################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII Acc65I AccI AscI AseI AsnI Asp718I AspI BalI BbeI BciVI BclI BsiWI BsmI Bsp1407I BspDI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DrdI EcoRI EcoRV FbaI FseI HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PmeI PshAI PshBI PvuI RsrII SacII SalI SapI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccIII 3433 TCCGGA T^CCGGA AclI 2492 AACGTT AA^CGTT AcsI 3943 AAATTC [GA]^AATT[CT] AflII 3175 CTTAAG C^TTAAG AgeI 2086 ACCGGT A^CCGGT AhdI 354 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2994 GTGCAC G^TGCAC AocI 1496 CCTGAGG CC^T[ACGT]AGG Aor51HI 4089 AGCGCT AGC^GCT ApaLI 2994 GTGCAC G^TGCAC ApoI 3943 AAATTC [GA]^AATT[CT] Asp700I 2132 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 354 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 666 TGCGCA TGC^GCA AvrII 3386 CCTAGG C^CTAGG BamHI 823 GGATCC G^GATCC BbrPI 3710 CACGTG CAC^GTG BfrI 3175 CTTAAG C^TTAAG BglII 1681 AGATCT A^GATCT BlnI 3386 CCTAGG C^CTAGG BsaBI 1697 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3433 TCCGGA T^CCGGA BspEI 3433 TCCGGA T^CCGGA BsrFI 2086 ACCGGT [GA]^CCGG[CT] BstAPI 560 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1859 GGTGACC G^GT[ACGT]ACC BstPI 1859 GGTGACC G^GT[ACGT]ACC Bsu36I 1496 CCTGAGG CC^T[ACGT]AGG Cfr10I 2086 ACCGGT [GA]^CCGG[CT] DraI 4144 TTTAAA TTT^AAA DraIII 1244 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 2160 CGGCCG C^GGCCG Eam1105I 354 GACGAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2160 CGGCCG C^GGCCG Eco47III 4089 AGCGCT AGC^GCT Eco52I 2160 CGGCCG C^GGCCG Eco81I 1496 CCTGAGG CC^T[ACGT]AGG EcoO65I 1859 GGTGACC G^GT[ACGT]ACC EcoT22I 21 ATGCAT ATGCA^T FspI 666 TGCGCA TGC^GCA HincII 1650 GTCAAC GT[CT]^[GA]AC HindII 1650 GTCAAC GT[CT]^[GA]AC MamI 1697 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3433 TCCGGA T^CCGGA NsiI 21 ATGCAT ATGCA^T PinAI 2086 ACCGGT A^CCGGT PmaCI 3710 CACGTG CAC^GTG PmlI 3710 CACGTG CAC^GTG Psp1406I 2492 AACGTT AA^CGTT ScaI 925 AGTACT AGT^ACT XmnI 2132 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 993 GACGCC G[GA]^CG[CT]C 4027 GGCGTC BbvCI 1325 CCTCAGC CCTCAGC(-5/-2) 939 GCTGAGG BsaHI 993 GACGCC G[GA]^CG[CT]C 4027 GGCGTC BsiEI 584 CGACCG CG[GA][CT]^CG 2160 CGGCCG BsmBI 813 GAGACG CGTCTC(1/5) 1212 GAGACG BspHI 1159 TCATGA T^CATGA 3449 TCATGA BspLU11I 2068 ACATGT A^CATGT 3487 ACATGT BsrBI 3222 CCGCTC CCGCTC(-3/-3) 1749 GAGCGG EaeI 2160 CGGCCG [CT]^GGCC[GA] 2745 CGGCCA Hin1I 993 GACGCC G[GA]^CG[CT]C 4027 GGCGTC RcaI 1159 TCATGA T^CATGA 3449 TCATGA SbfI 1304 CCTGCAGG CCTGCA^GG 3183 CCTGCAGG SexAI 547 ACCTGGT A^CC[AT]GGT 3573 ACCAGGT SmaI 2178 CCCGGG CCC^GGG 3802 CCCGGG Sse8387I 1304 CCTGCAGG CCTGCA^GG 3183 CCTGCAGG XmaCI 2178 CCCGGG C^CCGGG 3802 CCCGGG XmaI 2178 CCCGGG C^CCGGG 3802 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII