI        TseI        Tsp45I      TspRI       TthHB8I     
XcmI        XhoII       XmaCI       XmaI