##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AhdI        ApoI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvrII       BciVI       
BclI        BfrI        BglII       BlnI        BsaBI       
BsiWI       BsmI        Bsp1407I    BspDI       BspLU11I    
BsrGI       Bst1107I    BstBI       BstZ17I     ClaI        
CpoI        DraI        EagI        Eam1105I    EclXI       
Eco52I      EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MunI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SalI        SexAI       SfuI        
SnaBI       SpeI        SspBI       SspI        SwaI        
Tth111I     XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AccIII       4228  TCCGGA               T^CCGGA
AflIII       4695  ACGTGT               A^C[GA][CT]GT
AgeI         2666  ACCGGT               A^CCGGT
AscI         1024  GGCGCGCC             GG^CGCGCC
AviII        1160  TGCGCA               TGC^GCA
BalI         2492  TGGCCA               TGG^CCA
BamHI        3169  GGATCC               G^GATCC
BbsI         1874  GTCTTC               GAAGAC(2/6)
BcgI         4379  CGAGACCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI        1874  GTCTTC               GAAGAC(2/6)
BseAI        4228  TCCGGA               T^CCGGA
BspEI        4228  TCCGGA               T^CCGGA
BspHI        1176  TCATGA               T^CATGA
BsrDI        3247  GCAATG               GCAATG(2/0)
BstAPI        796  GCAGGCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1991  GGTCACC              G^GT[ACGT]ACC
BstPI        1991  GGTCACC              G^GT[ACGT]ACC
DraIII       2330  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      1991  GGTCACC              G^GT[ACGT]ACC
FspI         1160  TGCGCA               TGC^GCA
MluNI        2492  TGGCCA               TGG^CCA
MroI         4228  TCCGGA               T^CCGGA
MscI         2492  TGGCCA               TGG^CCA
PaeR7I        553  CTCGAG               C^TCGAG
PinAI        2666  ACCGGT               A^CCGGT
RcaI         1176  TCATGA               T^CATGA
SapI          910  GCTCTTC              GCTCTTC(1/4)
ScaI         1706  AGTACT               AGT^ACT
SgrAI          24  CGCCGGCG             C[GA]^CCGG[CT]G
Tsp509I      4702  AATT                 ^AATT
XbaI         2929  TCTAGA               T^CTAGA
XhoI          553  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccI          357  GTCTAC               GT^[AC][GT]AC
              545  GTAGAC              
Aor51HI      1145  AGCGCT               AGC^GCT
             2483  AGCGCT              
BbvCI        3182  CCTCAGC              CCTCAGC(-5/-2)
             4336  GCTGAGG             
BssSI        1865  CACGAG               CACGAG(-5/-1)
             4490  CTCGTG              
BstYI          71  AGATCC               [GA]^GATC[CT]
             3169  GGATCC              
DrdI         2737  GACTCGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3503  GACCCTGAGGTC        
Eco47III     1145  AGCGCT               AGC^GCT
             2483  AGCGCT              
HincII        220  GTCAAC               GT[CT]^[GA]AC
             3751  GTCAAC              
HindII        220  GTCAAC               GT[CT]^[GA]AC
             3751  GTCAAC              
MflI           71  AGATCC               [GA]^GATC[CT]
             3169  GGATCC              
SbfI          793  CCTGCAGG             CCTGCA^GG
             2901  CCTGCAGG            
SfiI         1084  GGCCGCTGGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3155  GGCCGTGAGGGCC       
SmaI         3466  CCCGGG               CCC^GGG
             4350  CCCGGG              
SmlI          553  CTCGAG               C^T[CT][GA]AG
             3993  CTCAAG              
Sse8387I      793  CCTGCAGG             CCTGCA^GG
             2901  CCTGCAGG            
StuI         1071  AGGCCT               AGG^CCT
             4022  AGGCCT              
TfiI          108  GATTC                G^A[AT]TC
             3555  GATTC               
XhoII          71  AGATCC               [GA]^GATC[CT]
             3169  GGATCC              
XmaCI        3466  CCCGGG               C^CCGGG
             4350  CCCGGG              
XmaI         3466  CCCGGG               C^CCGGG
             4350  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  134 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwI        AlwNI       AocI        ApaI        
ApaLI       AspHI       AvaI        AvaII       BanI        
BanII       BbeI        BbrPI       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaHI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrFI       
BsrI        BssHII      BssKI       BstF5I      BstNI       
BstUI       BstXI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco81I      
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PmaCI       PmlI        PpuMI       PstI        PvuII       
RsaI        SacI        SacII       Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SfoI        SphI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI