##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccII AcyI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
AvrII BamHI BanI BbeI BbrPI
BbvCI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI FauI FseI HaeII Hin1I
HpaI KasI KpnI KspI MamI
MluI MvnI NarI NciI NcoI
NheI NlaIV NotI NruI PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PstI PvuI
RcaI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I Tth111I
Van91I XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3609 GTCTAC GT^[AC][GT]AC
AccIII 278 TCCGGA T^CCGGA
AseI 893 ATTAAT AT^TAAT
AsnI 893 ATTAAT AT^TAAT
AvaI 1701 CTCGAG C^[CT]CG[GA]G
AviII 2371 TGCGCA TGC^GCA
BanII 1815 GAGCTC G[GA]GC[CT]^C
BcgI 1703 CGAGAAAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3482 GTATCC GTATCC(6/5)
BsaI 1758 GAGACC GGTCTC(1/5)
BsaWI 278 TCCGGA [AT]^CCGG[AT]
BseAI 278 TCCGGA T^CCGGA
BsoBI 1701 CTCGAG C^[CT]CG[GA]G
Bsp1407I 453 TGTACA T^GTACA
BspEI 278 TCCGGA T^CCGGA
BsrFI 127 GCCGGC [GA]^CCGG[CT]
BsrGI 453 TGTACA T^GTACA
BstBI 266 TTCGAA TT^CGAA
BstXI 3220 CCAAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 2372 GCGC GCG^C
Cfr10I 127 GCCGGC [GA]^CCGG[CT]
EcoNI 1107 CCTAAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 67 ATGCAT ATGCA^T
FspI 2371 TGCGCA TGC^GCA
HgaI 2864 GACGC GACGC(5/10)
HhaI 2372 GCGC GCG^C
HinP1I 2372 GCGC G^CGC
MfeI 135 CAATTG C^AATTG
MroI 278 TCCGGA T^CCGGA
MunI 135 CAATTG C^AATTG
NaeI 127 GCCGGC GCC^GGC
NdeI 750 CATATG CA^TATG
NgoMIV 127 GCCGGC G^CCGGC
NsiI 67 ATGCAT ATGCA^T
NspV 266 TTCGAA TT^CGAA
PaeR7I 1701 CTCGAG C^TCGAG
PshBI 893 ATTAAT AT^TAAT
SacI 1815 GAGCTC GAGCT^C
SfuI 266 TTCGAA TT^CGAA
SspBI 453 TGTACA T^GTACA
SspI 1025 AATATT AAT^ATT
SwaI 284 ATTTAAAT ATTT^AAAT
XhoI 1701 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 304 AACGTT AA^CGTT
811 AACGTT
Alw44I 1455 GTGCAC G^TGCAC
2955 GTGCAC
ApaLI 1455 GTGCAC G^TGCAC
2955 GTGCAC
AvaII 276 GGTCC G^G[AT]CC
3460 GGACC
BlpI 1134 GCTCAGC GC^T[ACGT]AGC
2167 GCTCAGC
Bpu1102I 1134 GCTCAGC GC^T[ACGT]AGC
2167 GCTCAGC
BsmI 1437 GCATTC GAATGC(1/-1)
2374 GCATTC
BstAPI 1437 GCATTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1773 GCATCTCCTGC
BstYI 2090 GGATCT [GA]^GATC[CT]
2684 GGATCT
CelII 1134 GCTCAGC GC^T[ACGT]AGC
2167 GCTCAGC
EcoRV 766 GATATC GAT^ATC
3058 GATATC
HapII 128 CCGG C^CGG
279 CCGG
HincII 2157 GTCAAC GT[CT]^[GA]AC
2819 GTTGAC
HindII 2157 GTCAAC GT[CT]^[GA]AC
2819 GTTGAC
HindIII 1200 AAGCTT A^AGCTT
2196 AAGCTT
HpaII 128 CCGG C^CGG
279 CCGG
MflI 2090 GGATCT [GA]^GATC[CT]
2684 GGATCT
MspI 128 CCGG C^CGG
279 CCGG
NspI 69 GCATGT [GA]CATG^[CT]
1895 ACATGC
Psp1406I 304 AACGTT AA^CGTT
811 AACGTT
SapI 1845 GAAGAGC GCTCTTC(1/4)
3277 GAAGAGC
ScaI 1318 AGTACT AGT^ACT
3595 AGTACT
SfcI 2891 CTACAG C^T[GA][CT]AG
3611 CTACAG
Tsp45I 1348 GTCAC ^GT[GC]AC
3037 GTGAC
XhoII 2090 GGATCT [GA]^GATC[CT]
2684 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI BalI
BbsI BbvI BclI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XmnI