################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI AspI AvrII BamHI BbrPI BciVI BclI BglI BlnI BsaBI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BssHII BstAPI BstBI ClaI CpoI DraIII Eco47III EcoNI FbaI FseI HpaI MamI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I Tth111I Van91I ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 2015 GGTACC G^GTACC AflII 4196 CTTAAG C^TTAAG AflIII 1186 ACGTGT A^C[GA][CT]GT AhdI 4017 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 4275 GGATC GGATC(4/5) ApaI 729 GGGCCC GGGCC^C AseI 2737 ATTAAT AT^TAAT AsnI 2737 ATTAAT AT^TAAT Asp718I 2015 GGTACC G^GTACC AspEI 4017 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1657 TGCGCA TGC^GCA BbeI 4369 GGCGCC GGCGC^C BbvCI 4397 GCTGAGG CCTCAGC(-5/-2) BcgI 694 GCACATCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 4196 CTTAAG C^TTAAG BglII 2838 AGATCT A^GATCT BsaAI 342 TACGTG [CT]AC^GT[GA] BsiEI 1643 CGGCCG CG[GA][CT]^CG BsrBI 578 GAGCGG CCGCTC(-3/-3) BsrDI 1116 CATTGC GCAATG(2/0) BssSI 1604 CACGAG CACGAG(-5/-1) Bst1107I 3879 GTATAC GTA^TAC BstEII 1847 GGTCACC G^GT[ACGT]ACC BstPI 1847 GGTCACC G^GT[ACGT]ACC BstYI 2838 AGATCT [GA]^GATC[CT] BstZ17I 3879 GTATAC GTA^TAC DrdI 599 GACTGAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 1643 CGGCCG C^GGCCG Eam1105I 4017 GACACACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1643 CGGCCG C^GGCCG Eco52I 1643 CGGCCG C^GGCCG EcoO65I 1847 GGTCACC G^GT[ACGT]ACC EcoRI 3020 GAATTC G^AATTC EcoRV 86 GATATC GAT^ATC FspI 1657 TGCGCA TGC^GCA KasI 4369 GGCGCC G^GCGCC KpnI 2015 GGTACC GGTAC^C MflI 2838 AGATCT [GA]^GATC[CT] NaeI 1675 GCCGGC GCC^GGC NarI 4369 GGCGCC GG^CGCC NdeI 4155 CATATG CA^TATG NgoMIV 1675 GCCGGC G^CCGGC NspI 4112 ACATGC [GA]CATG^[CT] PaeR7I 211 CTCGAG C^TCGAG PshBI 2737 ATTAAT AT^TAAT SacI 1484 GAGCTC GAGCT^C SfoI 4369 GGCGCC GGC^GCC SmaI 1577 CCCGGG CCC^GGG SwaI 3457 ATTTAAAT ATTT^AAAT XhoI 211 CTCGAG C^TCGAG XhoII 2838 AGATCT [GA]^GATC[CT] XmaCI 1577 CCCGGG C^CCGGG XmaI 1577 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AatII 740 GACGTC GACGT^C 1949 GACGTC AlwNI 438 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 473 CAGCAGCTG BalI 2684 TGGCCA TGG^CCA 2701 TGGCCA BlpI 533 GCTGAGC GC^T[ACGT]AGC 689 GCTGAGC Bpu1102I 533 GCTGAGC GC^T[ACGT]AGC 689 GCTGAGC BsgI 444 CTGCAC GTGCAG(16/14) 4460 CTGCAC Bsp1407I 814 TGTACA T^GTACA 2484 TGTACA BspHI 370 TCATGA T^CATGA 3145 TCATGA BsrFI 1675 GCCGGC [GA]^CCGG[CT] 1936 GCCGGT BsrGI 814 TGTACA T^GTACA 2484 TGTACA BstXI 4248 CCAGCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4382 CCAGCTACTTGG CelII 533 GCTGAGC GC^T[ACGT]AGC 689 GCTGAGC Cfr10I 1675 GCCGGC [GA]^CCGG[CT] 1936 GCCGGT EcoT22I 2824 ATGCAT ATGCA^T 4114 ATGCAT HaeII 1678 GGCGCT [GA]GCGC^[CT] 4369 GGCGCC HindIII 1167 AAGCTT A^AGCTT 3631 AAGCTT MluNI 2684 TGGCCA TGG^CCA 2701 TGGCCA MscI 2684 TGGCCA TGG^CCA 2701 TGGCCA NcoI 1361 CCATGG C^CATGG 1778 CCATGG NsiI 2824 ATGCAT ATGCA^T 4114 ATGCAT PshAI 14 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1516 GACCCGTGTC RcaI 370 TCATGA T^CATGA 3145 TCATGA SalI 1855 GTCGAC G^TCGAC 1946 GTCGAC SfaNI 1867 GATGC GCATC(5/9) 2151 GATGC SspBI 814 TGTACA T^GTACA 2484 TGTACA XbaI 2712 TCTAGA T^CTAGA 4128 TCTAGA XcmI 1352 CCAGTCTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1603 CCACGAGCCCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AocI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI