##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

AccII       AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AspEI       AspI        AviII       
AvrII       BbeI        BbrPI       BcgI        BfrI        
BglI        BlnI        BsaAI       BsaBI       BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BspDI       
BspEI       BspLU11I    BspMI       BsrFI       BssHII      
BssSI       BstAPI      BstBI       BstEII      BstPI       
BstUI       Cfr10I      ClaI        CpoI        DraII       
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO109I    EcoO65I     EcoRI       EcoRV       
FseI        FspI        HgaI        HpaI        KasI        
KspI        MamI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SwaI        
Tth111I     Van91I      XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AocI         2594  CCTGAGG              CC^T[ACGT]AGG
AvaI          637  CCCGGG               C^[CT]CG[GA]G
BalI          922  TGGCCA               TGG^CCA
BanII        1331  GGGCTC               G[GA]GC[CT]^C
BbvCI         245  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1582  GTATCC               GTATCC(6/5)
BsmBI        3646  CGTCTC               CGTCTC(1/5)
BsoBI         637  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     3775  TGTACA               T^GTACA
BspHI         217  TCATGA               T^CATGA
BsrBI        1378  CCGCTC               CCGCTC(-3/-3)
BsrGI        3775  TGTACA               T^GTACA
Bsu36I       2594  CCTGAGG              CC^T[ACGT]AGG
CfoI           64  GCGC                 GCG^C
DrdI         3013  GACAGGAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          977  CCATGG               C^C[GA][CT]GG
EaeI          922  TGGCCA               [CT]^GGCC[GA]
Eco81I       2594  CCTGAGG              CC^T[ACGT]AGG
FauI          408  GCGGG                CCCGC(4/6)
HaeII          63  GGCGCT               [GA]GCGC^[CT]
HapII         638  CCGG                 C^CGG
HhaI           64  GCGC                 GCG^C
HinP1I         64  GCGC                 G^CGC
HincII        456  GTCAAC               GT[CT]^[GA]AC
HindII        456  GTCAAC               GT[CT]^[GA]AC
HindIII       498  AAGCTT               A^AGCTT
HpaII         638  CCGG                 C^CGG
MluNI         922  TGGCCA               TGG^CCA
MscI          922  TGGCCA               TGG^CCA
MspI          638  CCGG                 C^CGG
NcoI          977  CCATGG               C^CATGG
PshAI         449  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          217  TCATGA               T^CATGA
SmaI          637  CCCGGG               CCC^GGG
SspBI        3775  TGTACA               T^GTACA
TaqI         3363  TCGA                 T^CGA
TthHB8I      3363  TCGA                 T^CGA
XmaCI         637  CCCGGG               C^CCGGG
XmaI          637  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AatII        1592  GACGTC               GACGT^C
             2758  GACGTC              
Acc65I       1063  GGTACC               G^GTACC
             2457  GGTACC              
AcyI         1592  GACGTC               G[GA]^CG[CT]C
             2758  GACGTC              
AseI         2507  ATTAAT               AT^TAAT
             3973  ATTAAT              
AsnI         2507  ATTAAT               AT^TAAT
             3973  ATTAAT              
Asp700I       393  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2656  GAATGCATTC          
Asp718I      1063  GGTACC               G^GTACC
             2457  GGTACC              
AspHI         548  GAGCAC               G[AT]GC[AT]^C
             1548  GAGCAC              
BamHI        1559  GGATCC               G^GATCC
             1638  GGATCC              
BclI           76  TGATCA               T^GATCA
             2819  TGATCA              
BcnI          637  CCCGG                CC^[GC]GG
              638  CCGGG               
BlpI         1668  GCTAAGC              GC^T[ACGT]AGC
             2765  GCTCAGC             
Bpu1102I     1668  GCTAAGC              GC^T[ACGT]AGC
             2765  GCTCAGC             
BsaHI        1592  GACGTC               G[GA]^CG[CT]C
             2758  GACGTC              
BsiHKAI       548  GAGCAC               G[AT]GC[AT]^C
             1548  GAGCAC              
BstXI         310  CCATGAGGTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1769  CCAATCAGCTGG        
CelII        1668  GCTAAGC              GC^T[ACGT]AGC
             2765  GCTCAGC             
EcoNI        2461  CCTAAAATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             2665  CCTCTGTCAGG         
EcoT14I       977  CCATGG               C^C[AT][AT]GG
             1963  CCTTGG              
EcoT22I       469  ATGCAT               ATGCA^T
             2658  ATGCAT              
FbaI           76  TGATCA               T^GATCA
             2819  TGATCA              
Hin1I        1592  GACGTC               G[GA]^CG[CT]C
             2758  GACGTC              
KpnI         1063  GGTACC               GGTAC^C
             2457  GGTACC              
MaeII        1593  ACGT                 A^CGT
             2759  ACGT                
NciI          637  CCCGG                CC^[GC]GG
              638  CCGGG               
NdeI         3223  CATATG               CA^TATG
             3779  CATATG              
NsiI          469  ATGCAT               ATGCA^T
             2658  ATGCAT              
PshBI        2507  ATTAAT               AT^TAAT
             3973  ATTAAT              
SmlI          580  CTCAAG               C^T[CT][GA]AG
             1713  CTCAAG              
StuI          185  AGGCCT               AGG^CCT
             1912  AGGCCT              
StyI          977  CCATGG               C^C[AT][AT]GG
             1963  CCTTGG              
XcmI           37  CCAATCAAGTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1067  CCAGTTCTTTTGTGG     
XmnI          393  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2656  GAATGCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaII       BanI        
BbsI        BbvI        BfaI        BglII       BmyI        
BpmI        BpuAI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
Bsp1286I    BsrDI       BsrI        BssKI       Bst1107I    
BstF5I      BstNI       BstYI       BstZ17I     Cac8I       
Cfr13I      DdeI        DpnII       DraI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        PstI        
PvuII       RsaI        SapI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SphI        SspI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII