##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        Aor51HI     AscI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BamHI       BbeI        BbrPI       BciVI       BfrI        
BglI        BlnI        BsaAI       BseAI       BsiWI       
BsoBI       Bsp1407I    BspEI       BsrGI       BssHII      
Bst1107I    BstEII      BstPI       BstXI       BstZ17I     
CpoI        DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       EcoT22I     
FseI        FspI        HpaI        KasI        KpnI        
MfeI        MluI        MroI        MunI        NarI        
NcoI        NotI        NruI        NsiI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       Psp1406I    PvuI        RsrII       
SacI        SalI        SbfI        SfiI        SfoI        
SmaI        SnaBI       SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AatII         728  GACGTC               GACGT^C
Alw44I       3963  GTGCAC               G^TGCAC
ApaI         3096  GGGCCC               GGGCC^C
ApaLI        3963  GTGCAC               G^TGCAC
BanI         3545  GGCACC               G^G[CT][GA]CC
BbvCI        2125  GCTGAGG              CCTCAGC(-5/-2)
BcgI          324  GCACAGATGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         3978  TGATCA               T^GATCA
BlpI         3239  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3239  GCTGAGC              GC^T[ACGT]AGC
BsiEI          22  CGACCG               CG[GA][CT]^CG
BsmBI        1521  GAGACG               CGTCTC(1/5)
BspDI        1451  ATCGAT               AT^CGAT
BspHI        1065  TCATGA               T^CATGA
BspLU11I     4280  ACATGT               A^CATGT
BsrFI          17  GCCGGC               [GA]^CCGG[CT]
BstAPI       2347  GCAGGTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        3239  GCTGAGC              GC^T[ACGT]AGC
Cfr10I         17  GCCGGC               [GA]^CCGG[CT]
ClaI         1451  ATCGAT               AT^CGAT
DrdI          459  GACTCTTCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        3253  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         3978  TGATCA               T^GATCA
HaeII        3085  GGCGCT               [GA]GCGC^[CT]
KspI           25  CCGCGG               CCGC^GG
NaeI           17  GCCGGC               GCC^GGC
NdeI          375  CATATG               CA^TATG
NgoMIV         17  GCCGGC               G^CCGGC
NheI         3227  GCTAGC               G^CTAGC
NspI         4280  ACATGT               [GA]CATG^[CT]
RcaI         1065  TCATGA               T^CATGA
SacII          25  CCGCGG               CCGC^GG
SgrAI          16  CGCCGGCG             C[GA]^CCGG[CT]G
SpeI         4051  ACTAGT               A^CTAGT
XbaI         2690  TCTAGA               T^CTAGA
XcmI         2301  CCAAGCTTGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   37 enzymes) #
###################################

AcyI          728  GACGTC               G[GA]^CG[CT]C
             3459  GACGCC              
AflIII        544  ACGTGT               A^C[GA][CT]GT
             4280  ACATGT              
AocI         1393  CCTCAGG              CC^T[ACGT]AGG
             4181  CCTGAGG             
AseI         1166  ATTAAT               AT^TAAT
             4297  ATTAAT              
AsnI         1166  ATTAAT               AT^TAAT
             4297  ATTAAT              
Asp700I      1292  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2459  GAAGGCATTC          
BalI         3102  TGGCCA               TGG^CCA
             3853  TGGCCA              
BbsI         3911  GAAGAC               GAAGAC(2/6)
             4055  GTCTTC              
BglII         553  AGATCT               A^GATCT
             3652  AGATCT              
BpuAI        3911  GAAGAC               GAAGAC(2/6)
             4055  GTCTTC              
BsaBI        1444  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3470  GATTTGCATC          
BsaHI         728  GACGTC               G[GA]^CG[CT]C
             3459  GACGCC              
BsaWI         699  ACCGGA               [AT]^CCGG[AT]
              860  ACCGGA              
BsgI          849  GTGCAG               GTGCAG(16/14)
             2015  GTGCAG              
BsmI         2463  GCATTC               GAATGC(1/-1)
             3933  GCATTC              
BsrBI        1967  GAGCGG               CCGCTC(-3/-3)
             3092  GAGCGG              
BssSI        2357  CTCGTG               CACGAG(-5/-1)
             2496  CTCGTG              
BstBI         507  TTCGAA               TT^CGAA
             2552  TTCGAA              
Bsu36I       1393  CCTCAGG              CC^T[ACGT]AGG
             4181  CCTGAGG             
DsaI           25  CCGCGG               C^C[GA][CT]GG
             1533  CCACGG              
Eco81I       1393  CCTCAGG              CC^T[ACGT]AGG
             4181  CCTGAGG             
Hin1I         728  GACGTC               G[GA]^CG[CT]C
             3459  GACGCC              
HindIII       498  AAGCTT               A^AGCTT
             2303  AAGCTT              
MamI         1444  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             3470  GATTTGCATC          
MluNI        3102  TGGCCA               TGG^CCA
             3853  TGGCCA              
MscI         3102  TGGCCA               TGG^CCA
             3853  TGGCCA              
NspV          507  TTCGAA               TT^CGAA
             2552  TTCGAA              
PflMI         483  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1332  CCAAGGAATGG         
PshBI        1166  ATTAAT               AT^TAAT
             4297  ATTAAT              
PvuII        2969  CAGCTG               CAG^CTG
             3293  CAGCTG              
SapI         2372  GAAGAGC              GCTCTTC(1/4)
             3164  GAAGAGC             
ScaI         1115  AGTACT               AGT^ACT
             1469  AGTACT              
SexAI        2601  ACCTGGT              A^CC[AT]GGT
             3402  ACCTGGT             
SfuI          507  TTCGAA               TT^CGAA
             2552  TTCGAA              
SspI          896  AATATT               AAT^ATT
             1367  AATATT              
Van91I        483  CCACCAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1332  CCAAGGAATGG         
XmnI         1292  GAAAAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2459  GAAGGCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaII       
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
EaeI        EarI        EcoO109I    EcoRI       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII