################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AhdI AscI Asp718I AspEI AvaI AviII AvrII BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI Eam1105I EcoO65I EcoRI EcoRV FbaI FseI FspI KpnI KspI MluI NheI NotI NruI NspV PacI PaeR7I PmaCI PmeI PmlI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccI 804 GTCTAC GT^[AC][GT]AC AccII 1447 CGCG CG^CG AccIII 3418 TCCGGA T^CCGGA AgeI 1587 ACCGGT A^CCGGT Alw44I 3133 GTGCAC G^TGCAC AlwNI 1226 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 955 CCTCAGG CC^T[ACGT]AGG Aor51HI 677 AGCGCT AGC^GCT ApaI 1522 GGGCCC GGGCC^C ApaLI 3133 GTGCAC G^TGCAC AseI 2298 ATTAAT AT^TAAT AsnI 2298 ATTAAT AT^TAAT Asp700I 3622 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2601 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1095 CCTCAGC CCTCAGC(-5/-2) BcgI 2350 GCAAGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 335 CCGGG CC^[GC]GG BsaBI 2443 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 3123 GGTCTC GGTCTC(1/5) BseAI 3418 TCCGGA T^CCGGA BsiEI 574 CGGCCG CG[GA][CT]^CG BspEI 3418 TCCGGA T^CCGGA BsrBI 1514 GAGCGG CCGCTC(-3/-3) BssSI 2886 CTCGTG CACGAG(-5/-1) BstAPI 1225 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1447 CGCG CG^CG BstXI 879 CCAGGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 955 CCTCAGG CC^T[ACGT]AGG CpoI 1584 CGGACCG CG^G[AT]CCG DraIII 1750 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1582 GACGGACCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 574 CGGCCG C^GGCCG EclXI 574 CGGCCG C^GGCCG Eco47III 677 AGCGCT AGC^GCT Eco52I 574 CGGCCG C^GGCCG Eco81I 955 CCTCAGG CC^T[ACGT]AGG HgaI 2801 GCGTC GACGC(5/10) HpaI 3236 GTTAAC GTT^AAC MamI 2443 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3418 TCCGGA T^CCGGA MvnI 1447 CGCG CG^CG NaeI 1090 GCCGGC GCC^GGC NciI 335 CCGGG CC^[GC]GG NgoMIV 1090 GCCGGC G^CCGGC PflFI 2601 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 1587 ACCGGT A^CCGGT PpuMI 1707 GGGTCCT [GA]G^G[AT]CC[CT] PshBI 2298 ATTAAT AT^TAAT RsrII 1584 CGGACCG CG^G[AT]CCG SacI 3266 GAGCTC GAGCT^C SapI 3327 GAAGAGC GCTCTTC(1/4) SmlI 2541 CTTGAG C^T[CT][GA]AG SspI 3813 AATATT AAT^ATT StuI 1691 AGGCCT AGG^CCT TaqI 1200 TCGA T^CGA Tth111I 2601 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1200 TCGA T^CGA XbaI 1726 TCTAGA T^CTAGA XmnI 3622 GAAATCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcyI 496 GGCGCC G[GA]^CG[CT]C 1423 GGCGCC BamHI 547 GGATCC G^GATCC 3320 GGATCC BbeI 496 GGCGCC GGCGC^C 1423 GGCGCC BbsI 2598 GAAGAC GAAGAC(2/6) 3437 GAAGAC BglI 683 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1210 GCCCCTCTGGC BpuAI 2598 GAAGAC GAAGAC(2/6) 3437 GAAGAC BsaHI 496 GGCGCC G[GA]^CG[CT]C 1423 GGCGCC BsaWI 1587 ACCGGT [AT]^CCGG[AT] 3418 TCCGGA BsmI 1083 GAATGC GAATGC(1/-1) 2376 GAATGC BspMI 1146 ACCTGC ACCTGC(4/8) 2828 ACCTGC BsrFI 1090 GCCGGC [GA]^CCGG[CT] 1587 ACCGGT Cfr10I 1090 GCCGGC [GA]^CCGG[CT] 1587 ACCGGT EcoNI 58 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1617 CCTGGCCCAGG Hin1I 496 GGCGCC G[GA]^CG[CT]C 1423 GGCGCC HindIII 1896 AAGCTT A^AGCTT 1935 AAGCTT KasI 496 GGCGCC G^GCGCC 1423 GGCGCC NarI 496 GGCGCC GG^CGCC 1423 GGCGCC NdeI 5 CATATG CA^TATG 1911 CATATG PflMI 213 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1537 CCAGGAGGTGG PshAI 834 GACCATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3117 GACCAAGGTC PstI 300 CTGCAG CTGCA^G 1598 CTGCAG SfoI 496 GGCGCC GGC^GCC 1423 GGCGCC SphI 2832 GCATGC GCATG^C 3159 GCATGC Van91I 213 CCACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1537 CCAGGAGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BalI BanI BanII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MluNI MnlI MscI MseI MslI MspA1I MspI MunI MvaI MwoI NcoI NdeII NlaIII NlaIV NsiI NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI XhoII