################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AhdI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BciVI BclI BfrI BglII BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SexAI SfuI SnaBI SpeI SspBI SspI SwaI Tth111I XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 4228 TCCGGA T^CCGGA AflIII 4695 ACGTGT A^C[GA][CT]GT AgeI 2666 ACCGGT A^CCGGT AscI 1024 GGCGCGCC GG^CGCGCC AviII 1160 TGCGCA TGC^GCA BalI 2492 TGGCCA TGG^CCA BamHI 3169 GGATCC G^GATCC BbsI 1874 GTCTTC GAAGAC(2/6) BcgI 4379 CGAGACCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1874 GTCTTC GAAGAC(2/6) BseAI 4228 TCCGGA T^CCGGA BspEI 4228 TCCGGA T^CCGGA BspHI 1176 TCATGA T^CATGA BsrDI 3247 GCAATG GCAATG(2/0) BstAPI 796 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1991 GGTCACC G^GT[ACGT]ACC BstPI 1991 GGTCACC G^GT[ACGT]ACC DraIII 2330 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1991 GGTCACC G^GT[ACGT]ACC FspI 1160 TGCGCA TGC^GCA MluNI 2492 TGGCCA TGG^CCA MroI 4228 TCCGGA T^CCGGA MscI 2492 TGGCCA TGG^CCA PaeR7I 553 CTCGAG C^TCGAG PinAI 2666 ACCGGT A^CCGGT RcaI 1176 TCATGA T^CATGA SapI 910 GCTCTTC GCTCTTC(1/4) ScaI 1706 AGTACT AGT^ACT SgrAI 24 CGCCGGCG C[GA]^CCGG[CT]G Tsp509I 4702 AATT ^AATT XbaI 2929 TCTAGA T^CTAGA XhoI 553 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 357 GTCTAC GT^[AC][GT]AC 545 GTAGAC Aor51HI 1145 AGCGCT AGC^GCT 2483 AGCGCT BbvCI 3182 CCTCAGC CCTCAGC(-5/-2) 4336 GCTGAGG BssSI 1865 CACGAG CACGAG(-5/-1) 4490 CTCGTG BstYI 71 AGATCC [GA]^GATC[CT] 3169 GGATCC DrdI 2737 GACTCGCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3503 GACCCTGAGGTC Eco47III 1145 AGCGCT AGC^GCT 2483 AGCGCT HincII 220 GTCAAC GT[CT]^[GA]AC 3751 GTCAAC HindII 220 GTCAAC GT[CT]^[GA]AC 3751 GTCAAC MflI 71 AGATCC [GA]^GATC[CT] 3169 GGATCC SbfI 793 CCTGCAGG CCTGCA^GG 2901 CCTGCAGG SfiI 1084 GGCCGCTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3155 GGCCGTGAGGGCC SmaI 3466 CCCGGG CCC^GGG 4350 CCCGGG SmlI 553 CTCGAG C^T[CT][GA]AG 3993 CTCAAG Sse8387I 793 CCTGCAGG CCTGCA^GG 2901 CCTGCAGG StuI 1071 AGGCCT AGG^CCT 4022 AGGCCT TfiI 108 GATTC G^A[AT]TC 3555 GATTC XhoII 71 AGATCC [GA]^GATC[CT] 3169 GGATCC XmaCI 3466 CCCGGG C^CCGGG 4350 CCCGGG XmaI 3466 CCCGGG C^CCGGG 4350 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BanI BanII BbeI BbrPI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SphI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI