################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AccIII AclI AflII AflIII AgeI AhdI AscI AspEI BamHI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglII BpuAI BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I ClaI DraI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoRV FbaI FseI HincII HindII HpaI KasI MamI MfeI MluI MroI MunI NarI NcoI NdeI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SnaBI SphI Sse8387I SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 337 GACGTC GACGT^C AccI 2686 GTAGAC GT^[AC][GT]AC AcyI 337 GACGTC G[GA]^CG[CT]C Alw44I 579 GTGCAC G^TGCAC Aor51HI 2849 AGCGCT AGC^GCT ApaLI 579 GTGCAC G^TGCAC AseI 3606 ATTAAT AT^TAAT AsnI 3606 ATTAAT AT^TAAT AspI 204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 264 TGCGCA TGC^GCA AvrII 1513 CCTAGG C^CTAGG BglI 1999 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1513 CCTAGG C^CTAGG BlpI 1693 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1693 GCTGAGC GC^T[ACGT]AGC BsaHI 337 GACGTC G[GA]^CG[CT]C BsaI 310 GGTCTC GGTCTC(1/5) BsaWI 1475 ACCGGA [AT]^CCGG[AT] BsmI 3084 GAATGC GAATGC(1/-1) Bsp1407I 2648 TGTACA T^GTACA BsrBI 844 CCGCTC CCGCTC(-3/-3) BsrDI 3013 GCAATG GCAATG(2/0) BsrGI 2648 TGTACA T^GTACA BstAPI 1704 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1518 GGTAACC G^GT[ACGT]ACC BstPI 1518 GGTAACC G^GT[ACGT]ACC CelII 1693 GCTGAGC GC^T[ACGT]AGC CpoI 1401 CGGACCG CG^G[AT]CCG DraIII 815 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 1924 GAAGAG CTCTTC(1/4) Eco47III 2849 AGCGCT AGC^GCT EcoO65I 1518 GGTAACC G^GT[ACGT]ACC EcoT22I 3086 ATGCAT ATGCA^T FspI 264 TGCGCA TGC^GCA HgaI 591 GACGC GACGC(5/10) Hin1I 337 GACGTC G[GA]^CG[CT]C Ksp632I 1924 GAAGAG CTCTTC(1/4) KspI 1068 CCGCGG CCGC^GG NaeI 987 GCCGGC GCC^GGC NgoMIV 987 GCCGGC G^CCGGC NheI 101 GCTAGC G^CTAGC NsiI 3086 ATGCAT ATGCA^T NspI 2820 GCATGT [GA]CATG^[CT] PflFI 204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 2084 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3606 ATTAAT AT^TAAT PvuII 276 CAGCTG CAG^CTG RsrII 1401 CGGACCG CG^G[AT]CCG SacII 1068 CCGCGG CCGC^GG SexAI 816 ACCTGGT A^CC[AT]GGT SgrAI 191 CGCCGGTG C[GA]^CCGG[CT]G SmaI 1643 CCCGGG CCC^GGG SpeI 2610 ACTAGT A^CTAGT SspBI 2648 TGTACA T^GTACA SspI 1977 AATATT AAT^ATT Tsp45I 589 GTGAC ^GT[GC]AC Tth111I 204 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3371 TCTAGA T^CTAGA XmaCI 1643 CCCGGG C^CCGGG XmaI 1643 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 681 GGTACC G^GTACC 2367 GGTACC AocI 1555 CCTAAGG CC^T[ACGT]AGG 1864 CCTGAGG Asp700I 3236 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3346 GAAATCTTTC Asp718I 681 GGTACC G^GTACC 2367 GGTACC AvaI 1440 CCCGAG C^[CT]CG[GA]G 1643 CCCGGG BbvCI 424 GCTGAGG CCTCAGC(-5/-2) 2305 GCTGAGG BseRI 1219 GAGGAG GAGGAG(10/8) 2677 GAGGAG BsoBI 1440 CCCGAG C^[CT]CG[GA]G 1643 CCCGGG BspMI 2830 ACCTGC ACCTGC(4/8) 2724 GCAGGT BsrFI 192 GCCGGT [GA]^CCGG[CT] 987 GCCGGC BstF5I 2372 CATCC GGATG(2/0) 2033 GGATG Bsu36I 1555 CCTAAGG CC^T[ACGT]AGG 1864 CCTGAGG Cfr10I 192 GCCGGT [GA]^CCGG[CT] 987 GCCGGC Eco81I 1555 CCTAAGG CC^T[ACGT]AGG 1864 CCTGAGG FokI 2372 CATCC GGATG(9/13) 2033 GGATG HaeII 472 AGCGCC [GA]GCGC^[CT] 2849 AGCGCT HindIII 2733 AAGCTT A^AGCTT 3419 AAGCTT KpnI 681 GGTACC GGTAC^C 2367 GGTACC MaeII 338 ACGT A^CGT 1688 ACGT SmlI 468 CTCAAG C^T[CT][GA]AG 2841 CTTGAG TaqI 1153 TCGA T^CGA 1583 TCGA TthHB8I 1153 TCGA T^CGA 1583 TCGA XcmI 1523 CCAAGGCCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1616 CCATTAGTATTATGG XmnI 3236 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3346 GAAATCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII