##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AccIII      AclI        AflII       AflIII      AgeI        
AhdI        AscI        AspEI       BamHI       BbeI        
BbrPI       BbsI        BcgI        BciVI       BclI        
BfrI        BglII       BpuAI       BsaAI       BsaBI       
BseAI       BsiEI       BsiWI       BsmBI       BspDI       
BspEI       BspHI       BspLU11I    BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     ClaI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoNI       EcoRV       FbaI        FseI        HincII      
HindII      HpaI        KasI        MamI        MfeI        
MluI        MroI        MunI        NarI        NcoI        
NdeI        NotI        NruI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        Psp1406I    PvuI        RcaI        SacI        
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SnaBI       SphI        Sse8387I    
SwaI        Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   60 enzymes) #
###################################

AatII         337  GACGTC               GACGT^C
AccI         2686  GTAGAC               GT^[AC][GT]AC
AcyI          337  GACGTC               G[GA]^CG[CT]C
Alw44I        579  GTGCAC               G^TGCAC
Aor51HI      2849  AGCGCT               AGC^GCT
ApaLI         579  GTGCAC               G^TGCAC
AseI         3606  ATTAAT               AT^TAAT
AsnI         3606  ATTAAT               AT^TAAT
AspI          204  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         264  TGCGCA               TGC^GCA
AvrII        1513  CCTAGG               C^CTAGG
BglI         1999  GCCAAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         1513  CCTAGG               C^CTAGG
BlpI         1693  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1693  GCTGAGC              GC^T[ACGT]AGC
BsaHI         337  GACGTC               G[GA]^CG[CT]C
BsaI          310  GGTCTC               GGTCTC(1/5)
BsaWI        1475  ACCGGA               [AT]^CCGG[AT]
BsmI         3084  GAATGC               GAATGC(1/-1)
Bsp1407I     2648  TGTACA               T^GTACA
BsrBI         844  CCGCTC               CCGCTC(-3/-3)
BsrDI        3013  GCAATG               GCAATG(2/0)
BsrGI        2648  TGTACA               T^GTACA
BstAPI       1704  GCAGTTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1518  GGTAACC              G^GT[ACGT]ACC
BstPI        1518  GGTAACC              G^GT[ACGT]ACC
CelII        1693  GCTGAGC              GC^T[ACGT]AGC
CpoI         1401  CGGACCG              CG^G[AT]CCG
DraIII        815  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1924  GAAGAG               CTCTTC(1/4)
Eco47III     2849  AGCGCT               AGC^GCT
EcoO65I      1518  GGTAACC              G^GT[ACGT]ACC
EcoT22I      3086  ATGCAT               ATGCA^T
FspI          264  TGCGCA               TGC^GCA
HgaI          591  GACGC                GACGC(5/10)
Hin1I         337  GACGTC               G[GA]^CG[CT]C
Ksp632I      1924  GAAGAG               CTCTTC(1/4)
KspI         1068  CCGCGG               CCGC^GG
NaeI          987  GCCGGC               GCC^GGC
NgoMIV        987  GCCGGC               G^CCGGC
NheI          101  GCTAGC               G^CTAGC
NsiI         3086  ATGCAT               ATGCA^T
NspI         2820  GCATGT               [GA]CATG^[CT]
PflFI         204  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2084  GACTTCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3606  ATTAAT               AT^TAAT
PvuII         276  CAGCTG               CAG^CTG
RsrII        1401  CGGACCG              CG^G[AT]CCG
SacII        1068  CCGCGG               CCGC^GG
SexAI         816  ACCTGGT              A^CC[AT]GGT
SgrAI         191  CGCCGGTG             C[GA]^CCGG[CT]G
SmaI         1643  CCCGGG               CCC^GGG
SpeI         2610  ACTAGT               A^CTAGT
SspBI        2648  TGTACA               T^GTACA
SspI         1977  AATATT               AAT^ATT
Tsp45I        589  GTGAC                ^GT[GC]AC
Tth111I       204  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3371  TCTAGA               T^CTAGA
XmaCI        1643  CCCGGG               C^CCGGG
XmaI         1643  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

Acc65I        681  GGTACC               G^GTACC
             2367  GGTACC              
AocI         1555  CCTAAGG              CC^T[ACGT]AGG
             1864  CCTGAGG             
Asp700I      3236  GAAATGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3346  GAAATCTTTC          
Asp718I       681  GGTACC               G^GTACC
             2367  GGTACC              
AvaI         1440  CCCGAG               C^[CT]CG[GA]G
             1643  CCCGGG              
BbvCI         424  GCTGAGG              CCTCAGC(-5/-2)
             2305  GCTGAGG             
BseRI        1219  GAGGAG               GAGGAG(10/8)
             2677  GAGGAG              
BsoBI        1440  CCCGAG               C^[CT]CG[GA]G
             1643  CCCGGG              
BspMI        2830  ACCTGC               ACCTGC(4/8)
             2724  GCAGGT              
BsrFI         192  GCCGGT               [GA]^CCGG[CT]
              987  GCCGGC              
BstF5I       2372  CATCC                GGATG(2/0)
             2033  GGATG               
Bsu36I       1555  CCTAAGG              CC^T[ACGT]AGG
             1864  CCTGAGG             
Cfr10I        192  GCCGGT               [GA]^CCGG[CT]
              987  GCCGGC              
Eco81I       1555  CCTAAGG              CC^T[ACGT]AGG
             1864  CCTGAGG             
FokI         2372  CATCC                GGATG(9/13)
             2033  GGATG               
HaeII         472  AGCGCC               [GA]GCGC^[CT]
             2849  AGCGCT              
HindIII      2733  AAGCTT               A^AGCTT
             3419  AAGCTT              
KpnI          681  GGTACC               GGTAC^C
             2367  GGTACC              
MaeII         338  ACGT                 A^CGT
             1688  ACGT                
SmlI          468  CTCAAG               C^T[CT][GA]AG
             2841  CTTGAG              
TaqI         1153  TCGA                 T^CGA
             1583  TCGA                
TthHB8I      1153  TCGA                 T^CGA
             1583  TCGA                
XcmI         1523  CCAAGGCCTCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1616  CCATTAGTATTATGG     
XmnI         3236  GAAATGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3346  GAAATCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   91 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApaI        ApoI        AspHI       
AvaII       BalI        BanI        BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EcoO109I    EcoRI       EcoRII      EcoT14I     
FauI        Fnu4HI      HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StuI        StyI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
XhoII