##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

AccIII      AclI        AgeI        Alw44I      Aor51HI     
ApaLI       AscI        AspI        AvrII       BamHI       
BbrPI       BciVI       BclI        BglI        BlnI        
BsaBI       BsaWI       BseAI       BsiWI       BsmBI       
BspDI       BspEI       BspLU11I    BssHII      BstAPI      
BstBI       ClaI        CpoI        DraIII      Eco47III    
EcoNI       FbaI        FseI        HpaI        MamI        
MfeI        MluI        MroI        MunI        NheI        
NotI        NruI        NspV        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
Psp1406I    PvuI        RsrII       SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    Tth111I     
Van91I      


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I       2015  GGTACC               G^GTACC
AflII        4196  CTTAAG               C^TTAAG
AflIII       1186  ACGTGT               A^C[GA][CT]GT
AhdI         4017  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI         4275  GGATC                GGATC(4/5)
ApaI          729  GGGCCC               GGGCC^C
AseI         2737  ATTAAT               AT^TAAT
AsnI         2737  ATTAAT               AT^TAAT
Asp718I      2015  GGTACC               G^GTACC
AspEI        4017  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1657  TGCGCA               TGC^GCA
BbeI         4369  GGCGCC               GGCGC^C
BbvCI        4397  GCTGAGG              CCTCAGC(-5/-2)
BcgI          694  GCACATCTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI         4196  CTTAAG               C^TTAAG
BglII        2838  AGATCT               A^GATCT
BsaAI         342  TACGTG               [CT]AC^GT[GA]
BsiEI        1643  CGGCCG               CG[GA][CT]^CG
BsrBI         578  GAGCGG               CCGCTC(-3/-3)
BsrDI        1116  CATTGC               GCAATG(2/0)
BssSI        1604  CACGAG               CACGAG(-5/-1)
Bst1107I     3879  GTATAC               GTA^TAC
BstEII       1847  GGTCACC              G^GT[ACGT]ACC
BstPI        1847  GGTCACC              G^GT[ACGT]ACC
BstYI        2838  AGATCT               [GA]^GATC[CT]
BstZ17I      3879  GTATAC               GTA^TAC
DrdI          599  GACTGAAGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         1643  CGGCCG               C^GGCCG
Eam1105I     4017  GACACACAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1643  CGGCCG               C^GGCCG
Eco52I       1643  CGGCCG               C^GGCCG
EcoO65I      1847  GGTCACC              G^GT[ACGT]ACC
EcoRI        3020  GAATTC               G^AATTC
EcoRV          86  GATATC               GAT^ATC
FspI         1657  TGCGCA               TGC^GCA
KasI         4369  GGCGCC               G^GCGCC
KpnI         2015  GGTACC               GGTAC^C
MflI         2838  AGATCT               [GA]^GATC[CT]
NaeI         1675  GCCGGC               GCC^GGC
NarI         4369  GGCGCC               GG^CGCC
NdeI         4155  CATATG               CA^TATG
NgoMIV       1675  GCCGGC               G^CCGGC
NspI         4112  ACATGC               [GA]CATG^[CT]
PaeR7I        211  CTCGAG               C^TCGAG
PshBI        2737  ATTAAT               AT^TAAT
SacI         1484  GAGCTC               GAGCT^C
SfoI         4369  GGCGCC               GGC^GCC
SmaI         1577  CCCGGG               CCC^GGG
SwaI         3457  ATTTAAAT             ATTT^AAAT
XhoI          211  CTCGAG               C^TCGAG
XhoII        2838  AGATCT               [GA]^GATC[CT]
XmaCI        1577  CCCGGG               C^CCGGG
XmaI         1577  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AatII         740  GACGTC               GACGT^C
             1949  GACGTC              
AlwNI         438  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              473  CAGCAGCTG           
BalI         2684  TGGCCA               TGG^CCA
             2701  TGGCCA              
BlpI          533  GCTGAGC              GC^T[ACGT]AGC
              689  GCTGAGC             
Bpu1102I      533  GCTGAGC              GC^T[ACGT]AGC
              689  GCTGAGC             
BsgI          444  CTGCAC               GTGCAG(16/14)
             4460  CTGCAC              
Bsp1407I      814  TGTACA               T^GTACA
             2484  TGTACA              
BspHI         370  TCATGA               T^CATGA
             3145  TCATGA              
BsrFI        1675  GCCGGC               [GA]^CCGG[CT]
             1936  GCCGGT              
BsrGI         814  TGTACA               T^GTACA
             2484  TGTACA              
BstXI        4248  CCAGCACTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4382  CCAGCTACTTGG        
CelII         533  GCTGAGC              GC^T[ACGT]AGC
              689  GCTGAGC             
Cfr10I       1675  GCCGGC               [GA]^CCGG[CT]
             1936  GCCGGT              
EcoT22I      2824  ATGCAT               ATGCA^T
             4114  ATGCAT              
HaeII        1678  GGCGCT               [GA]GCGC^[CT]
             4369  GGCGCC              
HindIII      1167  AAGCTT               A^AGCTT
             3631  AAGCTT              
MluNI        2684  TGGCCA               TGG^CCA
             2701  TGGCCA              
MscI         2684  TGGCCA               TGG^CCA
             2701  TGGCCA              
NcoI         1361  CCATGG               C^CATGG
             1778  CCATGG              
NsiI         2824  ATGCAT               ATGCA^T
             4114  ATGCAT              
PshAI          14  GACACAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1516  GACCCGTGTC          
RcaI          370  TCATGA               T^CATGA
             3145  TCATGA              
SalI         1855  GTCGAC               G^TCGAC
             1946  GTCGAC              
SfaNI        1867  GATGC                GCATC(5/9)
             2151  GATGC               
SspBI         814  TGTACA               T^GTACA
             2484  TGTACA              
XbaI         2712  TCTAGA               T^CTAGA
             4128  TCTAGA              
XcmI         1352  CCAGTCTCCCCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1603  CCACGAGCCCGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AluI        AocI        ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        Eco81I      EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     KspI        MaeI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        PvuII       RsaI        SacII       
Sau3AI      Sau96I      ScrFI       SfcI        SmlI        
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XmnI