##################################
# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccII AccIII AciI AflII
AgeI Alw44I AocI ApaI ApaLI
AscI Asp718I AspHI AspI AvaI
AvaII AviII AvrII BamHI BanI
BbeI BbrPI BcgI BciVI BcnI
BfrI BglI BlnI BsaAI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstPI
BstUI Bsu36I Cfr10I ClaI CpoI
DraII DraIII EagI EclXI Eco52I
Eco81I EcoO109I EcoO65I EcoRV FauI
FseI FspI HapII HgaI HpaII
KasI KpnI KspI MfeI MluI
MroI MspA1I MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NlaIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 991 GACGTC GACGT^C
AclI 4164 AACGTT AA^CGTT
AcyI 991 GACGTC G[GA]^CG[CT]C
AlwI 3636 GGATC GGATC(4/5)
AlwNI 3901 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1055 AGCGCT AGC^GCT
BalI 4730 TGGCCA TGG^CCA
BanII 3927 GAGCCC G[GA]GC[CT]^C
BbvCI 4511 GCTGAGG CCTCAGC(-5/-2)
BlpI 4467 GCTCAGC GC^T[ACGT]AGC
BmyI 3927 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 4467 GCTCAGC GC^T[ACGT]AGC
BsaHI 991 GACGTC G[GA]^CG[CT]C
BsmFI 4217 GGGAC GGGAC(10/14)
Bsp1286I 3927 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 344 TGTACA T^GTACA
BspHI 2280 TCATGA T^CATGA
BspMI 394 ACCTGC ACCTGC(4/8)
BsrGI 344 TGTACA T^GTACA
Bst1107I 562 GTATAC GTA^TAC
BstZ17I 562 GTATAC GTA^TAC
CelII 4467 GCTCAGC GC^T[ACGT]AGC
DraI 3040 TTTAAA TTT^AAA
DrdI 2562 GACATTAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1079 CCATGG C^C[GA][CT]GG
EaeI 4730 TGGCCA [CT]^GGCC[GA]
Eco47III 1055 AGCGCT AGC^GCT
EcoNI 1039 CCTTTCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 4295 ATGCAT ATGCA^T
HaeII 1055 AGCGCT [GA]GCGC^[CT]
Hin1I 991 GACGTC G[GA]^CG[CT]C
MluNI 4730 TGGCCA TGG^CCA
MscI 4730 TGGCCA TGG^CCA
NcoI 1079 CCATGG C^CATGG
NsiI 4295 ATGCAT ATGCA^T
Psp1406I 4164 AACGTT AA^CGTT
RcaI 2280 TCATGA T^CATGA
ScaI 3134 AGTACT AGT^ACT
SpeI 3714 ACTAGT A^CTAGT
SspBI 344 TGTACA T^GTACA
SspI 472 AATATT AAT^ATT
StuI 3879 AGGCCT AGG^CCT
TaqI 1900 TCGA T^CGA
TthHB8I 1900 TCGA T^CGA
XbaI 2837 TCTAGA T^CTAGA
XcmI 3132 CCAGTACTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AseI 2872 ATTAAT AT^TAAT
4345 ATTAAT
AsnI 2872 ATTAAT AT^TAAT
4345 ATTAAT
Asp700I 3328 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4704 GAAGACTTTC
BbvI 4304 GCAGC GCAGC(8/12)
4360 GCTGC
BclI 2930 TGATCA T^GATCA
4094 TGATCA
BglII 536 AGATCT A^GATCT
2183 AGATCT
BsaI 1175 GAGACC GGTCTC(1/5)
3859 GAGACC
BsgI 4105 CTGCAC GTGCAG(16/14)
4045 GTGCAG
BsmBI 2961 CGTCTC CGTCTC(1/5)
4023 CGTCTC
BspLU11I 3734 ACATGT A^CATGT
4948 ACATGT
BstXI 2689 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2887 CCATCAATATGG
CfoI 1056 GCGC GCG^C
4484 GCGC
Cfr13I 1391 GGCCC G^G[ACGT]CC
3918 GGGCC
EcoT14I 1079 CCATGG C^C[AT][AT]GG
2705 CCAAGG
FbaI 2930 TGATCA T^GATCA
4094 TGATCA
Fnu4HI 4304 GCAGC GC^[ACGT]GC
4360 GCTGC
HhaI 1056 GCGC GCG^C
4484 GCGC
HinP1I 1056 GCGC G^CGC
4484 GCGC
HpaI 972 GTTAAC GTT^AAC
4423 GTTAAC
ItaI 4304 GCAGC GC^[ACGT]GC
4360 GCTGC
NdeI 211 CATATG CA^TATG
547 CATATG
PshBI 2872 ATTAAT AT^TAAT
4345 ATTAAT
Sau96I 1391 GGCCC G^G[ACGT]CC
3918 GGGCC
SmlI 2587 CTTGAG C^T[CT][GA]AG
2722 CTTGAG
SphI 58 GCATGC GCATG^C
4480 GCATGC
StyI 1079 CCATGG C^C[AT][AT]GG
2705 CCAAGG
TseI 4304 GCAGC G^C[AT]GC
4360 GCTGC
XmnI 3328 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4704 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AcsI AfaI AflIII AhdI
AluI ApoI AspEI BbsI BfaI
BpmI BpuAI BsaBI BsaJI BsiYI
BslI BsmAI BsmI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I DdeI DpnII Eam1105I EarI
EcoRI EcoRII FokI HaeIII HincII
HindII HindIII HinfI HphI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NspI
PflMI PleI RsaI Sau3AI ScrFI
SfaNI TfiI Tru9I Tsp45I Tsp509I
TspRI Van91I XhoII