################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AspEI AspI AviII AvrII BbeI BbrPI BcgI BfrI BglI BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BspDI BspEI BspLU11I BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Cfr10I ClaI CpoI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV FseI FspI HgaI HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AocI 2594 CCTGAGG CC^T[ACGT]AGG AvaI 637 CCCGGG C^[CT]CG[GA]G BalI 922 TGGCCA TGG^CCA BanII 1331 GGGCTC G[GA]GC[CT]^C BbvCI 245 GCTGAGG CCTCAGC(-5/-2) BciVI 1582 GTATCC GTATCC(6/5) BsmBI 3646 CGTCTC CGTCTC(1/5) BsoBI 637 CCCGGG C^[CT]CG[GA]G Bsp1407I 3775 TGTACA T^GTACA BspHI 217 TCATGA T^CATGA BsrBI 1378 CCGCTC CCGCTC(-3/-3) BsrGI 3775 TGTACA T^GTACA Bsu36I 2594 CCTGAGG CC^T[ACGT]AGG CfoI 64 GCGC GCG^C DrdI 3013 GACAGGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 977 CCATGG C^C[GA][CT]GG EaeI 922 TGGCCA [CT]^GGCC[GA] Eco81I 2594 CCTGAGG CC^T[ACGT]AGG FauI 408 GCGGG CCCGC(4/6) HaeII 63 GGCGCT [GA]GCGC^[CT] HapII 638 CCGG C^CGG HhaI 64 GCGC GCG^C HinP1I 64 GCGC G^CGC HincII 456 GTCAAC GT[CT]^[GA]AC HindII 456 GTCAAC GT[CT]^[GA]AC HindIII 498 AAGCTT A^AGCTT HpaII 638 CCGG C^CGG MluNI 922 TGGCCA TGG^CCA MscI 922 TGGCCA TGG^CCA MspI 638 CCGG C^CGG NcoI 977 CCATGG C^CATGG PshAI 449 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 217 TCATGA T^CATGA SmaI 637 CCCGGG CCC^GGG SspBI 3775 TGTACA T^GTACA TaqI 3363 TCGA T^CGA TthHB8I 3363 TCGA T^CGA XmaCI 637 CCCGGG C^CCGGG XmaI 637 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 1592 GACGTC GACGT^C 2758 GACGTC Acc65I 1063 GGTACC G^GTACC 2457 GGTACC AcyI 1592 GACGTC G[GA]^CG[CT]C 2758 GACGTC AseI 2507 ATTAAT AT^TAAT 3973 ATTAAT AsnI 2507 ATTAAT AT^TAAT 3973 ATTAAT Asp700I 393 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2656 GAATGCATTC Asp718I 1063 GGTACC G^GTACC 2457 GGTACC AspHI 548 GAGCAC G[AT]GC[AT]^C 1548 GAGCAC BamHI 1559 GGATCC G^GATCC 1638 GGATCC BclI 76 TGATCA T^GATCA 2819 TGATCA BcnI 637 CCCGG CC^[GC]GG 638 CCGGG BlpI 1668 GCTAAGC GC^T[ACGT]AGC 2765 GCTCAGC Bpu1102I 1668 GCTAAGC GC^T[ACGT]AGC 2765 GCTCAGC BsaHI 1592 GACGTC G[GA]^CG[CT]C 2758 GACGTC BsiHKAI 548 GAGCAC G[AT]GC[AT]^C 1548 GAGCAC BstXI 310 CCATGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1769 CCAATCAGCTGG CelII 1668 GCTAAGC GC^T[ACGT]AGC 2765 GCTCAGC EcoNI 2461 CCTAAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2665 CCTCTGTCAGG EcoT14I 977 CCATGG C^C[AT][AT]GG 1963 CCTTGG EcoT22I 469 ATGCAT ATGCA^T 2658 ATGCAT FbaI 76 TGATCA T^GATCA 2819 TGATCA Hin1I 1592 GACGTC G[GA]^CG[CT]C 2758 GACGTC KpnI 1063 GGTACC GGTAC^C 2457 GGTACC MaeII 1593 ACGT A^CGT 2759 ACGT NciI 637 CCCGG CC^[GC]GG 638 CCGGG NdeI 3223 CATATG CA^TATG 3779 CATATG NsiI 469 ATGCAT ATGCA^T 2658 ATGCAT PshBI 2507 ATTAAT AT^TAAT 3973 ATTAAT SmlI 580 CTCAAG C^T[CT][GA]AG 1713 CTCAAG StuI 185 AGGCCT AGG^CCT 1912 AGGCCT StyI 977 CCATGG C^C[AT][AT]GG 1963 CCTTGG XcmI 37 CCAATCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1067 CCAGTTCTTTTGTGG XmnI 393 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2656 GAATGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanI BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SphI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII