##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI Aor51HI ApaI AscI Asp718I
AvrII BalI BamHI BanI BanII
BbeI BbrPI BcgI BcnI BglI
BlnI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BsrFI BssHII BstAPI BstEII
BstPI BstUI Cfr10I ClaI CpoI
DrdI DsaI EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT22I FauI FseI HapII HgaI
Hin1I HpaII KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AclI 4607 AACGTT AA^CGTT
AflII 1745 CTTAAG C^TTAAG
AhdI 4665 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2643 GTGCAC G^TGCAC
AocI 1985 CCTGAGG CC^T[ACGT]AGG
ApaLI 2643 GTGCAC G^TGCAC
Asp700I 3304 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4665 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2643 GTGCAC G[AT]GC[AT]^C
AspI 2172 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 4641 CCCGAG C^[CT]CG[GA]G
BbvCI 3728 GCTGAGG CCTCAGC(-5/-2)
BciVI 2890 GGATAC GTATCC(6/5)
BfrI 1745 CTTAAG C^TTAAG
BglII 3735 AGATCT A^GATCT
BlpI 4094 GCTAAGC GC^T[ACGT]AGC
BmyI 2643 GTGCAC G[AGT]GC[ACT]^C
BpmI 3590 CTCCAG CTGGAG(16/14)
Bpu1102I 4094 GCTAAGC GC^T[ACGT]AGC
BsaBI 648 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 2643 GTGCAC G[AT]GC[AT]^C
BsmI 2140 GAATGC GAATGC(1/-1)
BsoBI 4641 CCCGAG C^[CT]CG[GA]G
Bsp1286I 2643 GTGCAC G[AGT]GC[ACT]^C
BspMI 3330 ACCTGC ACCTGC(4/8)
BsrDI 3882 CATTGC GCAATG(2/0)
BssSI 1281 CTCGTG CACGAG(-5/-1)
BstXI 4450 CCAATATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1985 CCTGAGG CC^T[ACGT]AGG
Cac8I 3432 GCCTGC GC[ACGT]^[ACGT]GC
CelII 4094 GCTAAGC GC^T[ACGT]AGC
DraII 4465 GGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1605 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4665 GACTGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1985 CCTGAGG CC^T[ACGT]AGG
EcoO109I 4465 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 2820 CCAAGG C^C[AT][AT]GG
HaeII 3452 GGCGCT [GA]GCGC^[CT]
HindIII 4551 AAGCTT A^AGCTT
HpaI 2741 GTTAAC GTT^AAC
MamI 648 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 795 CAGCTG C[AC]G^C[GT]G
PflFI 2172 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 4465 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 4607 AACGTT AA^CGTT
PvuII 795 CAGCTG CAG^CTG
SapI 566 GAAGAGC GCTCTTC(1/4)
ScaI 4358 AGTACT AGT^ACT
StuI 4645 AGGCCT AGG^CCT
StyI 2820 CCAAGG C^C[AT][AT]GG
SwaI 3923 ATTTAAAT ATTT^AAAT
Tth111I 2172 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3304 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 407 ATTAAT AT^TAAT
4373 ATTAAT
AsnI 407 ATTAAT AT^TAAT
4373 ATTAAT
AviII 2812 TGCGCA TGC^GCA
3106 TGCGCA
BsaI 1189 GGTCTC GGTCTC(1/5)
3270 GGTCTC
BsiYI 671 CCTCTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3218 CCTCTTAATGG
BslI 671 CCTCTCAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3218 CCTCTTAATGG
Bst1107I 3941 GTATAC GTA^TAC
4215 GTATAC
BstBI 560 TTCGAA TT^CGAA
1498 TTCGAA
BstZ17I 3941 GTATAC GTA^TAC
4215 GTATAC
FspI 2812 TGCGCA TGC^GCA
3106 TGCGCA
HaeIII 2519 GGCC GG^CC
4646 GGCC
HincII 2741 GTTAAC GT[CT]^[GA]AC
4162 GTTGAC
HindII 2741 GTTAAC GT[CT]^[GA]AC
4162 GTTGAC
NspV 560 TTCGAA TT^CGAA
1498 TTCGAA
PshBI 407 ATTAAT AT^TAAT
4373 ATTAAT
PstI 3332 CTGCAG CTGCA^G
4571 CTGCAG
SfuI 560 TTCGAA TT^CGAA
1498 TTCGAA
SpeI 473 ACTAGT A^CTAGT
1541 ACTAGT
XbaI 1438 TCTAGA T^CTAGA
1834 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AvaII
BbsI BbvI BclI BfaI BpuAI
BsaJI BseRI BsgI BsmAI BsmFI
Bsp1407I BspHI BspLU11I BsrGI BsrI
BssKI BstF5I BstNI BstYI CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRI EcoRII FbaI Fnu4HI FokI
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII