################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BciVI BfrI BglI BlnI BsaAI BseAI BsiWI BsoBI Bsp1407I BspEI BsrGI BssHII Bst1107I BstEII BstPI BstXI BstZ17I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI HpaI KasI KpnI MfeI MluI MroI MunI NarI NcoI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacI SalI SbfI SfiI SfoI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 728 GACGTC GACGT^C Alw44I 3963 GTGCAC G^TGCAC ApaI 3096 GGGCCC GGGCC^C ApaLI 3963 GTGCAC G^TGCAC BanI 3545 GGCACC G^G[CT][GA]CC BbvCI 2125 GCTGAGG CCTCAGC(-5/-2) BcgI 324 GCACAGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 3978 TGATCA T^GATCA BlpI 3239 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3239 GCTGAGC GC^T[ACGT]AGC BsiEI 22 CGACCG CG[GA][CT]^CG BsmBI 1521 GAGACG CGTCTC(1/5) BspDI 1451 ATCGAT AT^CGAT BspHI 1065 TCATGA T^CATGA BspLU11I 4280 ACATGT A^CATGT BsrFI 17 GCCGGC [GA]^CCGG[CT] BstAPI 2347 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 3239 GCTGAGC GC^T[ACGT]AGC Cfr10I 17 GCCGGC [GA]^CCGG[CT] ClaI 1451 ATCGAT AT^CGAT DrdI 459 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3253 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 3978 TGATCA T^GATCA HaeII 3085 GGCGCT [GA]GCGC^[CT] KspI 25 CCGCGG CCGC^GG NaeI 17 GCCGGC GCC^GGC NdeI 375 CATATG CA^TATG NgoMIV 17 GCCGGC G^CCGGC NheI 3227 GCTAGC G^CTAGC NspI 4280 ACATGT [GA]CATG^[CT] RcaI 1065 TCATGA T^CATGA SacII 25 CCGCGG CCGC^GG SgrAI 16 CGCCGGCG C[GA]^CCGG[CT]G SpeI 4051 ACTAGT A^CTAGT XbaI 2690 TCTAGA T^CTAGA XcmI 2301 CCAAGCTTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AcyI 728 GACGTC G[GA]^CG[CT]C 3459 GACGCC AflIII 544 ACGTGT A^C[GA][CT]GT 4280 ACATGT AocI 1393 CCTCAGG CC^T[ACGT]AGG 4181 CCTGAGG AseI 1166 ATTAAT AT^TAAT 4297 ATTAAT AsnI 1166 ATTAAT AT^TAAT 4297 ATTAAT Asp700I 1292 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2459 GAAGGCATTC BalI 3102 TGGCCA TGG^CCA 3853 TGGCCA BbsI 3911 GAAGAC GAAGAC(2/6) 4055 GTCTTC BglII 553 AGATCT A^GATCT 3652 AGATCT BpuAI 3911 GAAGAC GAAGAC(2/6) 4055 GTCTTC BsaBI 1444 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3470 GATTTGCATC BsaHI 728 GACGTC G[GA]^CG[CT]C 3459 GACGCC BsaWI 699 ACCGGA [AT]^CCGG[AT] 860 ACCGGA BsgI 849 GTGCAG GTGCAG(16/14) 2015 GTGCAG BsmI 2463 GCATTC GAATGC(1/-1) 3933 GCATTC BsrBI 1967 GAGCGG CCGCTC(-3/-3) 3092 GAGCGG BssSI 2357 CTCGTG CACGAG(-5/-1) 2496 CTCGTG BstBI 507 TTCGAA TT^CGAA 2552 TTCGAA Bsu36I 1393 CCTCAGG CC^T[ACGT]AGG 4181 CCTGAGG DsaI 25 CCGCGG C^C[GA][CT]GG 1533 CCACGG Eco81I 1393 CCTCAGG CC^T[ACGT]AGG 4181 CCTGAGG Hin1I 728 GACGTC G[GA]^CG[CT]C 3459 GACGCC HindIII 498 AAGCTT A^AGCTT 2303 AAGCTT MamI 1444 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3470 GATTTGCATC MluNI 3102 TGGCCA TGG^CCA 3853 TGGCCA MscI 3102 TGGCCA TGG^CCA 3853 TGGCCA NspV 507 TTCGAA TT^CGAA 2552 TTCGAA PflMI 483 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1332 CCAAGGAATGG PshBI 1166 ATTAAT AT^TAAT 4297 ATTAAT PvuII 2969 CAGCTG CAG^CTG 3293 CAGCTG SapI 2372 GAAGAGC GCTCTTC(1/4) 3164 GAAGAGC ScaI 1115 AGTACT AGT^ACT 1469 AGTACT SexAI 2601 ACCTGGT A^CC[AT]GGT 3402 ACCTGGT SfuI 507 TTCGAA TT^CGAA 2552 TTCGAA SspI 896 AATATT AAT^ATT 1367 AATATT Van91I 483 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1332 CCAAGGAATGG XmnI 1292 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2459 GAAGGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII