##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AhdI        AscI        Asp718I     AspEI       AvaI        
AviII       AvrII       BbrPI       BciVI       BclI        
BfrI        BglII       BlnI        BsaAI       BsiWI       
BsmBI       BsoBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstZ17I     ClaI        Eam1105I    
EcoO65I     EcoRI       EcoRV       FbaI        FseI        
FspI        KpnI        KspI        MluI        NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SwaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   59 enzymes) #
###################################

AccI          804  GTCTAC               GT^[AC][GT]AC
AccII        1447  CGCG                 CG^CG
AccIII       3418  TCCGGA               T^CCGGA
AgeI         1587  ACCGGT               A^CCGGT
Alw44I       3133  GTGCAC               G^TGCAC
AlwNI        1226  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          955  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       677  AGCGCT               AGC^GCT
ApaI         1522  GGGCCC               GGGCC^C
ApaLI        3133  GTGCAC               G^TGCAC
AseI         2298  ATTAAT               AT^TAAT
AsnI         2298  ATTAAT               AT^TAAT
Asp700I      3622  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         2601  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        1095  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2350  GCAAGAGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          335  CCGGG                CC^[GC]GG
BsaBI        2443  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         3123  GGTCTC               GGTCTC(1/5)
BseAI        3418  TCCGGA               T^CCGGA
BsiEI         574  CGGCCG               CG[GA][CT]^CG
BspEI        3418  TCCGGA               T^CCGGA
BsrBI        1514  GAGCGG               CCGCTC(-3/-3)
BssSI        2886  CTCGTG               CACGAG(-5/-1)
BstAPI       1225  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1447  CGCG                 CG^CG
BstXI         879  CCAGGGAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        955  CCTCAGG              CC^T[ACGT]AGG
CpoI         1584  CGGACCG              CG^G[AT]CCG
DraIII       1750  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1582  GACGGACCGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          574  CGGCCG               C^GGCCG
EclXI         574  CGGCCG               C^GGCCG
Eco47III      677  AGCGCT               AGC^GCT
Eco52I        574  CGGCCG               C^GGCCG
Eco81I        955  CCTCAGG              CC^T[ACGT]AGG
HgaI         2801  GCGTC                GACGC(5/10)
HpaI         3236  GTTAAC               GTT^AAC
MamI         2443  GATAGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         3418  TCCGGA               T^CCGGA
MvnI         1447  CGCG                 CG^CG
NaeI         1090  GCCGGC               GCC^GGC
NciI          335  CCGGG                CC^[GC]GG
NgoMIV       1090  GCCGGC               G^CCGGC
PflFI        2601  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        1587  ACCGGT               A^CCGGT
PpuMI        1707  GGGTCCT              [GA]G^G[AT]CC[CT]
PshBI        2298  ATTAAT               AT^TAAT
RsrII        1584  CGGACCG              CG^G[AT]CCG
SacI         3266  GAGCTC               GAGCT^C
SapI         3327  GAAGAGC              GCTCTTC(1/4)
SmlI         2541  CTTGAG               C^T[CT][GA]AG
SspI         3813  AATATT               AAT^ATT
StuI         1691  AGGCCT               AGG^CCT
TaqI         1200  TCGA                 T^CGA
Tth111I      2601  GACCTAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I      1200  TCGA                 T^CGA
XbaI         1726  TCTAGA               T^CTAGA
XmnI         3622  GAAATCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI          496  GGCGCC               G[GA]^CG[CT]C
             1423  GGCGCC              
BamHI         547  GGATCC               G^GATCC
             3320  GGATCC              
BbeI          496  GGCGCC               GGCGC^C
             1423  GGCGCC              
BbsI         2598  GAAGAC               GAAGAC(2/6)
             3437  GAAGAC              
BglI          683  GCCAGGATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1210  GCCCCTCTGGC         
BpuAI        2598  GAAGAC               GAAGAC(2/6)
             3437  GAAGAC              
BsaHI         496  GGCGCC               G[GA]^CG[CT]C
             1423  GGCGCC              
BsaWI        1587  ACCGGT               [AT]^CCGG[AT]
             3418  TCCGGA              
BsmI         1083  GAATGC               GAATGC(1/-1)
             2376  GAATGC              
BspMI        1146  ACCTGC               ACCTGC(4/8)
             2828  ACCTGC              
BsrFI        1090  GCCGGC               [GA]^CCGG[CT]
             1587  ACCGGT              
Cfr10I       1090  GCCGGC               [GA]^CCGG[CT]
             1587  ACCGGT              
EcoNI          58  CCTGTGCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1617  CCTGGCCCAGG         
Hin1I         496  GGCGCC               G[GA]^CG[CT]C
             1423  GGCGCC              
HindIII      1896  AAGCTT               A^AGCTT
             1935  AAGCTT              
KasI          496  GGCGCC               G^GCGCC
             1423  GGCGCC              
NarI          496  GGCGCC               GG^CGCC
             1423  GGCGCC              
NdeI            5  CATATG               CA^TATG
             1911  CATATG              
PflMI         213  CCACTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1537  CCAGGAGGTGG         
PshAI         834  GACCATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3117  GACCAAGGTC          
PstI          300  CTGCAG               CTGCA^G
             1598  CTGCAG              
SfoI          496  GGCGCC               GGC^GCC
             1423  GGCGCC              
SphI         2832  GCATGC               GCATG^C
             3159  GCATGC              
Van91I        213  CCACTCCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1537  CCAGGAGGTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BalI        BanI        
BanII       BbvI        BfaI        BlpI        BmyI        
BpmI        Bpu1102I    BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     EcoT22I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MfeI        MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MunI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XcmI        XhoII